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Colletotrichum Species Associated with Japanese Plum (Prunus salicina) Anthracnose in South Korea

A total of 24 Colletotrichum isolates were isolated from diseased Japanese plum (Prunus salicina) fruits showing chlorotic regions with whitish-brown sunken necrotic lesions and phylogenetic relationships among the collected Colletotrichum isolates were determined. A subset of 11 isolates was chosen...

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Autores principales: Hassan, Oliul, Lee, Yong Se, Chang, Taehyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700192/
https://www.ncbi.nlm.nih.gov/pubmed/31427596
http://dx.doi.org/10.1038/s41598-019-48108-1
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author Hassan, Oliul
Lee, Yong Se
Chang, Taehyun
author_facet Hassan, Oliul
Lee, Yong Se
Chang, Taehyun
author_sort Hassan, Oliul
collection PubMed
description A total of 24 Colletotrichum isolates were isolated from diseased Japanese plum (Prunus salicina) fruits showing chlorotic regions with whitish-brown sunken necrotic lesions and phylogenetic relationships among the collected Colletotrichum isolates were determined. A subset of 11 isolates was chosen for further taxonomic study based on morphology and molecular characteristics identified using the internal transcribed spacer (ITS) and beta-tubulin (TUB2) genes. Isolates in the C. acutatum complex were analyzed using partial sequencing of five gene regions (ITS, GAPDH, ACT, TUB2, and CHS), and C. gloeosporioides sensu lato (s.l.) isolates were analyzed using seven gene regions (ITS, TUB2, GAPDH, ACT, CAL, CHS-1, and ApMat). Morphological assessments in combination with phylogenetic analysis delineated four species of Colletotrichum including C. gloeosporioides sensu stricto (s.s.), C. nymphaeae, C. foriniae, and C. siamense; these data identify Colletotrichum fioriniae and C. siamense two new species associated with plum anthracnose in South Korea. Finally, the pathogenicity of these four species in the development of plum anthracnose in South Korea was confirmed by inoculations of plum fruit.
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spelling pubmed-67001922019-08-21 Colletotrichum Species Associated with Japanese Plum (Prunus salicina) Anthracnose in South Korea Hassan, Oliul Lee, Yong Se Chang, Taehyun Sci Rep Article A total of 24 Colletotrichum isolates were isolated from diseased Japanese plum (Prunus salicina) fruits showing chlorotic regions with whitish-brown sunken necrotic lesions and phylogenetic relationships among the collected Colletotrichum isolates were determined. A subset of 11 isolates was chosen for further taxonomic study based on morphology and molecular characteristics identified using the internal transcribed spacer (ITS) and beta-tubulin (TUB2) genes. Isolates in the C. acutatum complex were analyzed using partial sequencing of five gene regions (ITS, GAPDH, ACT, TUB2, and CHS), and C. gloeosporioides sensu lato (s.l.) isolates were analyzed using seven gene regions (ITS, TUB2, GAPDH, ACT, CAL, CHS-1, and ApMat). Morphological assessments in combination with phylogenetic analysis delineated four species of Colletotrichum including C. gloeosporioides sensu stricto (s.s.), C. nymphaeae, C. foriniae, and C. siamense; these data identify Colletotrichum fioriniae and C. siamense two new species associated with plum anthracnose in South Korea. Finally, the pathogenicity of these four species in the development of plum anthracnose in South Korea was confirmed by inoculations of plum fruit. Nature Publishing Group UK 2019-08-19 /pmc/articles/PMC6700192/ /pubmed/31427596 http://dx.doi.org/10.1038/s41598-019-48108-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hassan, Oliul
Lee, Yong Se
Chang, Taehyun
Colletotrichum Species Associated with Japanese Plum (Prunus salicina) Anthracnose in South Korea
title Colletotrichum Species Associated with Japanese Plum (Prunus salicina) Anthracnose in South Korea
title_full Colletotrichum Species Associated with Japanese Plum (Prunus salicina) Anthracnose in South Korea
title_fullStr Colletotrichum Species Associated with Japanese Plum (Prunus salicina) Anthracnose in South Korea
title_full_unstemmed Colletotrichum Species Associated with Japanese Plum (Prunus salicina) Anthracnose in South Korea
title_short Colletotrichum Species Associated with Japanese Plum (Prunus salicina) Anthracnose in South Korea
title_sort colletotrichum species associated with japanese plum (prunus salicina) anthracnose in south korea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700192/
https://www.ncbi.nlm.nih.gov/pubmed/31427596
http://dx.doi.org/10.1038/s41598-019-48108-1
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