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Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells
Fluorescent probes such as thiol-reactive and Ni(2+)-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imag...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695521/ https://www.ncbi.nlm.nih.gov/pubmed/31448265 http://dx.doi.org/10.3389/fchem.2019.00560 |
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author | Jiang, Nan Li, Hongyan Sun, Hongzhe |
author_facet | Jiang, Nan Li, Hongyan Sun, Hongzhe |
author_sort | Jiang, Nan |
collection | PubMed |
description | Fluorescent probes such as thiol-reactive and Ni(2+)-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imaging and recognition. We then summarize a family of metal-NTA based fluorescence probes in the visualization of His(6)-tagged protein and identification of metalloproteins at proteome-wide scale. The pros and cons of the probes, as well as ways to optimize them, are discussed. |
format | Online Article Text |
id | pubmed-6695521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66955212019-08-23 Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells Jiang, Nan Li, Hongyan Sun, Hongzhe Front Chem Chemistry Fluorescent probes such as thiol-reactive and Ni(2+)-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imaging and recognition. We then summarize a family of metal-NTA based fluorescence probes in the visualization of His(6)-tagged protein and identification of metalloproteins at proteome-wide scale. The pros and cons of the probes, as well as ways to optimize them, are discussed. Frontiers Media S.A. 2019-08-09 /pmc/articles/PMC6695521/ /pubmed/31448265 http://dx.doi.org/10.3389/fchem.2019.00560 Text en Copyright © 2019 Jiang, Li and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Jiang, Nan Li, Hongyan Sun, Hongzhe Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells |
title | Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells |
title_full | Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells |
title_fullStr | Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells |
title_full_unstemmed | Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells |
title_short | Recognition of Proteins by Metal Chelation-Based Fluorescent Probes in Cells |
title_sort | recognition of proteins by metal chelation-based fluorescent probes in cells |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695521/ https://www.ncbi.nlm.nih.gov/pubmed/31448265 http://dx.doi.org/10.3389/fchem.2019.00560 |
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