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CRISPR repeat sequences and relative spacing specify DNA integration by Pyrococcus furiosus Cas1 and Cas2

Acquiring foreign spacer DNA into the CRISPR locus is an essential primary step of the CRISPR–Cas pathway in prokaryotes for developing host immunity to mobile genetic elements. Here, we investigate spacer integration in vitro using proteins from Pyrococcus furiosus and demonstrate that Cas1 and Cas...

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Detalles Bibliográficos
Autores principales:	Grainy, Julie, Garrett, Sandra, Graveley, Brenton R, P. Terns, Michael
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2019
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698737/ https://www.ncbi.nlm.nih.gov/pubmed/31219587 http://dx.doi.org/10.1093/nar/gkz548

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