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Optimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus
BACKGROUND: The extracellular xylanase secreted by microorganisms is a hydrolytic enzyme, which arbitrarily cleaves the β-1, 4 backbone of the polysaccharide xylan; an enzyme used in the food processing, bio-pulping and bio-bleaching. The commercial production of the xylanase is limited because of a...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Institute of Genetic Engineering and Biotechnology
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697833/ https://www.ncbi.nlm.nih.gov/pubmed/31457031 http://dx.doi.org/10.21859/ijb.1658 |
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author | Kundu, Aditi Majumdar, Bijan |
author_facet | Kundu, Aditi Majumdar, Bijan |
author_sort | Kundu, Aditi |
collection | PubMed |
description | BACKGROUND: The extracellular xylanase secreted by microorganisms is a hydrolytic enzyme, which arbitrarily cleaves the β-1, 4 backbone of the polysaccharide xylan; an enzyme used in the food processing, bio-pulping and bio-bleaching. The commercial production of the xylanase is limited because of a higher cost involvement, which can be overcome by the cost-effective production of the xylanase through immobilization of the microbial cell by the non-toxic substances. OBJECTIVES: In this work, the optimization of the extra-cellular cellulase free xylanase production by the immobilized cell of the Bacillus pumilus IMAU80221 strain using Ca-alginate beads along with standardization of the various parameters for a higher xylanase production were studied. MATERIALS AND METHODS: Following to sterilization, the Na-alginate solution was mixed with the bacterial suspension of the Bacillus pumilus IMAU80221 and was added drop by drop into the 1 M calcium chloride solution for 1 h for obtaining a uniform sized polymeric bead of the Ca-alginate. For xylanase production, the Ca-alginate beads were then transferred into 100 mL Erlenmeyer flasks with 20 mL of the culture medium containing (w/v) 0.02% NaCl, 0.02% MgSO(4), 0.04% (KH(4))(2)PO(4), 0.1% peptone, and 0.5% xylan and incubated at 34 °C in an incubator shaker (150 rpm) for 24 h. The resultant supernatant (crude enzyme) was used for enzyme assay. RESULTS: The maximum xylanase production by the free cell (1.9 U.mL(-1).min(-1)) was recorded at 48 h which was 40.5% lower than the xylanase production by the immobilized cell (2.67 U.mL(-1).min(-1)) at the same time. The beads containing the immobilized cells could be reused up to eight fermentation cycles for xylanase production and retained 83.5% of the productivity at the fourth cycle. The entrapped cells were stable after six months of storage at 4 °C and retained 68% of the xylanase productivity. CONCLUSION: Cellulase free xylanase production from the immobilized Bacillus pumilus IMAU80221 was optimized. The xylanase production by the immobilized cells of Bacillus pumilus was higher by 40.5 and 132.6 % over the free cells respectively after 48 and 72 h of the incubation. |
format | Online Article Text |
id | pubmed-6697833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | National Institute of Genetic Engineering and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-66978332019-08-27 Optimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus Kundu, Aditi Majumdar, Bijan Iran J Biotechnol Research Article BACKGROUND: The extracellular xylanase secreted by microorganisms is a hydrolytic enzyme, which arbitrarily cleaves the β-1, 4 backbone of the polysaccharide xylan; an enzyme used in the food processing, bio-pulping and bio-bleaching. The commercial production of the xylanase is limited because of a higher cost involvement, which can be overcome by the cost-effective production of the xylanase through immobilization of the microbial cell by the non-toxic substances. OBJECTIVES: In this work, the optimization of the extra-cellular cellulase free xylanase production by the immobilized cell of the Bacillus pumilus IMAU80221 strain using Ca-alginate beads along with standardization of the various parameters for a higher xylanase production were studied. MATERIALS AND METHODS: Following to sterilization, the Na-alginate solution was mixed with the bacterial suspension of the Bacillus pumilus IMAU80221 and was added drop by drop into the 1 M calcium chloride solution for 1 h for obtaining a uniform sized polymeric bead of the Ca-alginate. For xylanase production, the Ca-alginate beads were then transferred into 100 mL Erlenmeyer flasks with 20 mL of the culture medium containing (w/v) 0.02% NaCl, 0.02% MgSO(4), 0.04% (KH(4))(2)PO(4), 0.1% peptone, and 0.5% xylan and incubated at 34 °C in an incubator shaker (150 rpm) for 24 h. The resultant supernatant (crude enzyme) was used for enzyme assay. RESULTS: The maximum xylanase production by the free cell (1.9 U.mL(-1).min(-1)) was recorded at 48 h which was 40.5% lower than the xylanase production by the immobilized cell (2.67 U.mL(-1).min(-1)) at the same time. The beads containing the immobilized cells could be reused up to eight fermentation cycles for xylanase production and retained 83.5% of the productivity at the fourth cycle. The entrapped cells were stable after six months of storage at 4 °C and retained 68% of the xylanase productivity. CONCLUSION: Cellulase free xylanase production from the immobilized Bacillus pumilus IMAU80221 was optimized. The xylanase production by the immobilized cells of Bacillus pumilus was higher by 40.5 and 132.6 % over the free cells respectively after 48 and 72 h of the incubation. National Institute of Genetic Engineering and Biotechnology 2018-12-12 /pmc/articles/PMC6697833/ /pubmed/31457031 http://dx.doi.org/10.21859/ijb.1658 Text en Copyright © 2018 The Author(s); Published by National Institute of Genetic Engineering and Biotechnology. http://creativecommons.org/licenses/by-nc/4.0/ This is an open access article, distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits others to copy and redistribute material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Research Article Kundu, Aditi Majumdar, Bijan Optimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus |
title | Optimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus |
title_full | Optimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus |
title_fullStr | Optimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus |
title_full_unstemmed | Optimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus |
title_short | Optimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus |
title_sort | optimization of the cellulase free xylanase production by immobilized bacillus pumilus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697833/ https://www.ncbi.nlm.nih.gov/pubmed/31457031 http://dx.doi.org/10.21859/ijb.1658 |
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