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Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep

BACKGROUND: Parallel in vitro and in vivo experiments were designed to evaluate promising chemotherapeutic alternatives for controlling haemonchosis in ruminants. In vitro anthelmintic activities (egg hatch test – EHT; larval development test – LDT) of aqueous and methanolic herbal extracts Mix1 and...

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Autores principales: Mravčáková, Dominika, Váradyová, Zora, Kopčáková, Anna, Čobanová, Klaudia, Grešáková, Ľubomíra, Kišidayová, Svetlana, Babják, Michal, Dolinská, Michaela Urda, Dvorožňáková, Emília, Königová, Alžbeta, Vadlejch, Jaroslav, Cieslak, Adam, Ślusarczyk, Sylwester, Várady, Marián
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700814/
https://www.ncbi.nlm.nih.gov/pubmed/31429752
http://dx.doi.org/10.1186/s12917-019-2050-2
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author Mravčáková, Dominika
Váradyová, Zora
Kopčáková, Anna
Čobanová, Klaudia
Grešáková, Ľubomíra
Kišidayová, Svetlana
Babják, Michal
Dolinská, Michaela Urda
Dvorožňáková, Emília
Königová, Alžbeta
Vadlejch, Jaroslav
Cieslak, Adam
Ślusarczyk, Sylwester
Várady, Marián
author_facet Mravčáková, Dominika
Váradyová, Zora
Kopčáková, Anna
Čobanová, Klaudia
Grešáková, Ľubomíra
Kišidayová, Svetlana
Babják, Michal
Dolinská, Michaela Urda
Dvorožňáková, Emília
Königová, Alžbeta
Vadlejch, Jaroslav
Cieslak, Adam
Ślusarczyk, Sylwester
Várady, Marián
author_sort Mravčáková, Dominika
collection PubMed
description BACKGROUND: Parallel in vitro and in vivo experiments were designed to evaluate promising chemotherapeutic alternatives for controlling haemonchosis in ruminants. In vitro anthelmintic activities (egg hatch test – EHT; larval development test – LDT) of aqueous and methanolic herbal extracts Mix1 and Mix2 were investigated. The in vivo effects of dietary supplementation with Mix1 and Mix2 on the parasitological status, inflammatory response, antioxidant parameters and microbial community of the lambs infected experimentally with Haemonchus contortus were investigated. Lambs were divided into four groups for the in vivo study: uninfected control lambs (C), infected lambs (I), infected lambs supplemented with Mix1 (I + Mix1) and infected lambs supplemented with Mix2 (I + Mix2). The experimental period was 70 days. RESULTS: The number of eggs per gram (EPG) of feces was quantified 22, 30, 37, 44, 51, 58, 65 and 70 days post-infection, and mean abomasal worm counts were assessed 70 days post-infection. Quantitative analyses identified 57.3 and 22.2 mg/g phenolic acids, 41.5 and 29.5 mg/g flavonoids and 1.4 and 1.33 mg/g protoberberine-type alkaloids in Mix1 and Mix2, respectively. The methanolic extracts of the herbal mixtures in both in vitro tests had higher anthelmintic effects (P < 0.01) than the aqueous extracts, but the effects did not differ significantly between Mix1 and Mix2 (P > 0.05). I + Mix1 and I + Mix2 lowered mean EPGs between 44 and 70 d by 58.1 and 51.6%, respectively. The level of IgG antibodies against H. contortus increased significantly after infection in each infected group. CONCLUSION: These results represent the first monitoring of the in vitro anthelmintic effects of herbal mixtures on H. contortus. The in vivo experiment indicated that the anthelmintic effect was not sufficient for the elimination of parasites, but this herbal treatment may affect the host over a longer term, reducing the parasitic infection in the host.
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spelling pubmed-67008142019-08-26 Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep Mravčáková, Dominika Váradyová, Zora Kopčáková, Anna Čobanová, Klaudia Grešáková, Ľubomíra Kišidayová, Svetlana Babják, Michal Dolinská, Michaela Urda Dvorožňáková, Emília Königová, Alžbeta Vadlejch, Jaroslav Cieslak, Adam Ślusarczyk, Sylwester Várady, Marián BMC Vet Res Research Article BACKGROUND: Parallel in vitro and in vivo experiments were designed to evaluate promising chemotherapeutic alternatives for controlling haemonchosis in ruminants. In vitro anthelmintic activities (egg hatch test – EHT; larval development test – LDT) of aqueous and methanolic herbal extracts Mix1 and Mix2 were investigated. The in vivo effects of dietary supplementation with Mix1 and Mix2 on the parasitological status, inflammatory response, antioxidant parameters and microbial community of the lambs infected experimentally with Haemonchus contortus were investigated. Lambs were divided into four groups for the in vivo study: uninfected control lambs (C), infected lambs (I), infected lambs supplemented with Mix1 (I + Mix1) and infected lambs supplemented with Mix2 (I + Mix2). The experimental period was 70 days. RESULTS: The number of eggs per gram (EPG) of feces was quantified 22, 30, 37, 44, 51, 58, 65 and 70 days post-infection, and mean abomasal worm counts were assessed 70 days post-infection. Quantitative analyses identified 57.3 and 22.2 mg/g phenolic acids, 41.5 and 29.5 mg/g flavonoids and 1.4 and 1.33 mg/g protoberberine-type alkaloids in Mix1 and Mix2, respectively. The methanolic extracts of the herbal mixtures in both in vitro tests had higher anthelmintic effects (P < 0.01) than the aqueous extracts, but the effects did not differ significantly between Mix1 and Mix2 (P > 0.05). I + Mix1 and I + Mix2 lowered mean EPGs between 44 and 70 d by 58.1 and 51.6%, respectively. The level of IgG antibodies against H. contortus increased significantly after infection in each infected group. CONCLUSION: These results represent the first monitoring of the in vitro anthelmintic effects of herbal mixtures on H. contortus. The in vivo experiment indicated that the anthelmintic effect was not sufficient for the elimination of parasites, but this herbal treatment may affect the host over a longer term, reducing the parasitic infection in the host. BioMed Central 2019-08-20 /pmc/articles/PMC6700814/ /pubmed/31429752 http://dx.doi.org/10.1186/s12917-019-2050-2 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Mravčáková, Dominika
Váradyová, Zora
Kopčáková, Anna
Čobanová, Klaudia
Grešáková, Ľubomíra
Kišidayová, Svetlana
Babják, Michal
Dolinská, Michaela Urda
Dvorožňáková, Emília
Königová, Alžbeta
Vadlejch, Jaroslav
Cieslak, Adam
Ślusarczyk, Sylwester
Várady, Marián
Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep
title Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep
title_full Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep
title_fullStr Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep
title_full_unstemmed Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep
title_short Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep
title_sort natural chemotherapeutic alternatives for controlling of haemonchosis in sheep
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700814/
https://www.ncbi.nlm.nih.gov/pubmed/31429752
http://dx.doi.org/10.1186/s12917-019-2050-2
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