Guide for library design and bias correction for large-scale transcriptome studies using highly multiplexed RNAseq methods

BACKGROUND: Standard RNAseq methods using bulk RNA and recent single-cell RNAseq methods use DNA barcodes to identify samples and cells, and the barcoded cDNAs are pooled into a library pool before high throughput sequencing. In cases of single-cell and low-input RNAseq methods, the library is furth...

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Detalles Bibliográficos
Autores principales: Katayama, Shintaro, Skoog, Tiina, Söderhäll, Cilla, Einarsdottir, Elisabet, Krjutškov, Kaarel, Kere, Juha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693229/
https://www.ncbi.nlm.nih.gov/pubmed/31409293
http://dx.doi.org/10.1186/s12859-019-3017-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693229/
https://www.ncbi.nlm.nih.gov/pubmed/31409293
http://dx.doi.org/10.1186/s12859-019-3017-9

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