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Peripheral blood mononuclear cells extracts VEGF protein levels and VEGF mRNA: Associations with inflammatory molecules in a healthy population

BACKGROUND: Vascular endothelial growth factor (VEGF) is a signal protein, implicated in various physiological and pathophysiological processes together with other common inflammatory biomarkers. However, their associations have not yet been fully elucidated. In the present study, we investigated as...

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Autores principales: Gorenjak, Vesna, Vance, Dwaine R., Petrelis, Alexandros M., Stathopoulou, Maria G., Dadé, Sébastien, Shamieh, Said El, Murray, Helena, Masson, Christine, Lamont, John, Fitzgerald, Peter, Visvikis-Siest, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697334/
https://www.ncbi.nlm.nih.gov/pubmed/31419243
http://dx.doi.org/10.1371/journal.pone.0220902
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author Gorenjak, Vesna
Vance, Dwaine R.
Petrelis, Alexandros M.
Stathopoulou, Maria G.
Dadé, Sébastien
Shamieh, Said El
Murray, Helena
Masson, Christine
Lamont, John
Fitzgerald, Peter
Visvikis-Siest, Sophie
author_facet Gorenjak, Vesna
Vance, Dwaine R.
Petrelis, Alexandros M.
Stathopoulou, Maria G.
Dadé, Sébastien
Shamieh, Said El
Murray, Helena
Masson, Christine
Lamont, John
Fitzgerald, Peter
Visvikis-Siest, Sophie
author_sort Gorenjak, Vesna
collection PubMed
description BACKGROUND: Vascular endothelial growth factor (VEGF) is a signal protein, implicated in various physiological and pathophysiological processes together with other common inflammatory biomarkers. However, their associations have not yet been fully elucidated. In the present study, we investigated associations between VEGF and four specific VEGF mRNA isoforms with levels of 11 inflammation molecules, derived from peripheral blood mononuclear cells (PBMCs) extracts. METHODS: Healthy participants from the STANISLAS Family Study (n = 285) were included. Levels of VEGF (four mRNA isoforms and protein levels) and inflammatory molecules (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, INF-γ, TNF-α, MCP-1, EGF) were measured in PBMCs extracts. Multiple regression analyses were performed, adjusted for age and gender. RESULTS: The analyses revealed significant associations between VEGF protein levels and levels of IL-4 (β = 0.028, P = 0.013), MCP-1 (β = 0.015, P<0.0001) and EGF (β = 0.017, P<0.0001). Furthermore, mRNA isoform VEGF(165) was associated with MCP-1 and IL-1α (P = 0.002 and P = 0.008, respectively); and mRNA isoform VEGF(189) was associated with IL-4 and IL-6 (P = 0.019 and P = 0.034, respectively). CONCLUSIONS: To our knowledge, the present study represents the first investigation that successfully demonstrates links between VEGF protein levels and inflammatory molecules levels derived from PBMCs extracts and identifies associations between specific VEGF mRNA isoforms and inflammatory molecules. IMPACT: These findings provide novel insights that may assist in the development of new tissue and mRNA isoform specific measurements of VEGF levels, which may positively contribute to predicting the risk of common complex diseases and response of currently used anti-VEGF agents, and developing of novel targeted therapies for VEGF-related pathophysiology.
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spelling pubmed-66973342019-08-30 Peripheral blood mononuclear cells extracts VEGF protein levels and VEGF mRNA: Associations with inflammatory molecules in a healthy population Gorenjak, Vesna Vance, Dwaine R. Petrelis, Alexandros M. Stathopoulou, Maria G. Dadé, Sébastien Shamieh, Said El Murray, Helena Masson, Christine Lamont, John Fitzgerald, Peter Visvikis-Siest, Sophie PLoS One Research Article BACKGROUND: Vascular endothelial growth factor (VEGF) is a signal protein, implicated in various physiological and pathophysiological processes together with other common inflammatory biomarkers. However, their associations have not yet been fully elucidated. In the present study, we investigated associations between VEGF and four specific VEGF mRNA isoforms with levels of 11 inflammation molecules, derived from peripheral blood mononuclear cells (PBMCs) extracts. METHODS: Healthy participants from the STANISLAS Family Study (n = 285) were included. Levels of VEGF (four mRNA isoforms and protein levels) and inflammatory molecules (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, INF-γ, TNF-α, MCP-1, EGF) were measured in PBMCs extracts. Multiple regression analyses were performed, adjusted for age and gender. RESULTS: The analyses revealed significant associations between VEGF protein levels and levels of IL-4 (β = 0.028, P = 0.013), MCP-1 (β = 0.015, P<0.0001) and EGF (β = 0.017, P<0.0001). Furthermore, mRNA isoform VEGF(165) was associated with MCP-1 and IL-1α (P = 0.002 and P = 0.008, respectively); and mRNA isoform VEGF(189) was associated with IL-4 and IL-6 (P = 0.019 and P = 0.034, respectively). CONCLUSIONS: To our knowledge, the present study represents the first investigation that successfully demonstrates links between VEGF protein levels and inflammatory molecules levels derived from PBMCs extracts and identifies associations between specific VEGF mRNA isoforms and inflammatory molecules. IMPACT: These findings provide novel insights that may assist in the development of new tissue and mRNA isoform specific measurements of VEGF levels, which may positively contribute to predicting the risk of common complex diseases and response of currently used anti-VEGF agents, and developing of novel targeted therapies for VEGF-related pathophysiology. Public Library of Science 2019-08-16 /pmc/articles/PMC6697334/ /pubmed/31419243 http://dx.doi.org/10.1371/journal.pone.0220902 Text en © 2019 Gorenjak et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gorenjak, Vesna
Vance, Dwaine R.
Petrelis, Alexandros M.
Stathopoulou, Maria G.
Dadé, Sébastien
Shamieh, Said El
Murray, Helena
Masson, Christine
Lamont, John
Fitzgerald, Peter
Visvikis-Siest, Sophie
Peripheral blood mononuclear cells extracts VEGF protein levels and VEGF mRNA: Associations with inflammatory molecules in a healthy population
title Peripheral blood mononuclear cells extracts VEGF protein levels and VEGF mRNA: Associations with inflammatory molecules in a healthy population
title_full Peripheral blood mononuclear cells extracts VEGF protein levels and VEGF mRNA: Associations with inflammatory molecules in a healthy population
title_fullStr Peripheral blood mononuclear cells extracts VEGF protein levels and VEGF mRNA: Associations with inflammatory molecules in a healthy population
title_full_unstemmed Peripheral blood mononuclear cells extracts VEGF protein levels and VEGF mRNA: Associations with inflammatory molecules in a healthy population
title_short Peripheral blood mononuclear cells extracts VEGF protein levels and VEGF mRNA: Associations with inflammatory molecules in a healthy population
title_sort peripheral blood mononuclear cells extracts vegf protein levels and vegf mrna: associations with inflammatory molecules in a healthy population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697334/
https://www.ncbi.nlm.nih.gov/pubmed/31419243
http://dx.doi.org/10.1371/journal.pone.0220902
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