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Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam

Background. Clonorchis sinensis/Opisthorchis viverrini and minute intestinal flukes (MIF) such as Haplorchis pumilio and H. taichui are fish-borne trematodes (FBT) that may coexist in regions where local people have a habit of eating raw fish like Vietnam. Responses to FBT should be verified accordi...

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Autores principales: Tran, Anh Kieu Thi, Doan, Hoa Thuy, Do, Anh Ngoc, Nguyen, Van Thi, Hoang, Su Xuan, Le, Huong Thu Thi, Hoang, Hoa Thi, Le, Nam Hoang, Le, Quyen Bao Thi, Le, Tran-Anh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6699318/
https://www.ncbi.nlm.nih.gov/pubmed/31467915
http://dx.doi.org/10.1155/2019/8581379
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author Tran, Anh Kieu Thi
Doan, Hoa Thuy
Do, Anh Ngoc
Nguyen, Van Thi
Hoang, Su Xuan
Le, Huong Thu Thi
Hoang, Hoa Thi
Le, Nam Hoang
Le, Quyen Bao Thi
Le, Tran-Anh
author_facet Tran, Anh Kieu Thi
Doan, Hoa Thuy
Do, Anh Ngoc
Nguyen, Van Thi
Hoang, Su Xuan
Le, Huong Thu Thi
Hoang, Hoa Thi
Le, Nam Hoang
Le, Quyen Bao Thi
Le, Tran-Anh
author_sort Tran, Anh Kieu Thi
collection PubMed
description Background. Clonorchis sinensis/Opisthorchis viverrini and minute intestinal flukes (MIF) such as Haplorchis pumilio and H. taichui are fish-borne trematodes (FBT) that may coexist in regions where local people have a habit of eating raw fish like Vietnam. Responses to FBT should be verified according to the data on the distribution of these flukes. This study aims to explore the prevalence of different species of FBT and related factors among local people in a northern province of Vietnam. Methods. A cross-sectional study was conducted in Kim Son and Yen Khanh districts, Ninh Binh province, between March 2016 and March 2017. Four hundred people aged 15 years or older were interviewed and gave stool samples. The FBT eggs in faecal samples were enumerated by modified formalin-ether technique and identified by sequencing of the second internal transcribed spacer (ITS2) region. Result. Among the 400 persons, 19.5% were infected with FBT. On univariate analysis, eating raw fish was the main risk factor (odds ratios (OR)) of 6.769 (95% confidence interval (CI) of 2.655–17.259) followed by being of male gender (3.994 (CI95% 2.117–7.536)) and drinking alcohol (2.680 (CI95% 1.440–4.986)), respectively. There was no risk of increased infection among those living at home without hygienic latrines, those living close to rivers or having ponds, or those raising cats or dogs. By multivariate analysis, FBT infection was only related to the consumption of raw fish and gender. Seventy stool samples with a sufficient amount of faecal matter were subjected to DNA extraction, 42.85% of them yielded DNA production, and all were of Clonorchis sinensis. Conclusion. Results of the study showed the high prevalence of infection of fish-borne trematode, mostly C. sinensis among humans in Ninh Binh province. The prevention of FBT should be strengthened with programs detailed according to the distribution of FBT in different endemic areas.
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spelling pubmed-66993182019-08-29 Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam Tran, Anh Kieu Thi Doan, Hoa Thuy Do, Anh Ngoc Nguyen, Van Thi Hoang, Su Xuan Le, Huong Thu Thi Hoang, Hoa Thi Le, Nam Hoang Le, Quyen Bao Thi Le, Tran-Anh Biomed Res Int Research Article Background. Clonorchis sinensis/Opisthorchis viverrini and minute intestinal flukes (MIF) such as Haplorchis pumilio and H. taichui are fish-borne trematodes (FBT) that may coexist in regions where local people have a habit of eating raw fish like Vietnam. Responses to FBT should be verified according to the data on the distribution of these flukes. This study aims to explore the prevalence of different species of FBT and related factors among local people in a northern province of Vietnam. Methods. A cross-sectional study was conducted in Kim Son and Yen Khanh districts, Ninh Binh province, between March 2016 and March 2017. Four hundred people aged 15 years or older were interviewed and gave stool samples. The FBT eggs in faecal samples were enumerated by modified formalin-ether technique and identified by sequencing of the second internal transcribed spacer (ITS2) region. Result. Among the 400 persons, 19.5% were infected with FBT. On univariate analysis, eating raw fish was the main risk factor (odds ratios (OR)) of 6.769 (95% confidence interval (CI) of 2.655–17.259) followed by being of male gender (3.994 (CI95% 2.117–7.536)) and drinking alcohol (2.680 (CI95% 1.440–4.986)), respectively. There was no risk of increased infection among those living at home without hygienic latrines, those living close to rivers or having ponds, or those raising cats or dogs. By multivariate analysis, FBT infection was only related to the consumption of raw fish and gender. Seventy stool samples with a sufficient amount of faecal matter were subjected to DNA extraction, 42.85% of them yielded DNA production, and all were of Clonorchis sinensis. Conclusion. Results of the study showed the high prevalence of infection of fish-borne trematode, mostly C. sinensis among humans in Ninh Binh province. The prevention of FBT should be strengthened with programs detailed according to the distribution of FBT in different endemic areas. Hindawi 2019-07-30 /pmc/articles/PMC6699318/ /pubmed/31467915 http://dx.doi.org/10.1155/2019/8581379 Text en Copyright © 2019 Anh Kieu Thi Tran et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tran, Anh Kieu Thi
Doan, Hoa Thuy
Do, Anh Ngoc
Nguyen, Van Thi
Hoang, Su Xuan
Le, Huong Thu Thi
Hoang, Hoa Thi
Le, Nam Hoang
Le, Quyen Bao Thi
Le, Tran-Anh
Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
title Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
title_full Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
title_fullStr Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
title_full_unstemmed Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
title_short Prevalence, Species Distribution, and Related Factors of Fish-Borne Trematode Infection in Ninh Binh Province, Vietnam
title_sort prevalence, species distribution, and related factors of fish-borne trematode infection in ninh binh province, vietnam
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6699318/
https://www.ncbi.nlm.nih.gov/pubmed/31467915
http://dx.doi.org/10.1155/2019/8581379
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