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Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies
Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn(+)) cells are a po...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698821/ https://www.ncbi.nlm.nih.gov/pubmed/31557988 http://dx.doi.org/10.3390/antib5020007 |
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author | De Schryver, Marjorie Van Gorp, Hanne Hoebeke, Inge De Maeyer, Bauke Ooms, Karen Pintelon, Isabel Maes, Louis J. Cos, Paul Nauwynck, Hans J. Delputte, Peter L. |
author_facet | De Schryver, Marjorie Van Gorp, Hanne Hoebeke, Inge De Maeyer, Bauke Ooms, Karen Pintelon, Isabel Maes, Louis J. Cos, Paul Nauwynck, Hans J. Delputte, Peter L. |
author_sort | De Schryver, Marjorie |
collection | PubMed |
description | Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn(+)) cells are a potential strategy for targeted treatment. It has been shown that Sn internalizes after binding with a mAb, though it is not clear whether this is species-specific. In this study, new Sn-specific mAbs were developed and analyzed for cross-reactivity between species. In addition, the newly developed mAbs were compared to mAbs used in previous research for their epitope recognition and other Sn-specific characteristics. Both species-specific and cross-reactive antibodies could be identified. Furthermore, sialic acid-binding of red blood cells (RBC) could be inhibited with mAbs recognizing different epitopes and all mAb showed internalization of Sn. The newly developed mAbs can be used as novel tools for Sn research and further analysis of Sn internalization in different species. |
format | Online Article Text |
id | pubmed-6698821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66988212019-09-05 Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies De Schryver, Marjorie Van Gorp, Hanne Hoebeke, Inge De Maeyer, Bauke Ooms, Karen Pintelon, Isabel Maes, Louis J. Cos, Paul Nauwynck, Hans J. Delputte, Peter L. Antibodies (Basel) Article Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn(+)) cells are a potential strategy for targeted treatment. It has been shown that Sn internalizes after binding with a mAb, though it is not clear whether this is species-specific. In this study, new Sn-specific mAbs were developed and analyzed for cross-reactivity between species. In addition, the newly developed mAbs were compared to mAbs used in previous research for their epitope recognition and other Sn-specific characteristics. Both species-specific and cross-reactive antibodies could be identified. Furthermore, sialic acid-binding of red blood cells (RBC) could be inhibited with mAbs recognizing different epitopes and all mAb showed internalization of Sn. The newly developed mAbs can be used as novel tools for Sn research and further analysis of Sn internalization in different species. MDPI 2016-03-23 /pmc/articles/PMC6698821/ /pubmed/31557988 http://dx.doi.org/10.3390/antib5020007 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article De Schryver, Marjorie Van Gorp, Hanne Hoebeke, Inge De Maeyer, Bauke Ooms, Karen Pintelon, Isabel Maes, Louis J. Cos, Paul Nauwynck, Hans J. Delputte, Peter L. Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies |
title | Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies |
title_full | Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies |
title_fullStr | Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies |
title_full_unstemmed | Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies |
title_short | Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies |
title_sort | development and characterization of new species cross-reactive anti-sialoadhesin monoclonal antibodies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698821/ https://www.ncbi.nlm.nih.gov/pubmed/31557988 http://dx.doi.org/10.3390/antib5020007 |
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