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Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser

Low-Level Laser Therapy is used as regenerative therapy in different clinical fields. This is due to its photobiomodulation effect via cell signaling on different cell populations, Including fibroblasts, cells involved in tissue regeneration and healing. The aim was to analyze the effect of 940 nm d...

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Autores principales: Illescas-Montes, Rebeca, Melguizo-Rodríguez, Lucía, García-Martínez, Olga, de Luna-Bertos, Elvira, Manzano-Moreno, Francisco Javier, Ruiz, Concepción, Ramos-Torrecillas, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700136/
https://www.ncbi.nlm.nih.gov/pubmed/31427686
http://dx.doi.org/10.1038/s41598-019-48595-2
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author Illescas-Montes, Rebeca
Melguizo-Rodríguez, Lucía
García-Martínez, Olga
de Luna-Bertos, Elvira
Manzano-Moreno, Francisco Javier
Ruiz, Concepción
Ramos-Torrecillas, Javier
author_facet Illescas-Montes, Rebeca
Melguizo-Rodríguez, Lucía
García-Martínez, Olga
de Luna-Bertos, Elvira
Manzano-Moreno, Francisco Javier
Ruiz, Concepción
Ramos-Torrecillas, Javier
author_sort Illescas-Montes, Rebeca
collection PubMed
description Low-Level Laser Therapy is used as regenerative therapy in different clinical fields. This is due to its photobiomodulation effect via cell signaling on different cell populations, Including fibroblasts, cells involved in tissue regeneration and healing. The aim was to analyze the effect of 940 nm diode laser on the gene expression of different markers involved in fibroblast growth, differentiation, and migration. Real-time polymerase chain reaction (q-RT-PCR) was used to quantify the expression of fibroblast growth factor (FGF), connective tissue growth factor (CTGF), vascular-endothelial growth factor (VEGF), transforming growth factor β1 (TGF-β1), TGFβ-receptors (TGFβR1, TGFβR2, and TGFβR3), discoidin-domain receptor-2 (DDR2), matrix metalloproteinase-2 (MMP2), α-actin, fibronectin, decorin, and elastin on human fibroblast, treated with single dose (T1) or two doses (T2) of diode laser at 0.5 Watts and 4 J/cm(2). A significant increase in the expression of FGF, TGF-β1, TGFβR1, TGFβR2, α-actin, fibronectin, decorin, DDR2 and MMP2 was observed after both treatments. A decrease was observed in expression of elastin (T1 and T2), and CTGF (T2). These changes underlie the biostimulatory effect of laser on fibroblasts, which translates into an increase in short-term proliferation and in long-term differentiation to myofibroblasts. These data support the therapeutic potential of diode laser for wound repair.
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spelling pubmed-67001362019-08-21 Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser Illescas-Montes, Rebeca Melguizo-Rodríguez, Lucía García-Martínez, Olga de Luna-Bertos, Elvira Manzano-Moreno, Francisco Javier Ruiz, Concepción Ramos-Torrecillas, Javier Sci Rep Article Low-Level Laser Therapy is used as regenerative therapy in different clinical fields. This is due to its photobiomodulation effect via cell signaling on different cell populations, Including fibroblasts, cells involved in tissue regeneration and healing. The aim was to analyze the effect of 940 nm diode laser on the gene expression of different markers involved in fibroblast growth, differentiation, and migration. Real-time polymerase chain reaction (q-RT-PCR) was used to quantify the expression of fibroblast growth factor (FGF), connective tissue growth factor (CTGF), vascular-endothelial growth factor (VEGF), transforming growth factor β1 (TGF-β1), TGFβ-receptors (TGFβR1, TGFβR2, and TGFβR3), discoidin-domain receptor-2 (DDR2), matrix metalloproteinase-2 (MMP2), α-actin, fibronectin, decorin, and elastin on human fibroblast, treated with single dose (T1) or two doses (T2) of diode laser at 0.5 Watts and 4 J/cm(2). A significant increase in the expression of FGF, TGF-β1, TGFβR1, TGFβR2, α-actin, fibronectin, decorin, DDR2 and MMP2 was observed after both treatments. A decrease was observed in expression of elastin (T1 and T2), and CTGF (T2). These changes underlie the biostimulatory effect of laser on fibroblasts, which translates into an increase in short-term proliferation and in long-term differentiation to myofibroblasts. These data support the therapeutic potential of diode laser for wound repair. Nature Publishing Group UK 2019-08-19 /pmc/articles/PMC6700136/ /pubmed/31427686 http://dx.doi.org/10.1038/s41598-019-48595-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Illescas-Montes, Rebeca
Melguizo-Rodríguez, Lucía
García-Martínez, Olga
de Luna-Bertos, Elvira
Manzano-Moreno, Francisco Javier
Ruiz, Concepción
Ramos-Torrecillas, Javier
Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser
title Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser
title_full Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser
title_fullStr Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser
title_full_unstemmed Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser
title_short Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser
title_sort human fibroblast gene expression modulation using 940 nm diode laser
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700136/
https://www.ncbi.nlm.nih.gov/pubmed/31427686
http://dx.doi.org/10.1038/s41598-019-48595-2
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