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Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco

Cadmium (Cd) is an environmental pollutant that potentially threatens human health worldwide. Developing approaches for efficiently treating environmental Cd is a priority. Selenium (Se) plays important role in the protection of plants against various abiotic stresses, including heavy metals. Previo...

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Autores principales: Luo, Yong, Wei, Yuewei, Sun, Shuguang, Wang, Jian, Wang, Weifeng, Han, Dan, Shao, Huifang, Jia, Hongfang, Fu, Yunpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696094/
https://www.ncbi.nlm.nih.gov/pubmed/31374993
http://dx.doi.org/10.3390/ijms20153772
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author Luo, Yong
Wei, Yuewei
Sun, Shuguang
Wang, Jian
Wang, Weifeng
Han, Dan
Shao, Huifang
Jia, Hongfang
Fu, Yunpeng
author_facet Luo, Yong
Wei, Yuewei
Sun, Shuguang
Wang, Jian
Wang, Weifeng
Han, Dan
Shao, Huifang
Jia, Hongfang
Fu, Yunpeng
author_sort Luo, Yong
collection PubMed
description Cadmium (Cd) is an environmental pollutant that potentially threatens human health worldwide. Developing approaches for efficiently treating environmental Cd is a priority. Selenium (Se) plays important role in the protection of plants against various abiotic stresses, including heavy metals. Previous research has shown that Se can alleviate Cd toxicity, but the molecular mechanism is still not clear. In this study, we explore the function of auxin and phosphate (P) in tobacco (Nicotiana tabacum), with particular focus on their interaction with Se and Cd. Under Cd stress conditions, low Se (10 μM) significantly increased the biomass and antioxidant capacity of tobacco plants and reduced uptake of Cd. We also measured the auxin concentration and expression of auxin-relative genes in tobacco and found that plants treated with low Se (10 μM) had higher auxin concentrations at different Cd supply levels (0 μM, 20 μM, 50 μM) compared with no Se treatment, probably due to increased expression of auxin synthesis genes and auxin efflux carriers. Overexpression of a high affinity phosphate transporter NtPT2 enhanced the tolerance of tobacco to Cd stress, possibly by increasing the total P and Se content and decreasing Cd accumulation compared to that in the wild type (WT). Our results show that there is an interactive mechanism among P, Se, Cd, and auxin that affects plant growth and may provide a new approach for relieving Cd toxicity in plants.
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spelling pubmed-66960942019-09-05 Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco Luo, Yong Wei, Yuewei Sun, Shuguang Wang, Jian Wang, Weifeng Han, Dan Shao, Huifang Jia, Hongfang Fu, Yunpeng Int J Mol Sci Article Cadmium (Cd) is an environmental pollutant that potentially threatens human health worldwide. Developing approaches for efficiently treating environmental Cd is a priority. Selenium (Se) plays important role in the protection of plants against various abiotic stresses, including heavy metals. Previous research has shown that Se can alleviate Cd toxicity, but the molecular mechanism is still not clear. In this study, we explore the function of auxin and phosphate (P) in tobacco (Nicotiana tabacum), with particular focus on their interaction with Se and Cd. Under Cd stress conditions, low Se (10 μM) significantly increased the biomass and antioxidant capacity of tobacco plants and reduced uptake of Cd. We also measured the auxin concentration and expression of auxin-relative genes in tobacco and found that plants treated with low Se (10 μM) had higher auxin concentrations at different Cd supply levels (0 μM, 20 μM, 50 μM) compared with no Se treatment, probably due to increased expression of auxin synthesis genes and auxin efflux carriers. Overexpression of a high affinity phosphate transporter NtPT2 enhanced the tolerance of tobacco to Cd stress, possibly by increasing the total P and Se content and decreasing Cd accumulation compared to that in the wild type (WT). Our results show that there is an interactive mechanism among P, Se, Cd, and auxin that affects plant growth and may provide a new approach for relieving Cd toxicity in plants. MDPI 2019-08-01 /pmc/articles/PMC6696094/ /pubmed/31374993 http://dx.doi.org/10.3390/ijms20153772 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luo, Yong
Wei, Yuewei
Sun, Shuguang
Wang, Jian
Wang, Weifeng
Han, Dan
Shao, Huifang
Jia, Hongfang
Fu, Yunpeng
Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco
title Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco
title_full Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco
title_fullStr Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco
title_full_unstemmed Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco
title_short Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco
title_sort selenium modulates the level of auxin to alleviate the toxicity of cadmium in tobacco
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696094/
https://www.ncbi.nlm.nih.gov/pubmed/31374993
http://dx.doi.org/10.3390/ijms20153772
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