Chromatographic and computational studies of ligands associated with bilharziasis

OBJECTIVE: Rapid diagnostic techniques that do not depend on microscopic analysis are urgently needed for rapid diagnosis and management of bilharziasis. Specific ligands that are excreted through urine in bilharziasis may serve as rapid diagnostic biomarkers to replace microscopy, which is cumbersome and time-consuming. The aim of this study was to identify ligands associated with bilharziasis. METHODS: Microscopy was employed to detect ova of Schistosoma haematobium in urine specimens obtained from 1032 subjects. Pooled positive urine samples and pooled normal urine samples were separately prepared in triplicates and analyzed by gas chromatography-mass spectrophotometry (GC-MS). Ligands identified in each pool were noted. Computational analysis was performed between the schistosome receptors and ligands. RESULTS: GC-MS revealed that the level of indole in bilharziasis sample was higher than that in normal urine. Indole was the ligand with the highest (28.63%) concentration in the pooled positive urine sample, while ethyl phenazone level was the highest (69.64%) in the pooled normal sample. Computational analysis depicted perfect docking with indole and all other ligands identified in positive urine samples. CONCLUSION: This study identified some ligands associated with bilharziasis some unique to normal (negative) urine samples.