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Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana
Arabidopsis roots accumulate a complex mixture of dolichols composed of three families, (i.e., short-, medium- and long-chain dolichols), but until now none of the cis-prenyltransferases (CPTs) predicted in the Arabidopsis genome has been considered responsible for their synthesis. In this report, u...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695881/ https://www.ncbi.nlm.nih.gov/pubmed/31370240 http://dx.doi.org/10.3390/molecules24152789 |
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author | Surowiecki, Przemyslaw Onysk, Agnieszka Manko, Katarzyna Swiezewska, Ewa Surmacz, Liliana |
author_facet | Surowiecki, Przemyslaw Onysk, Agnieszka Manko, Katarzyna Swiezewska, Ewa Surmacz, Liliana |
author_sort | Surowiecki, Przemyslaw |
collection | PubMed |
description | Arabidopsis roots accumulate a complex mixture of dolichols composed of three families, (i.e., short-, medium- and long-chain dolichols), but until now none of the cis-prenyltransferases (CPTs) predicted in the Arabidopsis genome has been considered responsible for their synthesis. In this report, using homo- and heterologous (yeast and tobacco) models, we have characterized the AtCPT1 gene (At2g23410) which encodes a CPT responsible for the formation of long-chain dolichols, Dol-18 to -23, with Dol-21 dominating, in Arabidopsis. The content of these dolichols was significantly reduced in AtCPT1 T-DNA insertion mutant lines and highly increased in AtCPT1-overexpressing plants. Similar to the majority of eukaryotic CPTs, AtCPT1 is localized to the endoplasmic reticulum (ER). Functional complementation tests using yeast rer2Δ or srt1Δ mutants devoid of medium- or long-chain dolichols, respectively, confirmed that this enzyme synthesizes long-chain dolichols, although the dolichol chains thus formed are somewhat shorter than those synthesized in planta. Moreover, AtCPT1 acts as a homomeric CPT and does not need LEW1 for its activity. AtCPT1 is the first plant CPT producing long-chain polyisoprenoids that does not form a complex with the NgBR/NUS1 homologue. |
format | Online Article Text |
id | pubmed-6695881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66958812019-09-05 Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana Surowiecki, Przemyslaw Onysk, Agnieszka Manko, Katarzyna Swiezewska, Ewa Surmacz, Liliana Molecules Article Arabidopsis roots accumulate a complex mixture of dolichols composed of three families, (i.e., short-, medium- and long-chain dolichols), but until now none of the cis-prenyltransferases (CPTs) predicted in the Arabidopsis genome has been considered responsible for their synthesis. In this report, using homo- and heterologous (yeast and tobacco) models, we have characterized the AtCPT1 gene (At2g23410) which encodes a CPT responsible for the formation of long-chain dolichols, Dol-18 to -23, with Dol-21 dominating, in Arabidopsis. The content of these dolichols was significantly reduced in AtCPT1 T-DNA insertion mutant lines and highly increased in AtCPT1-overexpressing plants. Similar to the majority of eukaryotic CPTs, AtCPT1 is localized to the endoplasmic reticulum (ER). Functional complementation tests using yeast rer2Δ or srt1Δ mutants devoid of medium- or long-chain dolichols, respectively, confirmed that this enzyme synthesizes long-chain dolichols, although the dolichol chains thus formed are somewhat shorter than those synthesized in planta. Moreover, AtCPT1 acts as a homomeric CPT and does not need LEW1 for its activity. AtCPT1 is the first plant CPT producing long-chain polyisoprenoids that does not form a complex with the NgBR/NUS1 homologue. MDPI 2019-07-31 /pmc/articles/PMC6695881/ /pubmed/31370240 http://dx.doi.org/10.3390/molecules24152789 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Surowiecki, Przemyslaw Onysk, Agnieszka Manko, Katarzyna Swiezewska, Ewa Surmacz, Liliana Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana |
title | Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana |
title_full | Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana |
title_fullStr | Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana |
title_full_unstemmed | Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana |
title_short | Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana |
title_sort | long-chain polyisoprenoids are synthesized by atcpt1 in arabidopsis thaliana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6695881/ https://www.ncbi.nlm.nih.gov/pubmed/31370240 http://dx.doi.org/10.3390/molecules24152789 |
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