Cargando…

EditR: A Method to Quantify Base Editing from Sanger Sequencing

CRISPR-Cas9-Cytidine deaminase fusion enzymes—termed “base editors”—allow targeted editing of genomic deoxycytidine to deoxythymidine (C:G→T:A) without the need for double-stranded break induction. Base editors represent a paradigm shift in gene editing technology due to their unprecedented efficien...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kluesner, Mitchell G., Nedveck, Derek A., Lahr, Walker S., Garbe, John R., Abrahante, Juan E., Webber, Beau R., Moriarity, Branden S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Mary Ann Liebert, Inc. 2018
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694769/ https://www.ncbi.nlm.nih.gov/pubmed/31021262 http://dx.doi.org/10.1089/crispr.2018.0014

Ejemplares similares

MultiEditR: The first tool for the detection and quantification of RNA editing from Sanger sequencing demonstrates comparable fidelity to RNA-seq
por: Kluesner, Mitchell G., et al.
Publicado: (2021)

CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
por: Kluesner, Mitchell G., et al.
Publicado: (2021)

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays
por: Kurata, Morito, et al.
Publicado: (2018)

Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease
por: Johnson, Matthew J., et al.
Publicado: (2018)

SangeR: the high-throughput Sanger sequencing analysis pipeline
por: Schmid, Kai, et al.
Publicado: (2022)

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
por: Webber, Beau R., et al.
Publicado: (2019)

Author Correction: Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
por: Webber, Beau R., et al.
Publicado: (2019)

Deconvolution of Complex DNA Repair (DECODR): Establishing a Novel Deconvolution Algorithm for Comprehensive Analysis of CRISPR-Edited Sanger Sequencing Data
por: Bloh, Kevin, et al.
Publicado: (2021)

Myosin Heavy Chain Converter Domain Mutations Drive Early-Stage Changes in Extracellular Matrix Dynamics in Hypertrophic Cardiomyopathy
por: Hsieh, Jeanne, et al.
Publicado: (2022)

Correction of Fanconi Anemia Mutations Using Digital Genome Engineering
por: Sipe, Christopher J., et al.
Publicado: (2022)

qSanger: Quantification of Genetic Variants in Bacterial Cultures by Sanger Sequencing
por: Prakash, Satya, et al.
Publicado: (2023)

A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers
por: Wong, Derek P., et al.
Publicado: (2022)

Quantifying RNA Editing in Deep Transcriptome Datasets
por: Lo Giudice, Claudio, et al.
Publicado: (2020)

sangeranalyseR: Simple and Interactive Processing of Sanger Sequencing Data in R
por: Chao, Kuan-Hao, et al.
Publicado: (2021)

Germline T cell receptor exchange results in physiological T cell development and function
por: Rollins, Meagan R., et al.
Publicado: (2023)

A Pan-RNase Inhibitor Enabling CRISPR-mRNA Platforms for Engineering of Primary Human Monocytes
por: Laoharawee, Kanut, et al.
Publicado: (2022)

254 A systems genomics approach to identify novel drug targets of Ewing sarcoma through ancestry-informed human iPSC modeling
por: Moss, Rachel M, et al.
Publicado: (2023)

A Sequel to Sanger: amplicon sequencing that scales
por: Hebert, Paul D. N., et al.
Publicado: (2018)

Fluid mechanics for civil engineers: SI edition
por: Webber, N B
Publicado: (1990)

A Python script to merge Sanger sequences
por: Chen, Cen, et al.
Publicado: (2021)

High-throughput sequencing of partially edited trypanosome mRNAs reveals barriers to editing progression and evidence for alternative editing
por: Simpson, Rachel M., et al.
Publicado: (2016)

The origin of unwanted editing byproducts in gene editing: Origin of unwanted editing byproducts in gene editing
por: Yin, Jianhang, et al.
Publicado: (2022)

Rapid Sanger Sequencing of the 16S rRNA Gene for Identification of Some Common Pathogens
por: Chen, Linxiang, et al.
Publicado: (2014)

The Molecular Imaging of Natural Killer Cells
por: Shapovalova, Mariya, et al.
Publicado: (2018)

Engineering T cells to enhance 3D migration through structurally and mechanically complex tumor microenvironments
por: Tabdanov, Erdem D., et al.
Publicado: (2021)

Estimating Copy-Number Proportions: The Comeback of Sanger Sequencing
por: Seroussi, Eyal
Publicado: (2021)

Robert M. Sanger
Publicado: (1911)

Professor Max Sänger
Publicado: (1903)

Sänger’s Cæsarean Operation
Publicado: (1886)

Dissecting and tuning primer editing by proofreading polymerases
por: Gohl, Daryl M, et al.
Publicado: (2021)

Ebola Virus RNA Editing Depends on the Primary Editing Site Sequence and an Upstream Secondary Structure
por: Mehedi, Masfique, et al.
Publicado: (2013)

Base editing and prime editing in laboratory animals
por: Caso, Federico, et al.
Publicado: (2021)

Prime Editing: An All-Rounder for Genome Editing
por: Lu, Chenyu, et al.
Publicado: (2022)

Whole genome sequencing of CCR5 CRISPR-Cas9-edited Mauritian cynomolgus macaque blastomeres reveals large-scale deletions and off-target edits
por: Schmidt, Jenna Kropp, et al.
Publicado: (2023)

AlleleProfileR: A versatile tool to identify and profile sequence variants in edited genomes
por: Bruyneel, Arne A. N., et al.
Publicado: (2019)

Sequence analysis and editing for bisulphite genomic sequencing projects
por: Carr, Ian M., et al.
Publicado: (2007)

Altered Regulation of adipomiR Editing with Aging
por: Meadows, Sabel, et al.
Publicado: (2020)

AutoSeqMan: batch assembly of contigs for Sanger sequences
por: Jin, Jie-Qiong, et al.
Publicado: (2018)

CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing
por: Dehairs, Jonas, et al.
Publicado: (2016)

PHFinder: assisted detection of point heteroplasmy in Sanger sequencing chromatograms
por: Suárez Menéndez, Marcos, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_2e4n5ujrv57vegkjsgoudakqo6