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Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines

Studies conducted over the last 40 years have demonstrated that the water output from dental unit waterlines (DUWLs) is often contaminated with high densities of microorganisms. It has been monitored the microbiological quality of the water in 30 public dental facilities in northern Italy in order t...

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Autores principales: Spagnolo, Anna Maria, Sartini, Marina, Di Cave, David, Casini, Beatrice, Tuvo, Benedetta, Cristina, Maria Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696308/
https://www.ncbi.nlm.nih.gov/pubmed/31344972
http://dx.doi.org/10.3390/ijerph16152648
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author Spagnolo, Anna Maria
Sartini, Marina
Di Cave, David
Casini, Beatrice
Tuvo, Benedetta
Cristina, Maria Luisa
author_facet Spagnolo, Anna Maria
Sartini, Marina
Di Cave, David
Casini, Beatrice
Tuvo, Benedetta
Cristina, Maria Luisa
author_sort Spagnolo, Anna Maria
collection PubMed
description Studies conducted over the last 40 years have demonstrated that the water output from dental unit waterlines (DUWLs) is often contaminated with high densities of microorganisms. It has been monitored the microbiological quality of the water in 30 public dental facilities in northern Italy in order to assess the health risk for patients and dental staff. In each facility, samples of water both from taps and from DUWLs were analyzed in order to evaluate heterotrophic plate counts (HPCs) at 22 °C and 36 °C, and to detect coliform bacteria, Pseudomonas aeruginosa, Legionella pneumophila and amoebae. In 100% of the samples taken from the DUWLs, the concentration of HPCs was above the threshold as determined by the Ministère de la Santé et des Solidarités (2007). The concentration of P. aeruginosa was greater than the indicated threshold in 16.67% of the hand-pieces analyzed. A total of 78.33% of samples were contaminated by L. pneumophila, while in the samples taken from the DUWLs alone, this percentage rose to 86.67%. Amoebae were detected in 60% of the samples taken from hand-pieces; all belonging to the species V. vermiformis. This study documented the presence of various microorganisms, including Legionella spp., at considerably higher concentrations in water samples from DUWLs than in samples of tap water in the same facilities, confirming the role of the internal DUWLs in increasing microbial contamination, especially in the absence of proper management of waterborne health risks.
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spelling pubmed-66963082019-09-05 Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines Spagnolo, Anna Maria Sartini, Marina Di Cave, David Casini, Beatrice Tuvo, Benedetta Cristina, Maria Luisa Int J Environ Res Public Health Article Studies conducted over the last 40 years have demonstrated that the water output from dental unit waterlines (DUWLs) is often contaminated with high densities of microorganisms. It has been monitored the microbiological quality of the water in 30 public dental facilities in northern Italy in order to assess the health risk for patients and dental staff. In each facility, samples of water both from taps and from DUWLs were analyzed in order to evaluate heterotrophic plate counts (HPCs) at 22 °C and 36 °C, and to detect coliform bacteria, Pseudomonas aeruginosa, Legionella pneumophila and amoebae. In 100% of the samples taken from the DUWLs, the concentration of HPCs was above the threshold as determined by the Ministère de la Santé et des Solidarités (2007). The concentration of P. aeruginosa was greater than the indicated threshold in 16.67% of the hand-pieces analyzed. A total of 78.33% of samples were contaminated by L. pneumophila, while in the samples taken from the DUWLs alone, this percentage rose to 86.67%. Amoebae were detected in 60% of the samples taken from hand-pieces; all belonging to the species V. vermiformis. This study documented the presence of various microorganisms, including Legionella spp., at considerably higher concentrations in water samples from DUWLs than in samples of tap water in the same facilities, confirming the role of the internal DUWLs in increasing microbial contamination, especially in the absence of proper management of waterborne health risks. MDPI 2019-07-24 2019-08 /pmc/articles/PMC6696308/ /pubmed/31344972 http://dx.doi.org/10.3390/ijerph16152648 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Spagnolo, Anna Maria
Sartini, Marina
Di Cave, David
Casini, Beatrice
Tuvo, Benedetta
Cristina, Maria Luisa
Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines
title Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines
title_full Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines
title_fullStr Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines
title_full_unstemmed Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines
title_short Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines
title_sort evaluation of microbiological and free-living protozoa contamination in dental unit waterlines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696308/
https://www.ncbi.nlm.nih.gov/pubmed/31344972
http://dx.doi.org/10.3390/ijerph16152648
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