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Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice
The G-protein coupled cannabinoid receptor 2 (CB2) has been implicated in the regulation of adult neurogenesis in the hippocampus. The contribution of CB2 towards basal levels of proliferation and the number of neural progenitors in the subgranular zone (SGZ) of the dentate gyrus, however, remain un...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696320/ https://www.ncbi.nlm.nih.gov/pubmed/31374821 http://dx.doi.org/10.3390/ijms20153759 |
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author | Mensching, Leonore Djogo, Nevena Keller, Christina Rading, Sebastian Karsak, Meliha |
author_facet | Mensching, Leonore Djogo, Nevena Keller, Christina Rading, Sebastian Karsak, Meliha |
author_sort | Mensching, Leonore |
collection | PubMed |
description | The G-protein coupled cannabinoid receptor 2 (CB2) has been implicated in the regulation of adult neurogenesis in the hippocampus. The contribution of CB2 towards basal levels of proliferation and the number of neural progenitors in the subgranular zone (SGZ) of the dentate gyrus, however, remain unclear. We stained hippocampal brain sections of 16- to 17-week-old wildtype and CB2-deficient mice, for neural progenitor and immature neuron markers doublecortin (DCX) and calretinin (CR) and for the proliferation marker Ki67 and quantified the number of positive cells in the SGZ. The quantification revealed that CB2 deficiency neither altered overall cell proliferation nor the size of the DCX+ or DCX and CR double-positive populations in the SGZ compared to control animals. The results indicate that CB2 might not contribute to basal levels of adult neurogenesis in four-month-old healthy mice. CB2 signaling might be more relevant in conditions where adult neurogenesis is dynamically regulated, such as neuroinflammation. |
format | Online Article Text |
id | pubmed-6696320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66963202019-09-05 Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice Mensching, Leonore Djogo, Nevena Keller, Christina Rading, Sebastian Karsak, Meliha Int J Mol Sci Communication The G-protein coupled cannabinoid receptor 2 (CB2) has been implicated in the regulation of adult neurogenesis in the hippocampus. The contribution of CB2 towards basal levels of proliferation and the number of neural progenitors in the subgranular zone (SGZ) of the dentate gyrus, however, remain unclear. We stained hippocampal brain sections of 16- to 17-week-old wildtype and CB2-deficient mice, for neural progenitor and immature neuron markers doublecortin (DCX) and calretinin (CR) and for the proliferation marker Ki67 and quantified the number of positive cells in the SGZ. The quantification revealed that CB2 deficiency neither altered overall cell proliferation nor the size of the DCX+ or DCX and CR double-positive populations in the SGZ compared to control animals. The results indicate that CB2 might not contribute to basal levels of adult neurogenesis in four-month-old healthy mice. CB2 signaling might be more relevant in conditions where adult neurogenesis is dynamically regulated, such as neuroinflammation. MDPI 2019-08-01 /pmc/articles/PMC6696320/ /pubmed/31374821 http://dx.doi.org/10.3390/ijms20153759 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Mensching, Leonore Djogo, Nevena Keller, Christina Rading, Sebastian Karsak, Meliha Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice |
title | Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice |
title_full | Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice |
title_fullStr | Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice |
title_full_unstemmed | Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice |
title_short | Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice |
title_sort | stable adult hippocampal neurogenesis in cannabinoid receptor cb2 deficient mice |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696320/ https://www.ncbi.nlm.nih.gov/pubmed/31374821 http://dx.doi.org/10.3390/ijms20153759 |
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