Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera)

Novel proanthocyanidin fractions from grape stem extracts were purified using Amberlite XAD-1180N, Sephadex-LH-20, Toyopearl HW40F and reverse phase high-performance liquid chromatography. Two key compounds were estimated as epigallocatechin-(epicatechin)(7) gallate using electron-spray ionization t...

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Detalles Bibliográficos
Autores principales: Sei-ichi, Kawahara, Toda, Kazuya, Matsumoto, Kiriko, Ishihara, Chisato, Nonobe, Shuhei, Matsunaga, Chisato, Gomi, Yoshika K., Senga, Shogo, Kawaguchi, Koichiro, Yamamoto, Ayaka, Suwannachot, Jutalak, Noda, Reiko, Kobayashi, Shuhei, Hamauzu, Yasunori, Makabe, Hidefumi, Fujii, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700121/
https://www.ncbi.nlm.nih.gov/pubmed/31427660
http://dx.doi.org/10.1038/s41598-019-48603-5
Descripción
Sumario:Novel proanthocyanidin fractions from grape stem extracts were purified using Amberlite XAD-1180N, Sephadex-LH-20, Toyopearl HW40F and reverse phase high-performance liquid chromatography. Two key compounds were estimated as epigallocatechin-(epicatechin)(7) gallate using electron-spray ionization time-of-flight mass spectrometry. Epigallocatechin-(epicatechin)(7) gallate (compound 1) showed significant anti-cancer activity in PC-3 prostate cancer cells. In particular, compound 1 suppressed the gene expression of fatty acid-binding protein 5 (FABP5), which is involved in promoting cell proliferation and metastasis in prostate cancer cells.

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