Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody

Isocitrate dehydrogenase 1 (IDH1) gene mutations have been observed in a majority of diffuse astrocytomas, oligodendrogliomas, and secondary glioblastomas, and the mutant IDH1 R132H is detectable in most of these lesions. By specifically targeting the R132H mutation through B-cell cloning, a novel r...

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Autores principales: Rashidian, Juliet, Copaciu, Raul, Su, Qin, Merritt, Brett, Johnson, Claire, Yahyabeik, Aril, French, Ella, Cummings, Kelsea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698843/
https://www.ncbi.nlm.nih.gov/pubmed/31548536
http://dx.doi.org/10.3390/antib6040022
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author Rashidian, Juliet
Copaciu, Raul
Su, Qin
Merritt, Brett
Johnson, Claire
Yahyabeik, Aril
French, Ella
Cummings, Kelsea
author_facet Rashidian, Juliet
Copaciu, Raul
Su, Qin
Merritt, Brett
Johnson, Claire
Yahyabeik, Aril
French, Ella
Cummings, Kelsea
author_sort Rashidian, Juliet
collection PubMed
description Isocitrate dehydrogenase 1 (IDH1) gene mutations have been observed in a majority of diffuse astrocytomas, oligodendrogliomas, and secondary glioblastomas, and the mutant IDH1 R132H is detectable in most of these lesions. By specifically targeting the R132H mutation through B-cell cloning, a novel rabbit monoclonal antibody, MRQ-67, was produced that can recognize mutant IDH1 R132H and does not react with the wild type protein as demonstrated by Enzyme-linked immunosorbent assay (ELISA) and Western blotting. Through immunohistochemistry, the antibody is able to highlight neoplastic cells in glioma tissue specimens, and can be used as a tool in glioma subtyping. Immunohistochemistry (IHC) detection of IDH1 mutant protein may also be used to visualize single infiltrating tumor cells in surrounding brain tissue with an otherwise normal appearance.
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spelling pubmed-66988432019-09-05 Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody Rashidian, Juliet Copaciu, Raul Su, Qin Merritt, Brett Johnson, Claire Yahyabeik, Aril French, Ella Cummings, Kelsea Antibodies (Basel) Article Isocitrate dehydrogenase 1 (IDH1) gene mutations have been observed in a majority of diffuse astrocytomas, oligodendrogliomas, and secondary glioblastomas, and the mutant IDH1 R132H is detectable in most of these lesions. By specifically targeting the R132H mutation through B-cell cloning, a novel rabbit monoclonal antibody, MRQ-67, was produced that can recognize mutant IDH1 R132H and does not react with the wild type protein as demonstrated by Enzyme-linked immunosorbent assay (ELISA) and Western blotting. Through immunohistochemistry, the antibody is able to highlight neoplastic cells in glioma tissue specimens, and can be used as a tool in glioma subtyping. Immunohistochemistry (IHC) detection of IDH1 mutant protein may also be used to visualize single infiltrating tumor cells in surrounding brain tissue with an otherwise normal appearance. MDPI 2017-12-01 /pmc/articles/PMC6698843/ /pubmed/31548536 http://dx.doi.org/10.3390/antib6040022 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rashidian, Juliet
Copaciu, Raul
Su, Qin
Merritt, Brett
Johnson, Claire
Yahyabeik, Aril
French, Ella
Cummings, Kelsea
Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody
title Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody
title_full Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody
title_fullStr Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody
title_full_unstemmed Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody
title_short Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody
title_sort generation and performance of r132h mutant idh1 rabbit monoclonal antibody
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6698843/
https://www.ncbi.nlm.nih.gov/pubmed/31548536
http://dx.doi.org/10.3390/antib6040022
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