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Analysis of the length polymorphisms in sequence-tagged-site sY1291 on Y chromosome in Vietnamese men of infertile couples
This study aims to analyze the length polymorphisms in sequence-tagged-site (STS) sY1291 of the Y chromosome in Vietnamese men of infertile couples. All 322 DNA samples were amplified with the sY1291 primer by the quantitative fluorescent polymerase chain reaction (QF-PCR) assay. DNA sequencing tech...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700317/ https://www.ncbi.nlm.nih.gov/pubmed/31427585 http://dx.doi.org/10.1038/s41598-019-45649-3 |
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author | Cao, Nguyen Thi Tai Nguyen, Kien Trung Vu, Nhuan Thi Nguyen, Vieng Chung Trinh, Thiet Minh Nguyen, Ngoc Thi Bich Trinh, Lien Thi Bich Lam, Tien Thi Thuy Cao, Binh Luong Dang, Tra Ngoc |
author_facet | Cao, Nguyen Thi Tai Nguyen, Kien Trung Vu, Nhuan Thi Nguyen, Vieng Chung Trinh, Thiet Minh Nguyen, Ngoc Thi Bich Trinh, Lien Thi Bich Lam, Tien Thi Thuy Cao, Binh Luong Dang, Tra Ngoc |
author_sort | Cao, Nguyen Thi Tai |
collection | PubMed |
description | This study aims to analyze the length polymorphisms in sequence-tagged-site (STS) sY1291 of the Y chromosome in Vietnamese men of infertile couples. All 322 DNA samples were amplified with the sY1291 primer by the quantitative fluorescent polymerase chain reaction (QF-PCR) assay. DNA sequencing technique was employed to evaluate the accuracy of QF-PCR results. The study showed 273 out of 322 DNA samples had the presence of STS sY1291, accounted for 84.78%. The QF-PCR results showed that there were various lengths in STS sY1291: 507 bp, 512 bp, 523 bp and 527 bp. The most prevalent length in STS sY1291 was 507 bp (87.5%), the others were 512 bp (4.8%), 523 bp (4.8%) and 527 bp (2.9%). We found that the observed length polymorphisms derived from differences in the number of mononucleotide Thymine (T) repeats in its structure. It stretched from 22 T to 39 T. DNA sequencing results identified that the number of mononucleotide T repeats causes these polymorphisms. However, the pair-wise alignment between the obtained and reference sequence was 77%. It can be seen that the length polymorphisms in STS sY1291 observed in QF-PCR results was accurate but it is still difficult to sequence fragments with mononucleotide repeats. |
format | Online Article Text |
id | pubmed-6700317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67003172019-08-21 Analysis of the length polymorphisms in sequence-tagged-site sY1291 on Y chromosome in Vietnamese men of infertile couples Cao, Nguyen Thi Tai Nguyen, Kien Trung Vu, Nhuan Thi Nguyen, Vieng Chung Trinh, Thiet Minh Nguyen, Ngoc Thi Bich Trinh, Lien Thi Bich Lam, Tien Thi Thuy Cao, Binh Luong Dang, Tra Ngoc Sci Rep Article This study aims to analyze the length polymorphisms in sequence-tagged-site (STS) sY1291 of the Y chromosome in Vietnamese men of infertile couples. All 322 DNA samples were amplified with the sY1291 primer by the quantitative fluorescent polymerase chain reaction (QF-PCR) assay. DNA sequencing technique was employed to evaluate the accuracy of QF-PCR results. The study showed 273 out of 322 DNA samples had the presence of STS sY1291, accounted for 84.78%. The QF-PCR results showed that there were various lengths in STS sY1291: 507 bp, 512 bp, 523 bp and 527 bp. The most prevalent length in STS sY1291 was 507 bp (87.5%), the others were 512 bp (4.8%), 523 bp (4.8%) and 527 bp (2.9%). We found that the observed length polymorphisms derived from differences in the number of mononucleotide Thymine (T) repeats in its structure. It stretched from 22 T to 39 T. DNA sequencing results identified that the number of mononucleotide T repeats causes these polymorphisms. However, the pair-wise alignment between the obtained and reference sequence was 77%. It can be seen that the length polymorphisms in STS sY1291 observed in QF-PCR results was accurate but it is still difficult to sequence fragments with mononucleotide repeats. Nature Publishing Group UK 2019-08-20 /pmc/articles/PMC6700317/ /pubmed/31427585 http://dx.doi.org/10.1038/s41598-019-45649-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Cao, Nguyen Thi Tai Nguyen, Kien Trung Vu, Nhuan Thi Nguyen, Vieng Chung Trinh, Thiet Minh Nguyen, Ngoc Thi Bich Trinh, Lien Thi Bich Lam, Tien Thi Thuy Cao, Binh Luong Dang, Tra Ngoc Analysis of the length polymorphisms in sequence-tagged-site sY1291 on Y chromosome in Vietnamese men of infertile couples |
title | Analysis of the length polymorphisms in sequence-tagged-site sY1291 on Y chromosome in Vietnamese men of infertile couples |
title_full | Analysis of the length polymorphisms in sequence-tagged-site sY1291 on Y chromosome in Vietnamese men of infertile couples |
title_fullStr | Analysis of the length polymorphisms in sequence-tagged-site sY1291 on Y chromosome in Vietnamese men of infertile couples |
title_full_unstemmed | Analysis of the length polymorphisms in sequence-tagged-site sY1291 on Y chromosome in Vietnamese men of infertile couples |
title_short | Analysis of the length polymorphisms in sequence-tagged-site sY1291 on Y chromosome in Vietnamese men of infertile couples |
title_sort | analysis of the length polymorphisms in sequence-tagged-site sy1291 on y chromosome in vietnamese men of infertile couples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700317/ https://www.ncbi.nlm.nih.gov/pubmed/31427585 http://dx.doi.org/10.1038/s41598-019-45649-3 |
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