Microhomologies are prevalent at Cas9-induced larger deletions

The CRISPR system is widely used in genome editing for biomedical research. Here, using either dual paired Cas9(D10A) nickases or paired Cas9 nuclease we characterize unintended larger deletions at on-target sites that frequently evade common genotyping practices. We found that unintended larger del...

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Detalles Bibliográficos
Autores principales: Owens, Dominic D G, Caulder, Adam, Frontera, Vincent, Harman, Joe R, Allan, Alasdair J, Bucakci, Akin, Greder, Lucas, Codner, Gemma F, Hublitz, Philip, McHugh, Peter J, Teboul, Lydia, de Bruijn, Marella F T R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698657/
https://www.ncbi.nlm.nih.gov/pubmed/31127293
http://dx.doi.org/10.1093/nar/gkz459

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