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A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk

Early life exposure to folate has long lasting effects on development and health. Newborns obtain part of their folate from maternal milk. Studies on health effects of milk folate require rapid, affordable and reliable measurements in large numbers of samples from cohort studies. Recently, a competi...

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Autores principales: Guignard, Laurence, Nembrini, Chiara, Moulin, Julie, Meisser, Karine, Silva-Zolezzi, Irma, Kratzsch, Jürgen, Vogel, Mandy, Kiess, Wieland, Eckhardt, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695834/
https://www.ncbi.nlm.nih.gov/pubmed/31357571
http://dx.doi.org/10.3390/molecules24152730
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author Guignard, Laurence
Nembrini, Chiara
Moulin, Julie
Meisser, Karine
Silva-Zolezzi, Irma
Kratzsch, Jürgen
Vogel, Mandy
Kiess, Wieland
Eckhardt, Erik
author_facet Guignard, Laurence
Nembrini, Chiara
Moulin, Julie
Meisser, Karine
Silva-Zolezzi, Irma
Kratzsch, Jürgen
Vogel, Mandy
Kiess, Wieland
Eckhardt, Erik
author_sort Guignard, Laurence
collection PubMed
description Early life exposure to folate has long lasting effects on development and health. Newborns obtain part of their folate from maternal milk. Studies on health effects of milk folate require rapid, affordable and reliable measurements in large numbers of samples from cohort studies. Recently, a competitive chemiluminescence assay for quantification of folate has become available for automated diagnostic measurement of folate in human serum or plasma. We tested if this method (“FOLA” from Siemens Healthcare) could also be used for human milk. To minimize interference and matrix effects, samples had to be skimmed, diluted seven times with demineralized water, and heated for 5 min at 90 °C. Folate could thus be measured in a linear range between 8.4 and 111.7 nM, with recoveries for the most relevant form, 5-methyltetrahydrofolate (5-MeTHF), of 96%–107%. Results were comparable to those with a recently validated Liquid Chromatography/Mass Spectrometry method (Y = 0.998X − 0.2; R(2) = 0.807). The FOLA method was subsequently used for samples from the LIFE Child cohort in Germany, providing first data of breast milk folate in this country (range: 6.2–100.7 nM). This technique could indeed prove useful for large cohorts with multiple samplings.
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spelling pubmed-66958342019-09-05 A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk Guignard, Laurence Nembrini, Chiara Moulin, Julie Meisser, Karine Silva-Zolezzi, Irma Kratzsch, Jürgen Vogel, Mandy Kiess, Wieland Eckhardt, Erik Molecules Article Early life exposure to folate has long lasting effects on development and health. Newborns obtain part of their folate from maternal milk. Studies on health effects of milk folate require rapid, affordable and reliable measurements in large numbers of samples from cohort studies. Recently, a competitive chemiluminescence assay for quantification of folate has become available for automated diagnostic measurement of folate in human serum or plasma. We tested if this method (“FOLA” from Siemens Healthcare) could also be used for human milk. To minimize interference and matrix effects, samples had to be skimmed, diluted seven times with demineralized water, and heated for 5 min at 90 °C. Folate could thus be measured in a linear range between 8.4 and 111.7 nM, with recoveries for the most relevant form, 5-methyltetrahydrofolate (5-MeTHF), of 96%–107%. Results were comparable to those with a recently validated Liquid Chromatography/Mass Spectrometry method (Y = 0.998X − 0.2; R(2) = 0.807). The FOLA method was subsequently used for samples from the LIFE Child cohort in Germany, providing first data of breast milk folate in this country (range: 6.2–100.7 nM). This technique could indeed prove useful for large cohorts with multiple samplings. MDPI 2019-07-27 /pmc/articles/PMC6695834/ /pubmed/31357571 http://dx.doi.org/10.3390/molecules24152730 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guignard, Laurence
Nembrini, Chiara
Moulin, Julie
Meisser, Karine
Silva-Zolezzi, Irma
Kratzsch, Jürgen
Vogel, Mandy
Kiess, Wieland
Eckhardt, Erik
A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk
title A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk
title_full A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk
title_fullStr A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk
title_full_unstemmed A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk
title_short A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk
title_sort rapid chemiluminescence assay for measurement of folate in small volumes of breast milk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695834/
https://www.ncbi.nlm.nih.gov/pubmed/31357571
http://dx.doi.org/10.3390/molecules24152730
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