Cargando…

Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in Myzus persicae

Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insec...

Descripción completa

Detalles Bibliográficos
Autores principales: Pan, Yiou, Xu, Pengjun, Zeng, Xiaochun, Liu, Xuemei, Shang, Qingli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695686/
https://www.ncbi.nlm.nih.gov/pubmed/31349586
http://dx.doi.org/10.3390/ijms20153637
_version_ 1783444093512187904
author Pan, Yiou
Xu, Pengjun
Zeng, Xiaochun
Liu, Xuemei
Shang, Qingli
author_facet Pan, Yiou
Xu, Pengjun
Zeng, Xiaochun
Liu, Xuemei
Shang, Qingli
author_sort Pan, Yiou
collection PubMed
description Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of UGTs and other detoxification enzymes. However, UGT functions in green peach aphid (Myzus persicae) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in M. persicae nicotianae, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 UGT transcripts identified in the M. persicae genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341–UGT345, and UGT348–UGT350, with UGT344 containing the most (57). Ten UGTs (UGT330A3, UGT339A2, UGT341A6, UGT342B3, UGT343C3, UGT344D5, UGT344D8, UGT348A3, UGT349A3, and UGT350A3) were highly expressed in M. persicae nicotianae compared to M. persicae sensu stricto. Knockdown of four UGTs (UGT330A3, UGT344D5, UGT348A3, and UGT349A3) significantly increased M. persicae nicotianae sensitivity to nicotine, suggesting that UGT expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible UGTs relevant to nicotine adaptation in tobacco-consuming M. persicae nicotianae, and the findings will facilitate further validation of the roles of these UGTs in nicotine tolerance.
format Online
Article
Text
id pubmed-6695686
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-66956862019-09-05 Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in Myzus persicae Pan, Yiou Xu, Pengjun Zeng, Xiaochun Liu, Xuemei Shang, Qingli Int J Mol Sci Article Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of UGTs and other detoxification enzymes. However, UGT functions in green peach aphid (Myzus persicae) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in M. persicae nicotianae, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 UGT transcripts identified in the M. persicae genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341–UGT345, and UGT348–UGT350, with UGT344 containing the most (57). Ten UGTs (UGT330A3, UGT339A2, UGT341A6, UGT342B3, UGT343C3, UGT344D5, UGT344D8, UGT348A3, UGT349A3, and UGT350A3) were highly expressed in M. persicae nicotianae compared to M. persicae sensu stricto. Knockdown of four UGTs (UGT330A3, UGT344D5, UGT348A3, and UGT349A3) significantly increased M. persicae nicotianae sensitivity to nicotine, suggesting that UGT expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible UGTs relevant to nicotine adaptation in tobacco-consuming M. persicae nicotianae, and the findings will facilitate further validation of the roles of these UGTs in nicotine tolerance. MDPI 2019-07-25 /pmc/articles/PMC6695686/ /pubmed/31349586 http://dx.doi.org/10.3390/ijms20153637 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pan, Yiou
Xu, Pengjun
Zeng, Xiaochun
Liu, Xuemei
Shang, Qingli
Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in Myzus persicae
title Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in Myzus persicae
title_full Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in Myzus persicae
title_fullStr Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in Myzus persicae
title_full_unstemmed Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in Myzus persicae
title_short Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in Myzus persicae
title_sort characterization of udp-glucuronosyltransferases and the potential contribution to nicotine tolerance in myzus persicae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695686/
https://www.ncbi.nlm.nih.gov/pubmed/31349586
http://dx.doi.org/10.3390/ijms20153637
work_keys_str_mv AT panyiou characterizationofudpglucuronosyltransferasesandthepotentialcontributiontonicotinetoleranceinmyzuspersicae
AT xupengjun characterizationofudpglucuronosyltransferasesandthepotentialcontributiontonicotinetoleranceinmyzuspersicae
AT zengxiaochun characterizationofudpglucuronosyltransferasesandthepotentialcontributiontonicotinetoleranceinmyzuspersicae
AT liuxuemei characterizationofudpglucuronosyltransferasesandthepotentialcontributiontonicotinetoleranceinmyzuspersicae
AT shangqingli characterizationofudpglucuronosyltransferasesandthepotentialcontributiontonicotinetoleranceinmyzuspersicae