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Overexpression of the Rieske FeS protein of the Cytochrome b(6)f complex increases C(4) photosynthesis in Setaria viridis
C(4) photosynthesis is characterised by a CO(2) concentrating mechanism that operates between mesophyll and bundle sheath cells increasing CO(2) partial pressure at the site of Rubisco and photosynthetic efficiency. Electron transport chains in both cell types supply ATP and NADPH for C(4) photosynt...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697696/ https://www.ncbi.nlm.nih.gov/pubmed/31453378 http://dx.doi.org/10.1038/s42003-019-0561-9 |
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author | Ermakova, Maria Lopez-Calcagno, Patricia E. Raines, Christine A. Furbank, Robert T. von Caemmerer, Susanne |
author_facet | Ermakova, Maria Lopez-Calcagno, Patricia E. Raines, Christine A. Furbank, Robert T. von Caemmerer, Susanne |
author_sort | Ermakova, Maria |
collection | PubMed |
description | C(4) photosynthesis is characterised by a CO(2) concentrating mechanism that operates between mesophyll and bundle sheath cells increasing CO(2) partial pressure at the site of Rubisco and photosynthetic efficiency. Electron transport chains in both cell types supply ATP and NADPH for C(4) photosynthesis. Cytochrome b(6)f is a key control point of electron transport in C(3) plants. To study whether C(4) photosynthesis is limited by electron transport we constitutively overexpressed the Rieske FeS subunit in Setaria viridis. This resulted in a higher Cytochrome b(6)f content in mesophyll and bundle sheath cells without marked changes in the abundances of other photosynthetic proteins. Rieske overexpression plants showed better light conversion efficiency in both Photosystems and could generate higher proton-motive force across the thylakoid membrane underpinning an increase in CO(2) assimilation rate at ambient and saturating CO(2) and high light. Our results demonstrate that removing electron transport limitations can increase C(4) photosynthesis. |
format | Online Article Text |
id | pubmed-6697696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66976962019-08-26 Overexpression of the Rieske FeS protein of the Cytochrome b(6)f complex increases C(4) photosynthesis in Setaria viridis Ermakova, Maria Lopez-Calcagno, Patricia E. Raines, Christine A. Furbank, Robert T. von Caemmerer, Susanne Commun Biol Article C(4) photosynthesis is characterised by a CO(2) concentrating mechanism that operates between mesophyll and bundle sheath cells increasing CO(2) partial pressure at the site of Rubisco and photosynthetic efficiency. Electron transport chains in both cell types supply ATP and NADPH for C(4) photosynthesis. Cytochrome b(6)f is a key control point of electron transport in C(3) plants. To study whether C(4) photosynthesis is limited by electron transport we constitutively overexpressed the Rieske FeS subunit in Setaria viridis. This resulted in a higher Cytochrome b(6)f content in mesophyll and bundle sheath cells without marked changes in the abundances of other photosynthetic proteins. Rieske overexpression plants showed better light conversion efficiency in both Photosystems and could generate higher proton-motive force across the thylakoid membrane underpinning an increase in CO(2) assimilation rate at ambient and saturating CO(2) and high light. Our results demonstrate that removing electron transport limitations can increase C(4) photosynthesis. Nature Publishing Group UK 2019-08-16 /pmc/articles/PMC6697696/ /pubmed/31453378 http://dx.doi.org/10.1038/s42003-019-0561-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ermakova, Maria Lopez-Calcagno, Patricia E. Raines, Christine A. Furbank, Robert T. von Caemmerer, Susanne Overexpression of the Rieske FeS protein of the Cytochrome b(6)f complex increases C(4) photosynthesis in Setaria viridis |
title | Overexpression of the Rieske FeS protein of the Cytochrome b(6)f complex increases C(4) photosynthesis in Setaria viridis |
title_full | Overexpression of the Rieske FeS protein of the Cytochrome b(6)f complex increases C(4) photosynthesis in Setaria viridis |
title_fullStr | Overexpression of the Rieske FeS protein of the Cytochrome b(6)f complex increases C(4) photosynthesis in Setaria viridis |
title_full_unstemmed | Overexpression of the Rieske FeS protein of the Cytochrome b(6)f complex increases C(4) photosynthesis in Setaria viridis |
title_short | Overexpression of the Rieske FeS protein of the Cytochrome b(6)f complex increases C(4) photosynthesis in Setaria viridis |
title_sort | overexpression of the rieske fes protein of the cytochrome b(6)f complex increases c(4) photosynthesis in setaria viridis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697696/ https://www.ncbi.nlm.nih.gov/pubmed/31453378 http://dx.doi.org/10.1038/s42003-019-0561-9 |
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