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Short-term influences of radiation on musculofascial healing in a laparotomy rat model
Preoperative radiation is associated with an increased risk of wound complications. However, the influences of radiation on musculofascial wound healing remains unclear. The purpose of the study was to investigate the short-term effects of preoperative local radiation on the musculofascial healing o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695398/ https://www.ncbi.nlm.nih.gov/pubmed/31417127 http://dx.doi.org/10.1038/s41598-019-48201-5 |
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author | Chen, Youbai Zhang, Qixu Wu, Yewen Branch-Brooks, Cynthia D. Butler, Charles E. |
author_facet | Chen, Youbai Zhang, Qixu Wu, Yewen Branch-Brooks, Cynthia D. Butler, Charles E. |
author_sort | Chen, Youbai |
collection | PubMed |
description | Preoperative radiation is associated with an increased risk of wound complications. However, the influences of radiation on musculofascial wound healing remains unclear. The purpose of the study was to investigate the short-term effects of preoperative local radiation on the musculofascial healing of laparotomy incisions in a rat model. Eighteen Fischer 344 rats received radiation doses of 0, 10, or 20 Gy to the abdominal wall and underwent laparotomy 4 weeks later. Two weeks after laparotomy, samples of irradiated muscle were harvested for mechanical tests, histological (Hematoxylin & Eosin, and Masson’s Trichrome) and immunohistochemical analyses using KI67, CD31, TGF-β, and MYOD1 antibodies. The elastic modulus (EM), maximum strain (MS), and ultimate tensile strength (UTS) in the 20-Gy group were significantly weaker than those in the 0-Gy group. The EM and UTS in the 20-Gy group were significantly lower than those in the 10-Gy group. The UTS and MS in the 10-Gy group were significantly lower than those in the 0-Gy group. The mean number of inflammatory cells per mm(2) in the 20-Gy group was significantly larger than those in the 10- and 0-Gy groups. The mean numbers of CD31-, KI67-, and MYOD1-positive cells, the optical density of TGF-β, and the microvessel density in the 20-Gy group were significantly smaller than those in the 10- and 0-Gy groups. These results indicated that radiation delays musculofascial healing and decreases mechanical strength of the laparotomy incision by creating a chronic inflammatory environment, inhibiting cell proliferation, angiogenesis, granulation maturation, collagen deposition, and muscular regeneration in a dose-dependent manner. The impaired biomechanical, histological and molecular properties may be associated with the higher risk of wound complications in patients who undergo radiotherapy prior to laparotomy. |
format | Online Article Text |
id | pubmed-6695398 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66953982019-08-19 Short-term influences of radiation on musculofascial healing in a laparotomy rat model Chen, Youbai Zhang, Qixu Wu, Yewen Branch-Brooks, Cynthia D. Butler, Charles E. Sci Rep Article Preoperative radiation is associated with an increased risk of wound complications. However, the influences of radiation on musculofascial wound healing remains unclear. The purpose of the study was to investigate the short-term effects of preoperative local radiation on the musculofascial healing of laparotomy incisions in a rat model. Eighteen Fischer 344 rats received radiation doses of 0, 10, or 20 Gy to the abdominal wall and underwent laparotomy 4 weeks later. Two weeks after laparotomy, samples of irradiated muscle were harvested for mechanical tests, histological (Hematoxylin & Eosin, and Masson’s Trichrome) and immunohistochemical analyses using KI67, CD31, TGF-β, and MYOD1 antibodies. The elastic modulus (EM), maximum strain (MS), and ultimate tensile strength (UTS) in the 20-Gy group were significantly weaker than those in the 0-Gy group. The EM and UTS in the 20-Gy group were significantly lower than those in the 10-Gy group. The UTS and MS in the 10-Gy group were significantly lower than those in the 0-Gy group. The mean number of inflammatory cells per mm(2) in the 20-Gy group was significantly larger than those in the 10- and 0-Gy groups. The mean numbers of CD31-, KI67-, and MYOD1-positive cells, the optical density of TGF-β, and the microvessel density in the 20-Gy group were significantly smaller than those in the 10- and 0-Gy groups. These results indicated that radiation delays musculofascial healing and decreases mechanical strength of the laparotomy incision by creating a chronic inflammatory environment, inhibiting cell proliferation, angiogenesis, granulation maturation, collagen deposition, and muscular regeneration in a dose-dependent manner. The impaired biomechanical, histological and molecular properties may be associated with the higher risk of wound complications in patients who undergo radiotherapy prior to laparotomy. Nature Publishing Group UK 2019-08-15 /pmc/articles/PMC6695398/ /pubmed/31417127 http://dx.doi.org/10.1038/s41598-019-48201-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chen, Youbai Zhang, Qixu Wu, Yewen Branch-Brooks, Cynthia D. Butler, Charles E. Short-term influences of radiation on musculofascial healing in a laparotomy rat model |
title | Short-term influences of radiation on musculofascial healing in a laparotomy rat model |
title_full | Short-term influences of radiation on musculofascial healing in a laparotomy rat model |
title_fullStr | Short-term influences of radiation on musculofascial healing in a laparotomy rat model |
title_full_unstemmed | Short-term influences of radiation on musculofascial healing in a laparotomy rat model |
title_short | Short-term influences of radiation on musculofascial healing in a laparotomy rat model |
title_sort | short-term influences of radiation on musculofascial healing in a laparotomy rat model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695398/ https://www.ncbi.nlm.nih.gov/pubmed/31417127 http://dx.doi.org/10.1038/s41598-019-48201-5 |
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