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Prognostic value of microvessel density in stage II and III colon cancer patients: a retrospective cohort study
BACKGROUND: Microvessel density (MVD), as a derived marker for angiogenesis, has been associated with poor outcome in several types of cancer. This study aimed to evaluate the prognostic value of MVD in stage II and III colon cancer and its relation to tumour-stroma-percentage (TSP) and expression o...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698008/ https://www.ncbi.nlm.nih.gov/pubmed/31420015 http://dx.doi.org/10.1186/s12876-019-1063-4 |
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author | den Uil, Sjoerd H. van den Broek, Evert Coupé, Veerle M. H. Vellinga, Thomas T. Delis-van Diemen, Pien M. Bril, Herman Belt, Eric J. Th. Kranenburg, Onno Stockmann, Hein B. A. C. Belien, Jeroen A. M. Meijer, Gerrit A. Fijneman, Remond J. A. |
author_facet | den Uil, Sjoerd H. van den Broek, Evert Coupé, Veerle M. H. Vellinga, Thomas T. Delis-van Diemen, Pien M. Bril, Herman Belt, Eric J. Th. Kranenburg, Onno Stockmann, Hein B. A. C. Belien, Jeroen A. M. Meijer, Gerrit A. Fijneman, Remond J. A. |
author_sort | den Uil, Sjoerd H. |
collection | PubMed |
description | BACKGROUND: Microvessel density (MVD), as a derived marker for angiogenesis, has been associated with poor outcome in several types of cancer. This study aimed to evaluate the prognostic value of MVD in stage II and III colon cancer and its relation to tumour-stroma-percentage (TSP) and expression of HIF1A and VEGFA. METHODS: Formalin-fixed paraffin-embedded (FFPE) colon cancer tissues were collected from 53 stage II and 54 (5-fluorouracil-treated) stage III patients. MVD was scored by digital morphometric analysis of CD31-stained whole tumour sections. TSP was scored using haematoxylin-eosin stained slides. Protein expression of HIF1A and VEGFA was determined by immunohistochemical evaluation of tissue microarrays. RESULTS: Median MVD was higher in stage III compared to stage II colon cancers (11.1% versus 5.6% CD31-positive tissue area, p < 0.001). High MVD in stage II patients tended to be associated with poor disease free survival (DFS) in univariate analysis (p = 0.056). In contrast, high MVD in 5FU-treated stage III patients was associated with better DFS (p = 0.006). Prognostic value for MVD was observed in multivariate analyses for both cancer stages. CONCLUSIONS: MVD is an independent prognostic factor associated with poor DFS in stage II colon cancer patients, and with better DFS in stage III colon cancer patients treated with adjuvant chemotherapy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12876-019-1063-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6698008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-66980082019-08-19 Prognostic value of microvessel density in stage II and III colon cancer patients: a retrospective cohort study den Uil, Sjoerd H. van den Broek, Evert Coupé, Veerle M. H. Vellinga, Thomas T. Delis-van Diemen, Pien M. Bril, Herman Belt, Eric J. Th. Kranenburg, Onno Stockmann, Hein B. A. C. Belien, Jeroen A. M. Meijer, Gerrit A. Fijneman, Remond J. A. BMC Gastroenterol Research Article BACKGROUND: Microvessel density (MVD), as a derived marker for angiogenesis, has been associated with poor outcome in several types of cancer. This study aimed to evaluate the prognostic value of MVD in stage II and III colon cancer and its relation to tumour-stroma-percentage (TSP) and expression of HIF1A and VEGFA. METHODS: Formalin-fixed paraffin-embedded (FFPE) colon cancer tissues were collected from 53 stage II and 54 (5-fluorouracil-treated) stage III patients. MVD was scored by digital morphometric analysis of CD31-stained whole tumour sections. TSP was scored using haematoxylin-eosin stained slides. Protein expression of HIF1A and VEGFA was determined by immunohistochemical evaluation of tissue microarrays. RESULTS: Median MVD was higher in stage III compared to stage II colon cancers (11.1% versus 5.6% CD31-positive tissue area, p < 0.001). High MVD in stage II patients tended to be associated with poor disease free survival (DFS) in univariate analysis (p = 0.056). In contrast, high MVD in 5FU-treated stage III patients was associated with better DFS (p = 0.006). Prognostic value for MVD was observed in multivariate analyses for both cancer stages. CONCLUSIONS: MVD is an independent prognostic factor associated with poor DFS in stage II colon cancer patients, and with better DFS in stage III colon cancer patients treated with adjuvant chemotherapy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12876-019-1063-4) contains supplementary material, which is available to authorized users. BioMed Central 2019-08-16 /pmc/articles/PMC6698008/ /pubmed/31420015 http://dx.doi.org/10.1186/s12876-019-1063-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article den Uil, Sjoerd H. van den Broek, Evert Coupé, Veerle M. H. Vellinga, Thomas T. Delis-van Diemen, Pien M. Bril, Herman Belt, Eric J. Th. Kranenburg, Onno Stockmann, Hein B. A. C. Belien, Jeroen A. M. Meijer, Gerrit A. Fijneman, Remond J. A. Prognostic value of microvessel density in stage II and III colon cancer patients: a retrospective cohort study |
title | Prognostic value of microvessel density in stage II and III colon cancer patients: a retrospective cohort study |
title_full | Prognostic value of microvessel density in stage II and III colon cancer patients: a retrospective cohort study |
title_fullStr | Prognostic value of microvessel density in stage II and III colon cancer patients: a retrospective cohort study |
title_full_unstemmed | Prognostic value of microvessel density in stage II and III colon cancer patients: a retrospective cohort study |
title_short | Prognostic value of microvessel density in stage II and III colon cancer patients: a retrospective cohort study |
title_sort | prognostic value of microvessel density in stage ii and iii colon cancer patients: a retrospective cohort study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698008/ https://www.ncbi.nlm.nih.gov/pubmed/31420015 http://dx.doi.org/10.1186/s12876-019-1063-4 |
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