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Bacillus megaterium SF185 spores exert protective effects against oxidative stress in vivo and in vitro
Endogenous reactive oxygen species (ROS) are by-products of the aerobic metabolism of cells and have an important signalling role as secondary messengers in various physiological processes, including cell growth and development. However, the excessive production of ROS, as well as the exposure to ex...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700169/ https://www.ncbi.nlm.nih.gov/pubmed/31427655 http://dx.doi.org/10.1038/s41598-019-48531-4 |
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author | Mazzoli, Arianna Donadio, Giuliana Lanzilli, Mariamichela Saggese, Anella Guarino, Andrea Maria Rivetti, Miriam Crescenzo, Raffaella Ricca, Ezio Ferrandino, Ida Iossa, Susanna Pollice, Alessandra Isticato, Rachele |
author_facet | Mazzoli, Arianna Donadio, Giuliana Lanzilli, Mariamichela Saggese, Anella Guarino, Andrea Maria Rivetti, Miriam Crescenzo, Raffaella Ricca, Ezio Ferrandino, Ida Iossa, Susanna Pollice, Alessandra Isticato, Rachele |
author_sort | Mazzoli, Arianna |
collection | PubMed |
description | Endogenous reactive oxygen species (ROS) are by-products of the aerobic metabolism of cells and have an important signalling role as secondary messengers in various physiological processes, including cell growth and development. However, the excessive production of ROS, as well as the exposure to exogenous ROS, can cause protein oxidation, lipid peroxidation and DNA damages leading to cell injuries. ROS accumulation has been associated to the development of health disorders such as neurodegenerative and cardiovascular diseases, inflammatory bowel disease and cancer. We report that spores of strain SF185, a human isolate of Bacillus megaterium, have antioxidant activity on Caco-2 cells exposed to hydrogen peroxide and on a murine model of dextran sodium sulfate-induced oxidative stress. In both model systems spores exert a protective state due to their scavenging action: on cells, spores reduce the amount of intracellular ROS, while in vivo the pre-treatment with spores protects mice from the chemically-induced damages. Overall, our results suggest that treatment with SF185 spores prevents or reduces the damages caused by oxidative stress. The human origin of SF185, its strong antioxidant activity, and its protective effects led to propose the spore of this strain as a new probiotic for gut health. |
format | Online Article Text |
id | pubmed-6700169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67001692019-08-21 Bacillus megaterium SF185 spores exert protective effects against oxidative stress in vivo and in vitro Mazzoli, Arianna Donadio, Giuliana Lanzilli, Mariamichela Saggese, Anella Guarino, Andrea Maria Rivetti, Miriam Crescenzo, Raffaella Ricca, Ezio Ferrandino, Ida Iossa, Susanna Pollice, Alessandra Isticato, Rachele Sci Rep Article Endogenous reactive oxygen species (ROS) are by-products of the aerobic metabolism of cells and have an important signalling role as secondary messengers in various physiological processes, including cell growth and development. However, the excessive production of ROS, as well as the exposure to exogenous ROS, can cause protein oxidation, lipid peroxidation and DNA damages leading to cell injuries. ROS accumulation has been associated to the development of health disorders such as neurodegenerative and cardiovascular diseases, inflammatory bowel disease and cancer. We report that spores of strain SF185, a human isolate of Bacillus megaterium, have antioxidant activity on Caco-2 cells exposed to hydrogen peroxide and on a murine model of dextran sodium sulfate-induced oxidative stress. In both model systems spores exert a protective state due to their scavenging action: on cells, spores reduce the amount of intracellular ROS, while in vivo the pre-treatment with spores protects mice from the chemically-induced damages. Overall, our results suggest that treatment with SF185 spores prevents or reduces the damages caused by oxidative stress. The human origin of SF185, its strong antioxidant activity, and its protective effects led to propose the spore of this strain as a new probiotic for gut health. Nature Publishing Group UK 2019-08-19 /pmc/articles/PMC6700169/ /pubmed/31427655 http://dx.doi.org/10.1038/s41598-019-48531-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Mazzoli, Arianna Donadio, Giuliana Lanzilli, Mariamichela Saggese, Anella Guarino, Andrea Maria Rivetti, Miriam Crescenzo, Raffaella Ricca, Ezio Ferrandino, Ida Iossa, Susanna Pollice, Alessandra Isticato, Rachele Bacillus megaterium SF185 spores exert protective effects against oxidative stress in vivo and in vitro |
title | Bacillus megaterium SF185 spores exert protective effects against oxidative stress in vivo and in vitro |
title_full | Bacillus megaterium SF185 spores exert protective effects against oxidative stress in vivo and in vitro |
title_fullStr | Bacillus megaterium SF185 spores exert protective effects against oxidative stress in vivo and in vitro |
title_full_unstemmed | Bacillus megaterium SF185 spores exert protective effects against oxidative stress in vivo and in vitro |
title_short | Bacillus megaterium SF185 spores exert protective effects against oxidative stress in vivo and in vitro |
title_sort | bacillus megaterium sf185 spores exert protective effects against oxidative stress in vivo and in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700169/ https://www.ncbi.nlm.nih.gov/pubmed/31427655 http://dx.doi.org/10.1038/s41598-019-48531-4 |
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