Cargando…

Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata)

Heavy metal accumulation in mesquite trees (Prosopis laevigata) growing in aluminum, titanium, chromium and zirconium-polluted soils of a semi-arid region in Mexico was investigated using wavelength dispersive X-ray fluorescence analysis. The results showed that P. laevigata trees can hyper accumula...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramírez, Verónica, Baez, Antonino, López, Primavera, Bustillos, Rocío, Villalobos, Miguel Ángel, Carreño, Ricardo, Contreras, José Luis, Muñoz-Rojas, Jesús, Fuentes, Luis Ernesto, Martínez, Javier, Munive, José Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700308/
https://www.ncbi.nlm.nih.gov/pubmed/31456770
http://dx.doi.org/10.3389/fmicb.2019.01833
_version_ 1783444845615906816
author Ramírez, Verónica
Baez, Antonino
López, Primavera
Bustillos, Rocío
Villalobos, Miguel Ángel
Carreño, Ricardo
Contreras, José Luis
Muñoz-Rojas, Jesús
Fuentes, Luis Ernesto
Martínez, Javier
Munive, José Antonio
author_facet Ramírez, Verónica
Baez, Antonino
López, Primavera
Bustillos, Rocío
Villalobos, Miguel Ángel
Carreño, Ricardo
Contreras, José Luis
Muñoz-Rojas, Jesús
Fuentes, Luis Ernesto
Martínez, Javier
Munive, José Antonio
author_sort Ramírez, Verónica
collection PubMed
description Heavy metal accumulation in mesquite trees (Prosopis laevigata) growing in aluminum, titanium, chromium and zirconium-polluted soils of a semi-arid region in Mexico was investigated using wavelength dispersive X-ray fluorescence analysis. The results showed that P. laevigata trees can hyper accumulate up to 4100 mg/kg of Al, 14000 mg/kg of Fe, 1600 mg/kg of Ti, 2500 mg/kg of Zn, but not chromium, regarding high chromium concentrations found in soils (435 mg/kg). Since plant-associated microorganism can modulate phytoremediation efficiency, the biodiversity of P. laevigata associated bacteria was studied. Eighty-eight isolates from P. laevigata nodules were obtained; all isolates tolerated high concentrations of Al, Fe, Zn and Cr in vitro. The top-six chromium tolerant strains were identified by 16S rRNA sequence analysis as belonging to genus Bacillus. Bacillus sp. MH778713, close to Bacillus cereus group, showed to be the most resistant strain, tolerating up to 15000 mg/L Cr (VI) and 10000 mg/L of Al. Regarding the bioaccumulation traits, Bacillus sp. MH778713 accumulated up to 100 mg Cr(VI)/g of cells when it was exposed to 1474 mg/L of Cr VI. To assess Bacillus sp. MH778713 ability to assist Prosopis laevigata phytoremediation; twenty plants were inoculated or non-inoculated with Bacillus sp. MH778713 and grown in nitrogen-free Jensen’s medium added with 0, 10 and 25 mg/L of Cr(VI). Only plants inoculated with Bacillus sp. grew in the presence of chromium showing the ability of this strain to assist chromium phytoremediation. P. laevigata and Bacillus spp. may be considered as good candidates for soil restoration of arid and semiarid sites contaminated with heavy metals.
format Online
Article
Text
id pubmed-6700308
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-67003082019-08-27 Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata) Ramírez, Verónica Baez, Antonino López, Primavera Bustillos, Rocío Villalobos, Miguel Ángel Carreño, Ricardo Contreras, José Luis Muñoz-Rojas, Jesús Fuentes, Luis Ernesto Martínez, Javier Munive, José Antonio Front Microbiol Microbiology Heavy metal accumulation in mesquite trees (Prosopis laevigata) growing in aluminum, titanium, chromium and zirconium-polluted soils of a semi-arid region in Mexico was investigated using wavelength dispersive X-ray fluorescence analysis. The results showed that P. laevigata trees can hyper accumulate up to 4100 mg/kg of Al, 14000 mg/kg of Fe, 1600 mg/kg of Ti, 2500 mg/kg of Zn, but not chromium, regarding high chromium concentrations found in soils (435 mg/kg). Since plant-associated microorganism can modulate phytoremediation efficiency, the biodiversity of P. laevigata associated bacteria was studied. Eighty-eight isolates from P. laevigata nodules were obtained; all isolates tolerated high concentrations of Al, Fe, Zn and Cr in vitro. The top-six chromium tolerant strains were identified by 16S rRNA sequence analysis as belonging to genus Bacillus. Bacillus sp. MH778713, close to Bacillus cereus group, showed to be the most resistant strain, tolerating up to 15000 mg/L Cr (VI) and 10000 mg/L of Al. Regarding the bioaccumulation traits, Bacillus sp. MH778713 accumulated up to 100 mg Cr(VI)/g of cells when it was exposed to 1474 mg/L of Cr VI. To assess Bacillus sp. MH778713 ability to assist Prosopis laevigata phytoremediation; twenty plants were inoculated or non-inoculated with Bacillus sp. MH778713 and grown in nitrogen-free Jensen’s medium added with 0, 10 and 25 mg/L of Cr(VI). Only plants inoculated with Bacillus sp. grew in the presence of chromium showing the ability of this strain to assist chromium phytoremediation. P. laevigata and Bacillus spp. may be considered as good candidates for soil restoration of arid and semiarid sites contaminated with heavy metals. Frontiers Media S.A. 2019-08-13 /pmc/articles/PMC6700308/ /pubmed/31456770 http://dx.doi.org/10.3389/fmicb.2019.01833 Text en Copyright © 2019 Ramírez, Baez, López, Bustillos, Villalobos, Carreño, Contreras, Muñoz-Rojas, Fuentes, Martínez and Munive. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ramírez, Verónica
Baez, Antonino
López, Primavera
Bustillos, Rocío
Villalobos, Miguel Ángel
Carreño, Ricardo
Contreras, José Luis
Muñoz-Rojas, Jesús
Fuentes, Luis Ernesto
Martínez, Javier
Munive, José Antonio
Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata)
title Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata)
title_full Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata)
title_fullStr Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata)
title_full_unstemmed Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata)
title_short Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata)
title_sort chromium hyper-tolerant bacillus sp. mh778713 assists phytoremediation of heavy metals by mesquite trees (prosopis laevigata)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700308/
https://www.ncbi.nlm.nih.gov/pubmed/31456770
http://dx.doi.org/10.3389/fmicb.2019.01833
work_keys_str_mv AT ramirezveronica chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT baezantonino chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT lopezprimavera chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT bustillosrocio chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT villalobosmiguelangel chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT carrenoricardo chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT contrerasjoseluis chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT munozrojasjesus chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT fuentesluisernesto chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT martinezjavier chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata
AT munivejoseantonio chromiumhypertolerantbacillusspmh778713assistsphytoremediationofheavymetalsbymesquitetreesprosopislaevigata