ShadowR: a novel chromoprotein with reduced non-specific binding and improved expression in living cells

Here we developed an orange light-absorbing chromoprotein named ShadowR as a novel acceptor for performing fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) measurement in living cells. ShadowR was generated by replacing hydrophobic amino acids located at t...

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Detalles Bibliográficos
Autores principales: Murakoshi, Hideji, Horiuchi, Hiroshi, Kosugi, Takahiro, Onda, Maki, Sato, Aiko, Koga, Nobuyasu, Nabekura, Junichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700193/
https://www.ncbi.nlm.nih.gov/pubmed/31427680
http://dx.doi.org/10.1038/s41598-019-48604-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700193/
https://www.ncbi.nlm.nih.gov/pubmed/31427680
http://dx.doi.org/10.1038/s41598-019-48604-4

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