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Kinetic assay of starvation sensitivity in yeast autophagy mutants allows for the identification of intermediary phenotypes
OBJECTIVE: A classical method to quantitatively determine the starvation sensitivity phenotype of autophagy mutant budding yeast strains is to starve them for a period of time and then to assess the proportion of cells that retain the ability to form colonies when the availability of nutrients is re...
Autores principales: | Sturgeon, Candyce M., Robinson, Meaghan R., Penton, Molly C., Clemmer, Deanna C., Trujillo, Maria A., Khawaja, Ambar U., Segarra, Verónica A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694668/ https://www.ncbi.nlm.nih.gov/pubmed/31412956 http://dx.doi.org/10.1186/s13104-019-4545-0 |
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