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Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators

Despite the crucial physiological processes governed by neurons in the hypothalamic arcuate nucleus (ARC), such as growth, reproduction and energy homeostasis, the developmental pathways and regulators for ARC neurons remain understudied. Our single cell RNA-seq analyses of mouse embryonic ARC revea...

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Autores principales: Huisman, Christian, Cho, Hyeyoung, Brock, Olivier, Lim, Su Jeong, Youn, Sung Min, Park, Younjung, Kim, Sangsoo, Lee, Soo-Kyung, Delogu, Alessio, Lee, Jae W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697706/
https://www.ncbi.nlm.nih.gov/pubmed/31420539
http://dx.doi.org/10.1038/s41467-019-11667-y
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author Huisman, Christian
Cho, Hyeyoung
Brock, Olivier
Lim, Su Jeong
Youn, Sung Min
Park, Younjung
Kim, Sangsoo
Lee, Soo-Kyung
Delogu, Alessio
Lee, Jae W.
author_facet Huisman, Christian
Cho, Hyeyoung
Brock, Olivier
Lim, Su Jeong
Youn, Sung Min
Park, Younjung
Kim, Sangsoo
Lee, Soo-Kyung
Delogu, Alessio
Lee, Jae W.
author_sort Huisman, Christian
collection PubMed
description Despite the crucial physiological processes governed by neurons in the hypothalamic arcuate nucleus (ARC), such as growth, reproduction and energy homeostasis, the developmental pathways and regulators for ARC neurons remain understudied. Our single cell RNA-seq analyses of mouse embryonic ARC revealed many cell type-specific markers for developing ARC neurons. These markers include transcription factors whose expression is enriched in specific neuronal types and often depleted in other closely-related neuronal types, raising the possibility that these transcription factors play important roles in the fate commitment or differentiation of specific ARC neuronal types. We validated this idea with the two transcription factors, Foxp2 enriched for Ghrh-neurons and Sox14 enriched for Kisspeptin-neurons, using Foxp2- and Sox14-deficient mouse models. Taken together, our single cell transcriptome analyses for the developing ARC uncovered a panel of transcription factors that are likely to form a gene regulatory network to orchestrate fate specification and differentiation of ARC neurons.
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spelling pubmed-66977062019-08-19 Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators Huisman, Christian Cho, Hyeyoung Brock, Olivier Lim, Su Jeong Youn, Sung Min Park, Younjung Kim, Sangsoo Lee, Soo-Kyung Delogu, Alessio Lee, Jae W. Nat Commun Article Despite the crucial physiological processes governed by neurons in the hypothalamic arcuate nucleus (ARC), such as growth, reproduction and energy homeostasis, the developmental pathways and regulators for ARC neurons remain understudied. Our single cell RNA-seq analyses of mouse embryonic ARC revealed many cell type-specific markers for developing ARC neurons. These markers include transcription factors whose expression is enriched in specific neuronal types and often depleted in other closely-related neuronal types, raising the possibility that these transcription factors play important roles in the fate commitment or differentiation of specific ARC neuronal types. We validated this idea with the two transcription factors, Foxp2 enriched for Ghrh-neurons and Sox14 enriched for Kisspeptin-neurons, using Foxp2- and Sox14-deficient mouse models. Taken together, our single cell transcriptome analyses for the developing ARC uncovered a panel of transcription factors that are likely to form a gene regulatory network to orchestrate fate specification and differentiation of ARC neurons. Nature Publishing Group UK 2019-08-16 /pmc/articles/PMC6697706/ /pubmed/31420539 http://dx.doi.org/10.1038/s41467-019-11667-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Huisman, Christian
Cho, Hyeyoung
Brock, Olivier
Lim, Su Jeong
Youn, Sung Min
Park, Younjung
Kim, Sangsoo
Lee, Soo-Kyung
Delogu, Alessio
Lee, Jae W.
Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators
title Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators
title_full Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators
title_fullStr Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators
title_full_unstemmed Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators
title_short Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators
title_sort single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697706/
https://www.ncbi.nlm.nih.gov/pubmed/31420539
http://dx.doi.org/10.1038/s41467-019-11667-y
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