Affinity Immobilization of a Bacterial Prolidase onto Metal-Ion-Chelated Magnetic Nanoparticles for the Hydrolysis of Organophosphorus Compounds

In this study, silica-coated magnetic nanoparticles (SiMNPs) with isocyanatopropyltriethoxysilane as a metal-chelating ligand were prepared for the immobilization of His(6)-tagged Escherichia coli prolidase (His(6)-EcPepQ). Under one-hour coupling, the enzyme-loading capacity for the Ni(2+)-function...

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Detalles Bibliográficos
Autores principales: Wang, Tzu-Fan, Lo, Huei-Fen, Chi, Meng-Chun, Lai, Kuan-Ling, Lin, Min-Guan, Lin, Long-Liu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696040/
https://www.ncbi.nlm.nih.gov/pubmed/31344929
http://dx.doi.org/10.3390/ijms20153625

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