Cargando…

Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine

BACKGROUND: Newcastle disease is a major avian disease that causes enormous economic loss in poultry industry. There have been a number of reports on the suitability of plant-based recombinant vaccine against this disease. Fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle dis...

Descripción completa

Detalles Bibliográficos
Autores principales: Shahriari, Amir Ghaffar, Bagheri, Abdolreza, Afsharifar, Alireza, Habibi-Pirkoohi, Maziar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Genetic Engineering and Biotechnology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697852/
https://www.ncbi.nlm.nih.gov/pubmed/31457048
http://dx.doi.org/10.21859/ijb.2215
_version_ 1783444442886176768
author Shahriari, Amir Ghaffar
Bagheri, Abdolreza
Afsharifar, Alireza
Habibi-Pirkoohi, Maziar
author_facet Shahriari, Amir Ghaffar
Bagheri, Abdolreza
Afsharifar, Alireza
Habibi-Pirkoohi, Maziar
author_sort Shahriari, Amir Ghaffar
collection PubMed
description BACKGROUND: Newcastle disease is a major avian disease that causes enormous economic loss in poultry industry. There have been a number of reports on the suitability of plant-based recombinant vaccine against this disease. Fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle disease virus (NDV) represent the major immunogenic sites for development of recombinant anti-ND vaccines in plant hosts. OBJECTIVES: The main objective of this research was to evaluate the ability of a recombinant anti-ND vaccine in induction of immune responses in animal model. MATERIALS AND METHODS: In this study, immunogenicity of recombinant fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle disease virus (NDV) is investigated in an animal model. The corresponding genes encoding amino acids 65-81 of the F protein and 346-353 amino acids of HN were expressed in tobacco seedling using agrobacterium-mediated transformation. Expression of the foreign gene in the tobacco seedlings was investigated by a number of molecular assays including Real-Time PCR and ELISA. Transgenic plant extract was used to induce immunogenic response in animal model. RESULTS: Integration of the foreign gene in plant host genome was confirmed by polymerase chain reaction (PCR). Expression of the foreign recombinant protein was confirmed by Real-Time PCR and ELISA assays. Immunogenicity of the recombinant protein was investigated in rabbit by subcutaneous injection. Results indicated that the transgenic plant extract can induce immune responses in the host as confirmed by presence of specific antibodies in the sera in ELISA assay. Western blot assays showed that the foreign gene was actually expressed in transgenic seedlings. CONCLUSIONS: The results obtained in this research provide further evidence on applicability of plant-based recombinant vaccines for protection of poultry against Newcastle disease.
format Online
Article
Text
id pubmed-6697852
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher National Institute of Genetic Engineering and Biotechnology
record_format MEDLINE/PubMed
spelling pubmed-66978522019-08-27 Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine Shahriari, Amir Ghaffar Bagheri, Abdolreza Afsharifar, Alireza Habibi-Pirkoohi, Maziar Iran J Biotechnol Research Article BACKGROUND: Newcastle disease is a major avian disease that causes enormous economic loss in poultry industry. There have been a number of reports on the suitability of plant-based recombinant vaccine against this disease. Fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle disease virus (NDV) represent the major immunogenic sites for development of recombinant anti-ND vaccines in plant hosts. OBJECTIVES: The main objective of this research was to evaluate the ability of a recombinant anti-ND vaccine in induction of immune responses in animal model. MATERIALS AND METHODS: In this study, immunogenicity of recombinant fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle disease virus (NDV) is investigated in an animal model. The corresponding genes encoding amino acids 65-81 of the F protein and 346-353 amino acids of HN were expressed in tobacco seedling using agrobacterium-mediated transformation. Expression of the foreign gene in the tobacco seedlings was investigated by a number of molecular assays including Real-Time PCR and ELISA. Transgenic plant extract was used to induce immunogenic response in animal model. RESULTS: Integration of the foreign gene in plant host genome was confirmed by polymerase chain reaction (PCR). Expression of the foreign recombinant protein was confirmed by Real-Time PCR and ELISA assays. Immunogenicity of the recombinant protein was investigated in rabbit by subcutaneous injection. Results indicated that the transgenic plant extract can induce immune responses in the host as confirmed by presence of specific antibodies in the sera in ELISA assay. Western blot assays showed that the foreign gene was actually expressed in transgenic seedlings. CONCLUSIONS: The results obtained in this research provide further evidence on applicability of plant-based recombinant vaccines for protection of poultry against Newcastle disease. National Institute of Genetic Engineering and Biotechnology 2019-01-11 /pmc/articles/PMC6697852/ /pubmed/31457048 http://dx.doi.org/10.21859/ijb.2215 Text en Copyright © 2019 The Author(s); Published by National Institute of Genetic Engineering and Biotechnology. http://creativecommons.org/licenses/by-nc/4.0/ This is an open access article, distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits others to copy and redistribute material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Shahriari, Amir Ghaffar
Bagheri, Abdolreza
Afsharifar, Alireza
Habibi-Pirkoohi, Maziar
Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine
title Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine
title_full Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine
title_fullStr Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine
title_full_unstemmed Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine
title_short Induction of Immune Response in Animal Model Using Recombinant Anti-NDV Vaccine
title_sort induction of immune response in animal model using recombinant anti-ndv vaccine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6697852/
https://www.ncbi.nlm.nih.gov/pubmed/31457048
http://dx.doi.org/10.21859/ijb.2215
work_keys_str_mv AT shahriariamirghaffar inductionofimmuneresponseinanimalmodelusingrecombinantantindvvaccine
AT bagheriabdolreza inductionofimmuneresponseinanimalmodelusingrecombinantantindvvaccine
AT afsharifaralireza inductionofimmuneresponseinanimalmodelusingrecombinantantindvvaccine
AT habibipirkoohimaziar inductionofimmuneresponseinanimalmodelusingrecombinantantindvvaccine