Cargando…

Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip

BACKGROUND: Glaesserella parasuis (G. parasuis) is an influential pathogen of the pig, which induces high morbidity and mortality in naive pig populations in the pig industry. Accurate and rapid detection of the agent is important for disease control. In this study, a simple recombinase polymerase a...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Ting-ting, Liu, Meng-zhi, Yin, Rong-huan, Yao, Long-quan, Liu, Bao-shan, Chen, Ze-liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694577/
https://www.ncbi.nlm.nih.gov/pubmed/31412870
http://dx.doi.org/10.1186/s12917-019-2039-x
_version_ 1783443853673496576
author Zhang, Ting-ting
Liu, Meng-zhi
Yin, Rong-huan
Yao, Long-quan
Liu, Bao-shan
Chen, Ze-liang
author_facet Zhang, Ting-ting
Liu, Meng-zhi
Yin, Rong-huan
Yao, Long-quan
Liu, Bao-shan
Chen, Ze-liang
author_sort Zhang, Ting-ting
collection PubMed
description BACKGROUND: Glaesserella parasuis (G. parasuis) is an influential pathogen of the pig, which induces high morbidity and mortality in naive pig populations in the pig industry. Accurate and rapid detection of the agent is important for disease control. In this study, a simple recombinase polymerase amplification (RPA) with a Lateral flow (LF) strip (RPA-LF-GPS) was developed to detect G. parasuis. RESULTS: The RPA-LF-GPS can specifically detect G. parasuis a limit of 100 CFU from other common related pathogens causing arthritis in the pig. The RPA-LF-GPS assay can use boiled synovial fluid samples as a template with the same sensitivity as other DNA extraction methods. In the detection of clinic positive synovial fluid sample, RPA-LF-GPS is equally sensitive (98.1%) compared with that of PCR (90.4%) (P > 0.05). The whole procedure of the RPA-LF-GPS assay could be finished in 1 hour without professional equipment. CONCLUSIONS: RPA-LF-GPS assay is a rapid and simple method for point-of-care diagnostic testing for G. parasuis infection.
format Online
Article
Text
id pubmed-6694577
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-66945772019-08-19 Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip Zhang, Ting-ting Liu, Meng-zhi Yin, Rong-huan Yao, Long-quan Liu, Bao-shan Chen, Ze-liang BMC Vet Res Research Article BACKGROUND: Glaesserella parasuis (G. parasuis) is an influential pathogen of the pig, which induces high morbidity and mortality in naive pig populations in the pig industry. Accurate and rapid detection of the agent is important for disease control. In this study, a simple recombinase polymerase amplification (RPA) with a Lateral flow (LF) strip (RPA-LF-GPS) was developed to detect G. parasuis. RESULTS: The RPA-LF-GPS can specifically detect G. parasuis a limit of 100 CFU from other common related pathogens causing arthritis in the pig. The RPA-LF-GPS assay can use boiled synovial fluid samples as a template with the same sensitivity as other DNA extraction methods. In the detection of clinic positive synovial fluid sample, RPA-LF-GPS is equally sensitive (98.1%) compared with that of PCR (90.4%) (P > 0.05). The whole procedure of the RPA-LF-GPS assay could be finished in 1 hour without professional equipment. CONCLUSIONS: RPA-LF-GPS assay is a rapid and simple method for point-of-care diagnostic testing for G. parasuis infection. BioMed Central 2019-08-14 /pmc/articles/PMC6694577/ /pubmed/31412870 http://dx.doi.org/10.1186/s12917-019-2039-x Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Ting-ting
Liu, Meng-zhi
Yin, Rong-huan
Yao, Long-quan
Liu, Bao-shan
Chen, Ze-liang
Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip
title Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip
title_full Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip
title_fullStr Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip
title_full_unstemmed Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip
title_short Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip
title_sort rapid and simple detection of glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694577/
https://www.ncbi.nlm.nih.gov/pubmed/31412870
http://dx.doi.org/10.1186/s12917-019-2039-x
work_keys_str_mv AT zhangtingting rapidandsimpledetectionofglaesserellaparasuisinsynovialfluidbyrecombinasepolymeraseamplificationandlateralflowstrip
AT liumengzhi rapidandsimpledetectionofglaesserellaparasuisinsynovialfluidbyrecombinasepolymeraseamplificationandlateralflowstrip
AT yinronghuan rapidandsimpledetectionofglaesserellaparasuisinsynovialfluidbyrecombinasepolymeraseamplificationandlateralflowstrip
AT yaolongquan rapidandsimpledetectionofglaesserellaparasuisinsynovialfluidbyrecombinasepolymeraseamplificationandlateralflowstrip
AT liubaoshan rapidandsimpledetectionofglaesserellaparasuisinsynovialfluidbyrecombinasepolymeraseamplificationandlateralflowstrip
AT chenzeliang rapidandsimpledetectionofglaesserellaparasuisinsynovialfluidbyrecombinasepolymeraseamplificationandlateralflowstrip