Cargando…
Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice
PURPOSE: Multiwalled carbon nanotubes (MWCNTs) have been known to enter the circulatory system via the lungs from inhalation exposure; however, its carcinogenicity and subsequent accumulation in other organs have not been adequately reported in the literature. Moreover, the safety of MWCNTs as a bio...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698589/ https://www.ncbi.nlm.nih.gov/pubmed/31616140 http://dx.doi.org/10.2147/IJN.S208129 |
_version_ | 1783444574294769664 |
---|---|
author | Sobajima, Atsushi Haniu, Hisao Nomura, Hiroki Tanaka, Manabu Takizawa, Takashi Kamanaka, Takayuki Aoki, Kaoru Okamoto, Masanori Yoshida, Kazushige Sasaki, Jun Ajima, Kumiko Kuroda, Chika Ishida, Haruka Okano, Satomi Ueda, Katsuya Kato, Hiroyuki Saito, Naoto |
author_facet | Sobajima, Atsushi Haniu, Hisao Nomura, Hiroki Tanaka, Manabu Takizawa, Takashi Kamanaka, Takayuki Aoki, Kaoru Okamoto, Masanori Yoshida, Kazushige Sasaki, Jun Ajima, Kumiko Kuroda, Chika Ishida, Haruka Okano, Satomi Ueda, Katsuya Kato, Hiroyuki Saito, Naoto |
author_sort | Sobajima, Atsushi |
collection | PubMed |
description | PURPOSE: Multiwalled carbon nanotubes (MWCNTs) have been known to enter the circulatory system via the lungs from inhalation exposure; however, its carcinogenicity and subsequent accumulation in other organs have not been adequately reported in the literature. Moreover, the safety of MWCNTs as a biomaterial has remained a matter of debate, particularly when the material enters the circulatory system. To address these problems, we used carcinogenic rasH2 transgenic mice to intravenously administer highly dispersed MWCNTs and to evaluate their carcinogenicity and accumulation in the organs. METHODS: Two types of MWCNTs (thin- and thick-MWCNTs) were intravenously administered at a high dose (approximately 0.7 mg per kg body weight) and low dose (approximately 0.07 mg per kg body weight). RESULTS: MWCNTs showed pancreatic accumulation in 3.2% of mice administered with MWCNTs, but there was no accumulation in other organs. In addition, there was no significant difference in the incidence of tumor among the four MWCNTs-administered groups compared to the vehicle group without MWCNTs administration. Blood tests revealed elevated levels in mean red blood cell volume and mean red blood cell hemoglobin level for the MWCNTs-administered group, in addition to an increase in eotaxin. CONCLUSION: The present study demonstrated that the use of current technology to sufficiently disperse MWCNTs resulted in minimal organ accumulation with no evidence of carcinogenicity. |
format | Online Article Text |
id | pubmed-6698589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-66985892019-10-15 Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice Sobajima, Atsushi Haniu, Hisao Nomura, Hiroki Tanaka, Manabu Takizawa, Takashi Kamanaka, Takayuki Aoki, Kaoru Okamoto, Masanori Yoshida, Kazushige Sasaki, Jun Ajima, Kumiko Kuroda, Chika Ishida, Haruka Okano, Satomi Ueda, Katsuya Kato, Hiroyuki Saito, Naoto Int J Nanomedicine Original Research PURPOSE: Multiwalled carbon nanotubes (MWCNTs) have been known to enter the circulatory system via the lungs from inhalation exposure; however, its carcinogenicity and subsequent accumulation in other organs have not been adequately reported in the literature. Moreover, the safety of MWCNTs as a biomaterial has remained a matter of debate, particularly when the material enters the circulatory system. To address these problems, we used carcinogenic rasH2 transgenic mice to intravenously administer highly dispersed MWCNTs and to evaluate their carcinogenicity and accumulation in the organs. METHODS: Two types of MWCNTs (thin- and thick-MWCNTs) were intravenously administered at a high dose (approximately 0.7 mg per kg body weight) and low dose (approximately 0.07 mg per kg body weight). RESULTS: MWCNTs showed pancreatic accumulation in 3.2% of mice administered with MWCNTs, but there was no accumulation in other organs. In addition, there was no significant difference in the incidence of tumor among the four MWCNTs-administered groups compared to the vehicle group without MWCNTs administration. Blood tests revealed elevated levels in mean red blood cell volume and mean red blood cell hemoglobin level for the MWCNTs-administered group, in addition to an increase in eotaxin. CONCLUSION: The present study demonstrated that the use of current technology to sufficiently disperse MWCNTs resulted in minimal organ accumulation with no evidence of carcinogenicity. Dove 2019-08-12 /pmc/articles/PMC6698589/ /pubmed/31616140 http://dx.doi.org/10.2147/IJN.S208129 Text en © 2019 Sobajima et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Sobajima, Atsushi Haniu, Hisao Nomura, Hiroki Tanaka, Manabu Takizawa, Takashi Kamanaka, Takayuki Aoki, Kaoru Okamoto, Masanori Yoshida, Kazushige Sasaki, Jun Ajima, Kumiko Kuroda, Chika Ishida, Haruka Okano, Satomi Ueda, Katsuya Kato, Hiroyuki Saito, Naoto Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice |
title | Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice |
title_full | Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice |
title_fullStr | Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice |
title_full_unstemmed | Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice |
title_short | Organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rasH2 mice |
title_sort | organ accumulation and carcinogenicity of highly dispersed multi-walled carbon nanotubes administered intravenously in transgenic rash2 mice |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698589/ https://www.ncbi.nlm.nih.gov/pubmed/31616140 http://dx.doi.org/10.2147/IJN.S208129 |
work_keys_str_mv | AT sobajimaatsushi organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT haniuhisao organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT nomurahiroki organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT tanakamanabu organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT takizawatakashi organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT kamanakatakayuki organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT aokikaoru organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT okamotomasanori organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT yoshidakazushige organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT sasakijun organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT ajimakumiko organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT kurodachika organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT ishidaharuka organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT okanosatomi organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT uedakatsuya organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT katohiroyuki organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice AT saitonaoto organaccumulationandcarcinogenicityofhighlydispersedmultiwalledcarbonnanotubesadministeredintravenouslyintransgenicrash2mice |