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Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples
Hepatocellular carcinoma (HCC) is an extremely fatal malignancy. Intestinal microRNAs, which can be detected in fecal samples in humans may be involved in the pathological process of HCC. Therefore, screening for functional intestinal microRNAs in fecal samples and investigating their potential role...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700738/ https://www.ncbi.nlm.nih.gov/pubmed/31456816 http://dx.doi.org/10.3389/fgene.2019.00687 |
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author | Wang, Hui Lv, Yuan Wang, Cao Leng, Dongjing Yan, Yan Blessing Fasae, Moyondafoluwa Madiha Zahra, Syeda Jiang, Yanan Wang, Zhiguo Yang, Baofeng Bai, Yunlong |
author_facet | Wang, Hui Lv, Yuan Wang, Cao Leng, Dongjing Yan, Yan Blessing Fasae, Moyondafoluwa Madiha Zahra, Syeda Jiang, Yanan Wang, Zhiguo Yang, Baofeng Bai, Yunlong |
author_sort | Wang, Hui |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is an extremely fatal malignancy. Intestinal microRNAs, which can be detected in fecal samples in humans may be involved in the pathological process of HCC. Therefore, screening for functional intestinal microRNAs in fecal samples and investigating their potential roles in the molecular progression of HCC are necessary. Quantitative real-time PCR (qRT-PCR) has been widely used in microRNA expression studies. However, few genes have been reported as reference genes for intestinal microRNAs in fecal samples. In order to obtain a more accurately analyzed intestinal microRNAs expression, we first searched for reliable reference genes for intestinal microRNAs expression normalization during qRT-PCR, using three software packages (GeNorm, NormFinder, and Bestkeeper). Next we screened and predicted the target genes of the differentially intestinal microRNAs of control and HCC mice through quantitative RT-PCR or miRtarBase. Finally, we also analyzed the mRNA targets for enrichment of Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways using the DAVID Bioinformatic Resources database. This study has successfully screened relatively suitable reference genes and we have discovered that the differential intestinal microRNAs play significant roles in the development of HCC. The top reference genes identified in this study could provide a theoretical foundation for the reasonable selection of a suitable reference gene. Furthermore, the detection of intestinal microRNAs expression may serve as a promising therapeutic target for the diagnosis and treatment of HCC. |
format | Online Article Text |
id | pubmed-6700738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67007382019-08-27 Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples Wang, Hui Lv, Yuan Wang, Cao Leng, Dongjing Yan, Yan Blessing Fasae, Moyondafoluwa Madiha Zahra, Syeda Jiang, Yanan Wang, Zhiguo Yang, Baofeng Bai, Yunlong Front Genet Genetics Hepatocellular carcinoma (HCC) is an extremely fatal malignancy. Intestinal microRNAs, which can be detected in fecal samples in humans may be involved in the pathological process of HCC. Therefore, screening for functional intestinal microRNAs in fecal samples and investigating their potential roles in the molecular progression of HCC are necessary. Quantitative real-time PCR (qRT-PCR) has been widely used in microRNA expression studies. However, few genes have been reported as reference genes for intestinal microRNAs in fecal samples. In order to obtain a more accurately analyzed intestinal microRNAs expression, we first searched for reliable reference genes for intestinal microRNAs expression normalization during qRT-PCR, using three software packages (GeNorm, NormFinder, and Bestkeeper). Next we screened and predicted the target genes of the differentially intestinal microRNAs of control and HCC mice through quantitative RT-PCR or miRtarBase. Finally, we also analyzed the mRNA targets for enrichment of Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways using the DAVID Bioinformatic Resources database. This study has successfully screened relatively suitable reference genes and we have discovered that the differential intestinal microRNAs play significant roles in the development of HCC. The top reference genes identified in this study could provide a theoretical foundation for the reasonable selection of a suitable reference gene. Furthermore, the detection of intestinal microRNAs expression may serve as a promising therapeutic target for the diagnosis and treatment of HCC. Frontiers Media S.A. 2019-08-13 /pmc/articles/PMC6700738/ /pubmed/31456816 http://dx.doi.org/10.3389/fgene.2019.00687 Text en Copyright © 2019 Wang, Lv, Wang, Leng, Yan, Blessing Fasae, Madiha Zahra, Jiang, Wang, Yang and Bai http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Wang, Hui Lv, Yuan Wang, Cao Leng, Dongjing Yan, Yan Blessing Fasae, Moyondafoluwa Madiha Zahra, Syeda Jiang, Yanan Wang, Zhiguo Yang, Baofeng Bai, Yunlong Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples |
title | Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples |
title_full | Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples |
title_fullStr | Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples |
title_full_unstemmed | Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples |
title_short | Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples |
title_sort | systematic analysis of intestinal micrornas expression in hcc: identification of suitable reference genes in fecal samples |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700738/ https://www.ncbi.nlm.nih.gov/pubmed/31456816 http://dx.doi.org/10.3389/fgene.2019.00687 |
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