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Highly Selective, Aptamer-Based, Ultrasensitive Nanogold Colorimetric Smartphone Readout for Detection of Cd(II)

A highly selective and sensitive method for Cd(II) detection was developed based on aptamer and gold nanoparticles (AuNPs) combined with a colorimetric smartphone readout. The experimental conditions such as reaction time of polydiene dimethyl ammonium chloride (PDDA) and AuNPs, PDDA dose, time of a...

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Autores principales: Xu, Lu, Liang, Jun, Wang, Yonghui, Ren, Shuyue, Wu, Jin, Zhou, Huanying, Gao, Zhixian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695641/
https://www.ncbi.nlm.nih.gov/pubmed/31362377
http://dx.doi.org/10.3390/molecules24152745
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author Xu, Lu
Liang, Jun
Wang, Yonghui
Ren, Shuyue
Wu, Jin
Zhou, Huanying
Gao, Zhixian
author_facet Xu, Lu
Liang, Jun
Wang, Yonghui
Ren, Shuyue
Wu, Jin
Zhou, Huanying
Gao, Zhixian
author_sort Xu, Lu
collection PubMed
description A highly selective and sensitive method for Cd(II) detection was developed based on aptamer and gold nanoparticles (AuNPs) combined with a colorimetric smartphone readout. The experimental conditions such as reaction time of polydiene dimethyl ammonium chloride (PDDA) and AuNPs, PDDA dose, time of aptamer and PDDA incubation, and aptamer concentration were optimized. Under the optimized conditions, the color and red(R) value of the solution was concentration-dependent on Cd(II). The proposed method exhibited a linear range of 1–400 ng/mL (r(2) = 0.9794) with a limit of detection (LOD) of 1 ng/mL. This method had been successfully applied to test and quantify Cd(II) in water and rice samples, and the results were in full agreement with those from the atomic absorption spectrometer. Therefore, low-cost colorimetry demonstrated its potential for practical application in visual or quantitative detection with a smartphone. This approach can be readily applied to other analytes.
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spelling pubmed-66956412019-09-05 Highly Selective, Aptamer-Based, Ultrasensitive Nanogold Colorimetric Smartphone Readout for Detection of Cd(II) Xu, Lu Liang, Jun Wang, Yonghui Ren, Shuyue Wu, Jin Zhou, Huanying Gao, Zhixian Molecules Article A highly selective and sensitive method for Cd(II) detection was developed based on aptamer and gold nanoparticles (AuNPs) combined with a colorimetric smartphone readout. The experimental conditions such as reaction time of polydiene dimethyl ammonium chloride (PDDA) and AuNPs, PDDA dose, time of aptamer and PDDA incubation, and aptamer concentration were optimized. Under the optimized conditions, the color and red(R) value of the solution was concentration-dependent on Cd(II). The proposed method exhibited a linear range of 1–400 ng/mL (r(2) = 0.9794) with a limit of detection (LOD) of 1 ng/mL. This method had been successfully applied to test and quantify Cd(II) in water and rice samples, and the results were in full agreement with those from the atomic absorption spectrometer. Therefore, low-cost colorimetry demonstrated its potential for practical application in visual or quantitative detection with a smartphone. This approach can be readily applied to other analytes. MDPI 2019-07-29 /pmc/articles/PMC6695641/ /pubmed/31362377 http://dx.doi.org/10.3390/molecules24152745 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Lu
Liang, Jun
Wang, Yonghui
Ren, Shuyue
Wu, Jin
Zhou, Huanying
Gao, Zhixian
Highly Selective, Aptamer-Based, Ultrasensitive Nanogold Colorimetric Smartphone Readout for Detection of Cd(II)
title Highly Selective, Aptamer-Based, Ultrasensitive Nanogold Colorimetric Smartphone Readout for Detection of Cd(II)
title_full Highly Selective, Aptamer-Based, Ultrasensitive Nanogold Colorimetric Smartphone Readout for Detection of Cd(II)
title_fullStr Highly Selective, Aptamer-Based, Ultrasensitive Nanogold Colorimetric Smartphone Readout for Detection of Cd(II)
title_full_unstemmed Highly Selective, Aptamer-Based, Ultrasensitive Nanogold Colorimetric Smartphone Readout for Detection of Cd(II)
title_short Highly Selective, Aptamer-Based, Ultrasensitive Nanogold Colorimetric Smartphone Readout for Detection of Cd(II)
title_sort highly selective, aptamer-based, ultrasensitive nanogold colorimetric smartphone readout for detection of cd(ii)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695641/
https://www.ncbi.nlm.nih.gov/pubmed/31362377
http://dx.doi.org/10.3390/molecules24152745
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