Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells

Fluorescent probes such as thiol-reactive and Ni(2+)-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imag...

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Detalles Bibliográficos
Autores principales: Jiang, Nan, Li, Hongyan, Sun, Hongzhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695521/
https://www.ncbi.nlm.nih.gov/pubmed/31448265
http://dx.doi.org/10.3389/fchem.2019.00560
Descripción
Sumario:Fluorescent probes such as thiol-reactive and Ni(2+)-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imaging and recognition. We then summarize a family of metal-NTA based fluorescence probes in the visualization of His(6)-tagged protein and identification of metalloproteins at proteome-wide scale. The pros and cons of the probes, as well as ways to optimize them, are discussed.

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