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Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana

Arabidopsis roots accumulate a complex mixture of dolichols composed of three families, (i.e., short-, medium- and long-chain dolichols), but until now none of the cis-prenyltransferases (CPTs) predicted in the Arabidopsis genome has been considered responsible for their synthesis. In this report, u...

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Autores principales: Surowiecki, Przemyslaw, Onysk, Agnieszka, Manko, Katarzyna, Swiezewska, Ewa, Surmacz, Liliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695881/
https://www.ncbi.nlm.nih.gov/pubmed/31370240
http://dx.doi.org/10.3390/molecules24152789
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author Surowiecki, Przemyslaw
Onysk, Agnieszka
Manko, Katarzyna
Swiezewska, Ewa
Surmacz, Liliana
author_facet Surowiecki, Przemyslaw
Onysk, Agnieszka
Manko, Katarzyna
Swiezewska, Ewa
Surmacz, Liliana
author_sort Surowiecki, Przemyslaw
collection PubMed
description Arabidopsis roots accumulate a complex mixture of dolichols composed of three families, (i.e., short-, medium- and long-chain dolichols), but until now none of the cis-prenyltransferases (CPTs) predicted in the Arabidopsis genome has been considered responsible for their synthesis. In this report, using homo- and heterologous (yeast and tobacco) models, we have characterized the AtCPT1 gene (At2g23410) which encodes a CPT responsible for the formation of long-chain dolichols, Dol-18 to -23, with Dol-21 dominating, in Arabidopsis. The content of these dolichols was significantly reduced in AtCPT1 T-DNA insertion mutant lines and highly increased in AtCPT1-overexpressing plants. Similar to the majority of eukaryotic CPTs, AtCPT1 is localized to the endoplasmic reticulum (ER). Functional complementation tests using yeast rer2Δ or srt1Δ mutants devoid of medium- or long-chain dolichols, respectively, confirmed that this enzyme synthesizes long-chain dolichols, although the dolichol chains thus formed are somewhat shorter than those synthesized in planta. Moreover, AtCPT1 acts as a homomeric CPT and does not need LEW1 for its activity. AtCPT1 is the first plant CPT producing long-chain polyisoprenoids that does not form a complex with the NgBR/NUS1 homologue.
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spelling pubmed-66958812019-09-05 Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana Surowiecki, Przemyslaw Onysk, Agnieszka Manko, Katarzyna Swiezewska, Ewa Surmacz, Liliana Molecules Article Arabidopsis roots accumulate a complex mixture of dolichols composed of three families, (i.e., short-, medium- and long-chain dolichols), but until now none of the cis-prenyltransferases (CPTs) predicted in the Arabidopsis genome has been considered responsible for their synthesis. In this report, using homo- and heterologous (yeast and tobacco) models, we have characterized the AtCPT1 gene (At2g23410) which encodes a CPT responsible for the formation of long-chain dolichols, Dol-18 to -23, with Dol-21 dominating, in Arabidopsis. The content of these dolichols was significantly reduced in AtCPT1 T-DNA insertion mutant lines and highly increased in AtCPT1-overexpressing plants. Similar to the majority of eukaryotic CPTs, AtCPT1 is localized to the endoplasmic reticulum (ER). Functional complementation tests using yeast rer2Δ or srt1Δ mutants devoid of medium- or long-chain dolichols, respectively, confirmed that this enzyme synthesizes long-chain dolichols, although the dolichol chains thus formed are somewhat shorter than those synthesized in planta. Moreover, AtCPT1 acts as a homomeric CPT and does not need LEW1 for its activity. AtCPT1 is the first plant CPT producing long-chain polyisoprenoids that does not form a complex with the NgBR/NUS1 homologue. MDPI 2019-07-31 /pmc/articles/PMC6695881/ /pubmed/31370240 http://dx.doi.org/10.3390/molecules24152789 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Surowiecki, Przemyslaw
Onysk, Agnieszka
Manko, Katarzyna
Swiezewska, Ewa
Surmacz, Liliana
Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana
title Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana
title_full Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana
title_fullStr Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana
title_full_unstemmed Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana
title_short Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana
title_sort long-chain polyisoprenoids are synthesized by atcpt1 in arabidopsis thaliana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695881/
https://www.ncbi.nlm.nih.gov/pubmed/31370240
http://dx.doi.org/10.3390/molecules24152789
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