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Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice

PURPOSE: Multiwalled carbon nanotubes (MWCNTs) have been known to enter the circulatory system via the lungs from inhalation exposure; however, its carcinogenicity and subsequent accumulation in other organs have not been adequately reported in the literature. Moreover, the safety of MWCNTs as a bio...

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Autores principales: Sobajima, Atsushi, Haniu, Hisao, Nomura, Hiroki, Tanaka, Manabu, Takizawa, Takashi, Kamanaka, Takayuki, Aoki, Kaoru, Okamoto, Masanori, Yoshida, Kazushige, Sasaki, Jun, Ajima, Kumiko, Kuroda, Chika, Ishida, Haruka, Okano, Satomi, Ueda, Katsuya, Kato, Hiroyuki, Saito, Naoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698589/
https://www.ncbi.nlm.nih.gov/pubmed/31616140
http://dx.doi.org/10.2147/IJN.S208129
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author Sobajima, Atsushi
Haniu, Hisao
Nomura, Hiroki
Tanaka, Manabu
Takizawa, Takashi
Kamanaka, Takayuki
Aoki, Kaoru
Okamoto, Masanori
Yoshida, Kazushige
Sasaki, Jun
Ajima, Kumiko
Kuroda, Chika
Ishida, Haruka
Okano, Satomi
Ueda, Katsuya
Kato, Hiroyuki
Saito, Naoto
author_facet Sobajima, Atsushi
Haniu, Hisao
Nomura, Hiroki
Tanaka, Manabu
Takizawa, Takashi
Kamanaka, Takayuki
Aoki, Kaoru
Okamoto, Masanori
Yoshida, Kazushige
Sasaki, Jun
Ajima, Kumiko
Kuroda, Chika
Ishida, Haruka
Okano, Satomi
Ueda, Katsuya
Kato, Hiroyuki
Saito, Naoto
author_sort Sobajima, Atsushi
collection PubMed
description PURPOSE: Multiwalled carbon nanotubes (MWCNTs) have been known to enter the circulatory system via the lungs from inhalation exposure; however, its carcinogenicity and subsequent accumulation in other organs have not been adequately reported in the literature. Moreover, the safety of MWCNTs as a biomaterial has remained a matter of debate, particularly when the material enters the circulatory system. To address these problems, we used carcinogenic rasH2 transgenic mice to intravenously administer highly dispersed MWCNTs and to evaluate their carcinogenicity and accumulation in the organs. METHODS: Two types of MWCNTs (thin- and thick-MWCNTs) were intravenously administered at a high dose (approximately 0.7 mg per kg body weight) and low dose (approximately 0.07 mg per kg body weight). RESULTS: MWCNTs showed pancreatic accumulation in 3.2% of mice administered with MWCNTs, but there was no accumulation in other organs. In addition, there was no significant difference in the incidence of tumor among the four MWCNTs-administered groups compared to the vehicle group without MWCNTs administration. Blood tests revealed elevated levels in mean red blood cell volume and mean red blood cell hemoglobin level for the MWCNTs-administered group, in addition to an increase in eotaxin. CONCLUSION: The present study demonstrated that the use of current technology to sufficiently disperse MWCNTs resulted in minimal organ accumulation with no evidence of carcinogenicity.
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spelling pubmed-66985892019-10-15 Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice Sobajima, Atsushi Haniu, Hisao Nomura, Hiroki Tanaka, Manabu Takizawa, Takashi Kamanaka, Takayuki Aoki, Kaoru Okamoto, Masanori Yoshida, Kazushige Sasaki, Jun Ajima, Kumiko Kuroda, Chika Ishida, Haruka Okano, Satomi Ueda, Katsuya Kato, Hiroyuki Saito, Naoto Int J Nanomedicine Original Research PURPOSE: Multiwalled carbon nanotubes (MWCNTs) have been known to enter the circulatory system via the lungs from inhalation exposure; however, its carcinogenicity and subsequent accumulation in other organs have not been adequately reported in the literature. Moreover, the safety of MWCNTs as a biomaterial has remained a matter of debate, particularly when the material enters the circulatory system. To address these problems, we used carcinogenic rasH2 transgenic mice to intravenously administer highly dispersed MWCNTs and to evaluate their carcinogenicity and accumulation in the organs. METHODS: Two types of MWCNTs (thin- and thick-MWCNTs) were intravenously administered at a high dose (approximately 0.7 mg per kg body weight) and low dose (approximately 0.07 mg per kg body weight). RESULTS: MWCNTs showed pancreatic accumulation in 3.2% of mice administered with MWCNTs, but there was no accumulation in other organs. In addition, there was no significant difference in the incidence of tumor among the four MWCNTs-administered groups compared to the vehicle group without MWCNTs administration. Blood tests revealed elevated levels in mean red blood cell volume and mean red blood cell hemoglobin level for the MWCNTs-administered group, in addition to an increase in eotaxin. CONCLUSION: The present study demonstrated that the use of current technology to sufficiently disperse MWCNTs resulted in minimal organ accumulation with no evidence of carcinogenicity. Dove 2019-08-12 /pmc/articles/PMC6698589/ /pubmed/31616140 http://dx.doi.org/10.2147/IJN.S208129 Text en © 2019 Sobajima et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Sobajima, Atsushi
Haniu, Hisao
Nomura, Hiroki
Tanaka, Manabu
Takizawa, Takashi
Kamanaka, Takayuki
Aoki, Kaoru
Okamoto, Masanori
Yoshida, Kazushige
Sasaki, Jun
Ajima, Kumiko
Kuroda, Chika
Ishida, Haruka
Okano, Satomi
Ueda, Katsuya
Kato, Hiroyuki
Saito, Naoto
Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice
title Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice
title_full Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice
title_fullStr Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice
title_full_unstemmed Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice
title_short Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice
title_sort organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rash2 mice
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698589/
https://www.ncbi.nlm.nih.gov/pubmed/31616140
http://dx.doi.org/10.2147/IJN.S208129
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