Cargando…
Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera)
Novel proanthocyanidin fractions from grape stem extracts were purified using Amberlite XAD-1180N, Sephadex-LH-20, Toyopearl HW40F and reverse phase high-performance liquid chromatography. Two key compounds were estimated as epigallocatechin-(epicatechin)(7) gallate using electron-spray ionization t...
| Autores principales: | , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700121/ https://www.ncbi.nlm.nih.gov/pubmed/31427660 http://dx.doi.org/10.1038/s41598-019-48603-5 |
| _version_ | 1783444801222344704 |
|---|---|
| author | Sei-ichi, Kawahara Toda, Kazuya Matsumoto, Kiriko Ishihara, Chisato Nonobe, Shuhei Matsunaga, Chisato Gomi, Yoshika K. Senga, Shogo Kawaguchi, Koichiro Yamamoto, Ayaka Suwannachot, Jutalak Noda, Reiko Kobayashi, Shuhei Hamauzu, Yasunori Makabe, Hidefumi Fujii, Hiroshi |
| author_facet | Sei-ichi, Kawahara Toda, Kazuya Matsumoto, Kiriko Ishihara, Chisato Nonobe, Shuhei Matsunaga, Chisato Gomi, Yoshika K. Senga, Shogo Kawaguchi, Koichiro Yamamoto, Ayaka Suwannachot, Jutalak Noda, Reiko Kobayashi, Shuhei Hamauzu, Yasunori Makabe, Hidefumi Fujii, Hiroshi |
| author_sort | Sei-ichi, Kawahara |
| collection | PubMed |
| description | Novel proanthocyanidin fractions from grape stem extracts were purified using Amberlite XAD-1180N, Sephadex-LH-20, Toyopearl HW40F and reverse phase high-performance liquid chromatography. Two key compounds were estimated as epigallocatechin-(epicatechin)(7) gallate using electron-spray ionization time-of-flight mass spectrometry. Epigallocatechin-(epicatechin)(7) gallate (compound 1) showed significant anti-cancer activity in PC-3 prostate cancer cells. In particular, compound 1 suppressed the gene expression of fatty acid-binding protein 5 (FABP5), which is involved in promoting cell proliferation and metastasis in prostate cancer cells. |
| format | Online Article Text |
| id | pubmed-6700121 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-67001212019-08-21 Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera) Sei-ichi, Kawahara Toda, Kazuya Matsumoto, Kiriko Ishihara, Chisato Nonobe, Shuhei Matsunaga, Chisato Gomi, Yoshika K. Senga, Shogo Kawaguchi, Koichiro Yamamoto, Ayaka Suwannachot, Jutalak Noda, Reiko Kobayashi, Shuhei Hamauzu, Yasunori Makabe, Hidefumi Fujii, Hiroshi Sci Rep Article Novel proanthocyanidin fractions from grape stem extracts were purified using Amberlite XAD-1180N, Sephadex-LH-20, Toyopearl HW40F and reverse phase high-performance liquid chromatography. Two key compounds were estimated as epigallocatechin-(epicatechin)(7) gallate using electron-spray ionization time-of-flight mass spectrometry. Epigallocatechin-(epicatechin)(7) gallate (compound 1) showed significant anti-cancer activity in PC-3 prostate cancer cells. In particular, compound 1 suppressed the gene expression of fatty acid-binding protein 5 (FABP5), which is involved in promoting cell proliferation and metastasis in prostate cancer cells. Nature Publishing Group UK 2019-08-19 /pmc/articles/PMC6700121/ /pubmed/31427660 http://dx.doi.org/10.1038/s41598-019-48603-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Sei-ichi, Kawahara Toda, Kazuya Matsumoto, Kiriko Ishihara, Chisato Nonobe, Shuhei Matsunaga, Chisato Gomi, Yoshika K. Senga, Shogo Kawaguchi, Koichiro Yamamoto, Ayaka Suwannachot, Jutalak Noda, Reiko Kobayashi, Shuhei Hamauzu, Yasunori Makabe, Hidefumi Fujii, Hiroshi Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera) |
| title | Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera) |
| title_full | Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera) |
| title_fullStr | Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera) |
| title_full_unstemmed | Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera) |
| title_short | Isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (Vitis vinifera) |
| title_sort | isolation and characterization of a novel oligomeric proanthocyanidin with significant anti-cancer activities from grape stems (vitis vinifera) |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700121/ https://www.ncbi.nlm.nih.gov/pubmed/31427660 http://dx.doi.org/10.1038/s41598-019-48603-5 |
| work_keys_str_mv | AT seiichikawahara isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT todakazuya isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT matsumotokiriko isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT ishiharachisato isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT nonobeshuhei isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT matsunagachisato isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT gomiyoshikak isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT sengashogo isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT kawaguchikoichiro isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT yamamotoayaka isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT suwannachotjutalak isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT nodareiko isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT kobayashishuhei isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT hamauzuyasunori isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT makabehidefumi isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera AT fujiihiroshi isolationandcharacterizationofanoveloligomericproanthocyanidinwithsignificantanticanceractivitiesfromgrapestemsvitisvinifera |