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Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695413/ https://www.ncbi.nlm.nih.gov/pubmed/31417162 http://dx.doi.org/10.1038/s41598-019-47357-4 |
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author | Ghodke, Amol Bharat Good, Robert Trygve Golz, John F. Russell, Derek A. Edwards, Owain Robin, Charles |
author_facet | Ghodke, Amol Bharat Good, Robert Trygve Golz, John F. Russell, Derek A. Edwards, Owain Robin, Charles |
author_sort | Ghodke, Amol Bharat |
collection | PubMed |
description | Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phenotypic effects. While these studies suggest that RNA interference (RNAi) might be used to suppress aphid populations, none have shown effects sufficient for field control. The current study analyses the efficacy of dsRNA directed against previously reported gene-targets on Green peach aphid (Myzus persicae) strains. No silencing effect was observed when dsRNA was delivered in artificial diet with or without transfection reagents. dsRNA produced in planta also failed to induce significant RNAi in M. persicae. Transcriptome analyses of the midgut suggested other potential targets including the Ferritin heavy chain transcripts, but they also could not be knocked down with dsRNA. Here we show that dsRNA is rapidly degraded by midgut secretions of Myzus persicae. Analysis of the transcriptome of the M. persicae midgut revealed that an ortholog of RNases from other insects was abundant. |
format | Online Article Text |
id | pubmed-6695413 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66954132019-08-19 Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi Ghodke, Amol Bharat Good, Robert Trygve Golz, John F. Russell, Derek A. Edwards, Owain Robin, Charles Sci Rep Article Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phenotypic effects. While these studies suggest that RNA interference (RNAi) might be used to suppress aphid populations, none have shown effects sufficient for field control. The current study analyses the efficacy of dsRNA directed against previously reported gene-targets on Green peach aphid (Myzus persicae) strains. No silencing effect was observed when dsRNA was delivered in artificial diet with or without transfection reagents. dsRNA produced in planta also failed to induce significant RNAi in M. persicae. Transcriptome analyses of the midgut suggested other potential targets including the Ferritin heavy chain transcripts, but they also could not be knocked down with dsRNA. Here we show that dsRNA is rapidly degraded by midgut secretions of Myzus persicae. Analysis of the transcriptome of the M. persicae midgut revealed that an ortholog of RNases from other insects was abundant. Nature Publishing Group UK 2019-08-15 /pmc/articles/PMC6695413/ /pubmed/31417162 http://dx.doi.org/10.1038/s41598-019-47357-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ghodke, Amol Bharat Good, Robert Trygve Golz, John F. Russell, Derek A. Edwards, Owain Robin, Charles Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi |
title | Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi |
title_full | Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi |
title_fullStr | Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi |
title_full_unstemmed | Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi |
title_short | Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi |
title_sort | extracellular endonucleases in the midgut of myzus persicae may limit the efficacy of orally delivered rnai |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695413/ https://www.ncbi.nlm.nih.gov/pubmed/31417162 http://dx.doi.org/10.1038/s41598-019-47357-4 |
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