Poly-β-hydroxybutyrate Production by Methylosinus trichosporium OB3b at Different Gas-phase Conditions

BACKGROUND: The utilization of methane for production of Poly-β-hydroxybutyrate (PHB) not only cuts the emissions of greenhouse gases but also greatly reduces PHB production cost. OBJECTIVES: The aim of this study was to determine the effects of gas-phase conditions on PHB production by Methylosinus trichosporium OB(3)b. MATERIALS AND METHODS: Bacterial cultivation and PHB production were conducted in a series of sealed serum bottles. Nitrogen-free mineral salts medium was used to induce PHB production in the presence or absence of N(2) in the headspace. RESULTS: In the absence of N(2), the highest PHB content (i.e., 52.9% of the dry cell weight with a PHB concentration of 814.3 mg.L(-1)) was obtained at a ratio of CH(4):O(2)=2:1. Further study at different O(2) concentrations with a fixed CH(4) partial pressure in absence of N(2) showed that PHB accumulation by methanotroph could be tolerated high oxygen partial pressure and its respond to the variation of the oxygen concentration depends on the methane partial pressure. In presence of N(2), with headspace gas replenished only when oxygen was almost depleted, the degradation of intracellular PHB has appeared. In the regimen of updating headspace gas at the point when the PHB content began to decrease, the highest PHB content (i.e., 55.5% of the dry cell weight with 901.8 mg.L(-1) PHB concentration and 12.5 mg.L(-1).h(-1)PHB productivity) was obtained at 0.2 atm O(2) and PHB accumulation was depressed with an oxygen concentration greater than 0.3 atm. CONCLUSIONS: The methanotroph responses differentially to the increase in the oxygen partial pressure with regard to PHB accumulation either in the presence or in the absence of N2.