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Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice

The G-protein coupled cannabinoid receptor 2 (CB2) has been implicated in the regulation of adult neurogenesis in the hippocampus. The contribution of CB2 towards basal levels of proliferation and the number of neural progenitors in the subgranular zone (SGZ) of the dentate gyrus, however, remain un...

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Detalles Bibliográficos
Autores principales: Mensching, Leonore, Djogo, Nevena, Keller, Christina, Rading, Sebastian, Karsak, Meliha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696320/
https://www.ncbi.nlm.nih.gov/pubmed/31374821
http://dx.doi.org/10.3390/ijms20153759
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author Mensching, Leonore
Djogo, Nevena
Keller, Christina
Rading, Sebastian
Karsak, Meliha
author_facet Mensching, Leonore
Djogo, Nevena
Keller, Christina
Rading, Sebastian
Karsak, Meliha
author_sort Mensching, Leonore
collection PubMed
description The G-protein coupled cannabinoid receptor 2 (CB2) has been implicated in the regulation of adult neurogenesis in the hippocampus. The contribution of CB2 towards basal levels of proliferation and the number of neural progenitors in the subgranular zone (SGZ) of the dentate gyrus, however, remain unclear. We stained hippocampal brain sections of 16- to 17-week-old wildtype and CB2-deficient mice, for neural progenitor and immature neuron markers doublecortin (DCX) and calretinin (CR) and for the proliferation marker Ki67 and quantified the number of positive cells in the SGZ. The quantification revealed that CB2 deficiency neither altered overall cell proliferation nor the size of the DCX+ or DCX and CR double-positive populations in the SGZ compared to control animals. The results indicate that CB2 might not contribute to basal levels of adult neurogenesis in four-month-old healthy mice. CB2 signaling might be more relevant in conditions where adult neurogenesis is dynamically regulated, such as neuroinflammation.
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spelling pubmed-66963202019-09-05 Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice Mensching, Leonore Djogo, Nevena Keller, Christina Rading, Sebastian Karsak, Meliha Int J Mol Sci Communication The G-protein coupled cannabinoid receptor 2 (CB2) has been implicated in the regulation of adult neurogenesis in the hippocampus. The contribution of CB2 towards basal levels of proliferation and the number of neural progenitors in the subgranular zone (SGZ) of the dentate gyrus, however, remain unclear. We stained hippocampal brain sections of 16- to 17-week-old wildtype and CB2-deficient mice, for neural progenitor and immature neuron markers doublecortin (DCX) and calretinin (CR) and for the proliferation marker Ki67 and quantified the number of positive cells in the SGZ. The quantification revealed that CB2 deficiency neither altered overall cell proliferation nor the size of the DCX+ or DCX and CR double-positive populations in the SGZ compared to control animals. The results indicate that CB2 might not contribute to basal levels of adult neurogenesis in four-month-old healthy mice. CB2 signaling might be more relevant in conditions where adult neurogenesis is dynamically regulated, such as neuroinflammation. MDPI 2019-08-01 /pmc/articles/PMC6696320/ /pubmed/31374821 http://dx.doi.org/10.3390/ijms20153759 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Mensching, Leonore
Djogo, Nevena
Keller, Christina
Rading, Sebastian
Karsak, Meliha
Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice
title Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice
title_full Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice
title_fullStr Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice
title_full_unstemmed Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice
title_short Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice
title_sort stable adult hippocampal neurogenesis in cannabinoid receptor cb2 deficient mice
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696320/
https://www.ncbi.nlm.nih.gov/pubmed/31374821
http://dx.doi.org/10.3390/ijms20153759
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