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Guide for library design and bias correction for large-scale transcriptome studies using highly multiplexed RNAseq methods
BACKGROUND: Standard RNAseq methods using bulk RNA and recent single-cell RNAseq methods use DNA barcodes to identify samples and cells, and the barcoded cDNAs are pooled into a library pool before high throughput sequencing. In cases of single-cell and low-input RNAseq methods, the library is furth...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6693229/ https://www.ncbi.nlm.nih.gov/pubmed/31409293 http://dx.doi.org/10.1186/s12859-019-3017-9 |