Cargando…

A Cell Density-Dependent Reporter in the Drosophila S2 Cells

Cell density regulates many aspects of cell properties and behaviors including metabolism, growth, cytoskeletal structure and locomotion. Importantly, the responses by cultured cells to density signals also uncover key mechanisms that govern animal development and diseases in vivo. Here we character...

Descripción completa

Detalles Bibliográficos
Autores principales: Romine, Matthew L., Li, Mo, Liu, Kevin Jiayang, Patel, Sapna K., Nelson, Julie G., Shen, Ping, Cai, Haini N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694118/
https://www.ncbi.nlm.nih.gov/pubmed/31413273
http://dx.doi.org/10.1038/s41598-019-47652-0
_version_ 1783443780230184960
author Romine, Matthew L.
Li, Mo
Liu, Kevin Jiayang
Patel, Sapna K.
Nelson, Julie G.
Shen, Ping
Cai, Haini N.
author_facet Romine, Matthew L.
Li, Mo
Liu, Kevin Jiayang
Patel, Sapna K.
Nelson, Julie G.
Shen, Ping
Cai, Haini N.
author_sort Romine, Matthew L.
collection PubMed
description Cell density regulates many aspects of cell properties and behaviors including metabolism, growth, cytoskeletal structure and locomotion. Importantly, the responses by cultured cells to density signals also uncover key mechanisms that govern animal development and diseases in vivo. Here we characterized a density-responsive reporter system in transgenic Drosophila S2 cells. We show that the reporter genes are strongly induced in a cell density-dependent and reporter-independent fashion. The rapid and reversible induction occurs at the level of mRNA accumulation. We show that multiple DNA elements within the transgene sequences, including a metal response element from the metallothionein gene, contribute to the reporter induction. The reporter induction correlates with changes in multiple cell density and growth regulatory pathways including hypoxia, apoptosis, cell cycle and cytoskeletal organization. Potential applications of such a density-responsive reporter will be discussed.
format Online
Article
Text
id pubmed-6694118
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-66941182019-08-19 A Cell Density-Dependent Reporter in the Drosophila S2 Cells Romine, Matthew L. Li, Mo Liu, Kevin Jiayang Patel, Sapna K. Nelson, Julie G. Shen, Ping Cai, Haini N. Sci Rep Article Cell density regulates many aspects of cell properties and behaviors including metabolism, growth, cytoskeletal structure and locomotion. Importantly, the responses by cultured cells to density signals also uncover key mechanisms that govern animal development and diseases in vivo. Here we characterized a density-responsive reporter system in transgenic Drosophila S2 cells. We show that the reporter genes are strongly induced in a cell density-dependent and reporter-independent fashion. The rapid and reversible induction occurs at the level of mRNA accumulation. We show that multiple DNA elements within the transgene sequences, including a metal response element from the metallothionein gene, contribute to the reporter induction. The reporter induction correlates with changes in multiple cell density and growth regulatory pathways including hypoxia, apoptosis, cell cycle and cytoskeletal organization. Potential applications of such a density-responsive reporter will be discussed. Nature Publishing Group UK 2019-08-14 /pmc/articles/PMC6694118/ /pubmed/31413273 http://dx.doi.org/10.1038/s41598-019-47652-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Romine, Matthew L.
Li, Mo
Liu, Kevin Jiayang
Patel, Sapna K.
Nelson, Julie G.
Shen, Ping
Cai, Haini N.
A Cell Density-Dependent Reporter in the Drosophila S2 Cells
title A Cell Density-Dependent Reporter in the Drosophila S2 Cells
title_full A Cell Density-Dependent Reporter in the Drosophila S2 Cells
title_fullStr A Cell Density-Dependent Reporter in the Drosophila S2 Cells
title_full_unstemmed A Cell Density-Dependent Reporter in the Drosophila S2 Cells
title_short A Cell Density-Dependent Reporter in the Drosophila S2 Cells
title_sort cell density-dependent reporter in the drosophila s2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694118/
https://www.ncbi.nlm.nih.gov/pubmed/31413273
http://dx.doi.org/10.1038/s41598-019-47652-0
work_keys_str_mv AT rominematthewl acelldensitydependentreporterinthedrosophilas2cells
AT limo acelldensitydependentreporterinthedrosophilas2cells
AT liukevinjiayang acelldensitydependentreporterinthedrosophilas2cells
AT patelsapnak acelldensitydependentreporterinthedrosophilas2cells
AT nelsonjulieg acelldensitydependentreporterinthedrosophilas2cells
AT shenping acelldensitydependentreporterinthedrosophilas2cells
AT caihainin acelldensitydependentreporterinthedrosophilas2cells
AT rominematthewl celldensitydependentreporterinthedrosophilas2cells
AT limo celldensitydependentreporterinthedrosophilas2cells
AT liukevinjiayang celldensitydependentreporterinthedrosophilas2cells
AT patelsapnak celldensitydependentreporterinthedrosophilas2cells
AT nelsonjulieg celldensitydependentreporterinthedrosophilas2cells
AT shenping celldensitydependentreporterinthedrosophilas2cells
AT caihainin celldensitydependentreporterinthedrosophilas2cells