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Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis
Humic substances have been widely used as plant growth promoters to improve the yield of agricultural crops. However, the mechanisms underlying this effect remain unclear. Root soluble protein profiles in plants 11 days after planting and cultivated with and without humic acids (HA, 50 mg CL(−1)), w...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700139/ https://www.ncbi.nlm.nih.gov/pubmed/31427667 http://dx.doi.org/10.1038/s41598-019-48509-2 |
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author | Nunes, Rosane Oliveira Domiciano, Giselli Abrahão Alves, Wilber Sousa Melo, Ana Claudia Amaral Nogueira, Fábio Cesar Sousa Canellas, Luciano Pasqualoto Olivares, Fábio Lopes Zingali, Russolina Benedeta Soares, Márcia Regina |
author_facet | Nunes, Rosane Oliveira Domiciano, Giselli Abrahão Alves, Wilber Sousa Melo, Ana Claudia Amaral Nogueira, Fábio Cesar Sousa Canellas, Luciano Pasqualoto Olivares, Fábio Lopes Zingali, Russolina Benedeta Soares, Márcia Regina |
author_sort | Nunes, Rosane Oliveira |
collection | PubMed |
description | Humic substances have been widely used as plant growth promoters to improve the yield of agricultural crops. However, the mechanisms underlying this effect remain unclear. Root soluble protein profiles in plants 11 days after planting and cultivated with and without humic acids (HA, 50 mg CL(−1)), were analyzed using the label-free quantitative proteomic approach. Cultivation of maize with HA resulted in higher fresh weight of roots than in untreated plants (control). Plants treated with HA showed increased number, diameter and length of roots. In the proteomics analysis, differences were detected in the following categories: energy metabolism, cytoskeleton, cellular transport, conformation and degradation of proteins, and DNA replication. Thirty-four proteins were significantly more abundant in the seedlings treated with HA, whereas only nine proteins were abundant in the control. The effects on root architecture, such as the induction of lateral roots and biomass increase were accompanied by changes in the energy metabolism-associated proteins. The results show that the main effect of HA is protective, mainly associated with increased expression of the 2-cys peroxidase, putative VHS/GAT, and glutathione proteins. Indeed, these proteins had the highest fold-difference. Overall, these results improve our understanding of the molecular mechanisms of HA-promoted plant growth. |
format | Online Article Text |
id | pubmed-6700139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67001392019-08-21 Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis Nunes, Rosane Oliveira Domiciano, Giselli Abrahão Alves, Wilber Sousa Melo, Ana Claudia Amaral Nogueira, Fábio Cesar Sousa Canellas, Luciano Pasqualoto Olivares, Fábio Lopes Zingali, Russolina Benedeta Soares, Márcia Regina Sci Rep Article Humic substances have been widely used as plant growth promoters to improve the yield of agricultural crops. However, the mechanisms underlying this effect remain unclear. Root soluble protein profiles in plants 11 days after planting and cultivated with and without humic acids (HA, 50 mg CL(−1)), were analyzed using the label-free quantitative proteomic approach. Cultivation of maize with HA resulted in higher fresh weight of roots than in untreated plants (control). Plants treated with HA showed increased number, diameter and length of roots. In the proteomics analysis, differences were detected in the following categories: energy metabolism, cytoskeleton, cellular transport, conformation and degradation of proteins, and DNA replication. Thirty-four proteins were significantly more abundant in the seedlings treated with HA, whereas only nine proteins were abundant in the control. The effects on root architecture, such as the induction of lateral roots and biomass increase were accompanied by changes in the energy metabolism-associated proteins. The results show that the main effect of HA is protective, mainly associated with increased expression of the 2-cys peroxidase, putative VHS/GAT, and glutathione proteins. Indeed, these proteins had the highest fold-difference. Overall, these results improve our understanding of the molecular mechanisms of HA-promoted plant growth. Nature Publishing Group UK 2019-08-19 /pmc/articles/PMC6700139/ /pubmed/31427667 http://dx.doi.org/10.1038/s41598-019-48509-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nunes, Rosane Oliveira Domiciano, Giselli Abrahão Alves, Wilber Sousa Melo, Ana Claudia Amaral Nogueira, Fábio Cesar Sousa Canellas, Luciano Pasqualoto Olivares, Fábio Lopes Zingali, Russolina Benedeta Soares, Márcia Regina Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis |
title | Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis |
title_full | Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis |
title_fullStr | Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis |
title_full_unstemmed | Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis |
title_short | Evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis |
title_sort | evaluation of the effects of humic acids on maize root architecture by label-free proteomics analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700139/ https://www.ncbi.nlm.nih.gov/pubmed/31427667 http://dx.doi.org/10.1038/s41598-019-48509-2 |
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