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Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies

Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn(+)) cells are a po...

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Autores principales: De Schryver, Marjorie, Van Gorp, Hanne, Hoebeke, Inge, De Maeyer, Bauke, Ooms, Karen, Pintelon, Isabel, Maes, Louis J., Cos, Paul, Nauwynck, Hans J., Delputte, Peter L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698821/
https://www.ncbi.nlm.nih.gov/pubmed/31557988
http://dx.doi.org/10.3390/antib5020007
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author De Schryver, Marjorie
Van Gorp, Hanne
Hoebeke, Inge
De Maeyer, Bauke
Ooms, Karen
Pintelon, Isabel
Maes, Louis J.
Cos, Paul
Nauwynck, Hans J.
Delputte, Peter L.
author_facet De Schryver, Marjorie
Van Gorp, Hanne
Hoebeke, Inge
De Maeyer, Bauke
Ooms, Karen
Pintelon, Isabel
Maes, Louis J.
Cos, Paul
Nauwynck, Hans J.
Delputte, Peter L.
author_sort De Schryver, Marjorie
collection PubMed
description Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn(+)) cells are a potential strategy for targeted treatment. It has been shown that Sn internalizes after binding with a mAb, though it is not clear whether this is species-specific. In this study, new Sn-specific mAbs were developed and analyzed for cross-reactivity between species. In addition, the newly developed mAbs were compared to mAbs used in previous research for their epitope recognition and other Sn-specific characteristics. Both species-specific and cross-reactive antibodies could be identified. Furthermore, sialic acid-binding of red blood cells (RBC) could be inhibited with mAbs recognizing different epitopes and all mAb showed internalization of Sn. The newly developed mAbs can be used as novel tools for Sn research and further analysis of Sn internalization in different species.
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spelling pubmed-66988212019-09-05 Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies De Schryver, Marjorie Van Gorp, Hanne Hoebeke, Inge De Maeyer, Bauke Ooms, Karen Pintelon, Isabel Maes, Louis J. Cos, Paul Nauwynck, Hans J. Delputte, Peter L. Antibodies (Basel) Article Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn(+)) cells are a potential strategy for targeted treatment. It has been shown that Sn internalizes after binding with a mAb, though it is not clear whether this is species-specific. In this study, new Sn-specific mAbs were developed and analyzed for cross-reactivity between species. In addition, the newly developed mAbs were compared to mAbs used in previous research for their epitope recognition and other Sn-specific characteristics. Both species-specific and cross-reactive antibodies could be identified. Furthermore, sialic acid-binding of red blood cells (RBC) could be inhibited with mAbs recognizing different epitopes and all mAb showed internalization of Sn. The newly developed mAbs can be used as novel tools for Sn research and further analysis of Sn internalization in different species. MDPI 2016-03-23 /pmc/articles/PMC6698821/ /pubmed/31557988 http://dx.doi.org/10.3390/antib5020007 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Schryver, Marjorie
Van Gorp, Hanne
Hoebeke, Inge
De Maeyer, Bauke
Ooms, Karen
Pintelon, Isabel
Maes, Louis J.
Cos, Paul
Nauwynck, Hans J.
Delputte, Peter L.
Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies
title Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies
title_full Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies
title_fullStr Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies
title_full_unstemmed Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies
title_short Development and Characterization of New Species Cross-Reactive Anti-Sialoadhesin Monoclonal Antibodies
title_sort development and characterization of new species cross-reactive anti-sialoadhesin monoclonal antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698821/
https://www.ncbi.nlm.nih.gov/pubmed/31557988
http://dx.doi.org/10.3390/antib5020007
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