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Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi

Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phe...

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Autores principales: Ghodke, Amol Bharat, Good, Robert Trygve, Golz, John F., Russell, Derek A., Edwards, Owain, Robin, Charles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695413/
https://www.ncbi.nlm.nih.gov/pubmed/31417162
http://dx.doi.org/10.1038/s41598-019-47357-4
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author Ghodke, Amol Bharat
Good, Robert Trygve
Golz, John F.
Russell, Derek A.
Edwards, Owain
Robin, Charles
author_facet Ghodke, Amol Bharat
Good, Robert Trygve
Golz, John F.
Russell, Derek A.
Edwards, Owain
Robin, Charles
author_sort Ghodke, Amol Bharat
collection PubMed
description Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phenotypic effects. While these studies suggest that RNA interference (RNAi) might be used to suppress aphid populations, none have shown effects sufficient for field control. The current study analyses the efficacy of dsRNA directed against previously reported gene-targets on Green peach aphid (Myzus persicae) strains. No silencing effect was observed when dsRNA was delivered in artificial diet with or without transfection reagents. dsRNA produced in planta also failed to induce significant RNAi in M. persicae. Transcriptome analyses of the midgut suggested other potential targets including the Ferritin heavy chain transcripts, but they also could not be knocked down with dsRNA. Here we show that dsRNA is rapidly degraded by midgut secretions of Myzus persicae. Analysis of the transcriptome of the M. persicae midgut revealed that an ortholog of RNases from other insects was abundant.
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spelling pubmed-66954132019-08-19 Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi Ghodke, Amol Bharat Good, Robert Trygve Golz, John F. Russell, Derek A. Edwards, Owain Robin, Charles Sci Rep Article Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phenotypic effects. While these studies suggest that RNA interference (RNAi) might be used to suppress aphid populations, none have shown effects sufficient for field control. The current study analyses the efficacy of dsRNA directed against previously reported gene-targets on Green peach aphid (Myzus persicae) strains. No silencing effect was observed when dsRNA was delivered in artificial diet with or without transfection reagents. dsRNA produced in planta also failed to induce significant RNAi in M. persicae. Transcriptome analyses of the midgut suggested other potential targets including the Ferritin heavy chain transcripts, but they also could not be knocked down with dsRNA. Here we show that dsRNA is rapidly degraded by midgut secretions of Myzus persicae. Analysis of the transcriptome of the M. persicae midgut revealed that an ortholog of RNases from other insects was abundant. Nature Publishing Group UK 2019-08-15 /pmc/articles/PMC6695413/ /pubmed/31417162 http://dx.doi.org/10.1038/s41598-019-47357-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ghodke, Amol Bharat
Good, Robert Trygve
Golz, John F.
Russell, Derek A.
Edwards, Owain
Robin, Charles
Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_full Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_fullStr Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_full_unstemmed Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_short Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
title_sort extracellular endonucleases in the midgut of myzus persicae may limit the efficacy of orally delivered rnai
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695413/
https://www.ncbi.nlm.nih.gov/pubmed/31417162
http://dx.doi.org/10.1038/s41598-019-47357-4
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