Cargando…

Targeted transcript quantification in single disseminated cancer cells after whole transcriptome amplification

Gene expression analysis of rare or heterogeneous cell populations such as disseminated cancer cells (DCCs) requires a sensitive method allowing reliable analysis of single cells. Therefore, we developed and explored the feasibility of a quantitative PCR (qPCR) assay to analyze single-cell cDNA pre-...

Descripción completa

Detalles Bibliográficos
Autores principales:	Durst, Franziska C., Grujovic, Ana, Ganser, Iris, Hoffmann, Martin, Ugocsai, Peter, Klein, Christoph A., Czyż, Zbigniew T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701776/ https://www.ncbi.nlm.nih.gov/pubmed/31430289 http://dx.doi.org/10.1371/journal.pone.0216442

Ejemplares similares

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
por: Chen, Shukun, et al.
Publicado: (2018)

Reliable Single Cell Array CGH for Clinical Samples
por: Czyż, Zbigniew T., et al.
Publicado: (2014)

Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
por: Wienholz, Franziska, et al.
Publicado: (2017)

Quantification of Trace-Level DNA by Real-Time Whole Genome Amplification
por: Kang, Min-Jung, et al.
Publicado: (2011)

Single Cell Transcriptome Amplification with MALBAC
por: Chapman, Alec R., et al.
Publicado: (2015)

Quantification of a Neurological Protein in a Single Cell Without Amplification
por: Lee, Donggyu, et al.
Publicado: (2022)

Single cell on-chip whole genome amplification via micropillar arrays for reduced amplification bias
por: Tian, Harvey C., et al.
Publicado: (2018)

Evaluating whole transcriptome amplification for gene profiling experiments using RNA-Seq
por: Faherty, Sheena L, et al.
Publicado: (2015)

Effect of de novo transcriptome assembly on transcript quantification
por: Hsieh, Ping-Han, et al.
Publicado: (2019)

Decontamination of MDA Reagents for Single Cell Whole Genome Amplification
por: Woyke, Tanja, et al.
Publicado: (2011)

Kinship analysis on single cells after whole genome amplification
por: Weymaere, Jana, et al.
Publicado: (2020)

Whole Genome Amplification and De novo Assembly of Single Bacterial Cells
por: Rodrigue, Sébastien, et al.
Publicado: (2009)

A Quantitative Comparison of Single-Cell Whole Genome Amplification Methods
por: de Bourcy, Charles F. A., et al.
Publicado: (2014)

Molecular Profiling of Single Sca-1(+)/CD34(+,−) Cells—The Putative Murine Lung Stem Cells
por: Hittinger, Markus, et al.
Publicado: (2013)

Identification of small gains and losses in single cells after whole genome amplification on tiling oligo arrays
por: Geigl, Jochen B., et al.
Publicado: (2009)

Influence of primer & probe chemistry and amplification target on reverse transcription digital PCR quantification of viral RNA
por: Van Heuverswyn, Fran, et al.
Publicado: (2016)

BRIE: transcriptome-wide splicing quantification in single cells
por: Huang, Yuanhua, et al.
Publicado: (2017)

Feasibility of Whole RNA Sequencing from Single-Cell mRNA Amplification
por: Xu, Yunbo, et al.
Publicado: (2013)

Comparison of whole genome amplification techniques for human single cell exome sequencing
por: Borgström, Erik, et al.
Publicado: (2017)

A Comparison of Single Molecule and Amplification Based Sequencing of Cancer Transcriptomes
por: Sam, Lee T., et al.
Publicado: (2011)

Lactation transcriptomics in the Australian marsupial, Macropus eugenii: transcript sequencing and quantification
por: Lefèvre, Christophe M, et al.
Publicado: (2007)

Author Correction: Effect of de novo transcriptome assembly on transcript quantification
por: Hsieh, Ping-Han, et al.
Publicado: (2019)

Whole transcriptome targeted gene quantification provides new insights on pulmonary sarcomatoid carcinomas
por: Alì, Greta, et al.
Publicado: (2019)

Whole body MR and disseminated cysticercosis
por: Goenka, Ajit Harishkumar, et al.
Publicado: (2011)

Skin dissemination of myeloma
por: Męcińska-Jundziłł, Kaja, et al.
Publicado: (2017)

Primase-based whole genome amplification
por: Li, Ying, et al.
Publicado: (2008)

Special Issue on Whole Genome Amplification
por: Jäger, Richard
Publicado: (2023)

Refined Quantification of Infection Bottlenecks and Pathogen Dissemination with STAMPR
por: Hullahalli, Karthik, et al.
Publicado: (2021)

Transcriptome and proteome quantification of a tumor model provides novel insights into post‐transcriptional gene regulation
por: Jüschke, Christoph, et al.
Publicado: (2013)

Uncertainty quantification in the radiogenomics modeling of EGFR amplification in glioblastoma
por: Hu, Leland S., et al.
Publicado: (2021)

Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
por: Bäumer, Christiane, et al.
Publicado: (2018)

Massively parallel whole genome amplification for single-cell sequencing using droplet microfluidics
por: Hosokawa, Masahito, et al.
Publicado: (2017)

No significant viral transcription detected in whole breast cancer transcriptomes
por: Fimereli, Danai, et al.
Publicado: (2015)

Real Time Multiplicative Memory Amplification Mediated by Whole-Cell Scaling of Synaptic Response in Key Neurons
por: Reuveni, Iris, et al.
Publicado: (2017)

The transcriptional regulator CBX2 and ovarian function: A whole genome and whole transcriptome approach
por: Bouazzi, Leila, et al.
Publicado: (2019)

Dissecting transcriptional amplification by MYC
por: Nie, Zuqin, et al.
Publicado: (2020)

Whole-Genome Sequencing of Six Neglected Arboviruses Circulating in Africa Using Sequence-Independent Single Primer Amplification (SISPA) and MinION Nanopore Technologies
por: Schulz, Ansgar, et al.
Publicado: (2022)

Accurate quantification of DNA using on-site PCR (osPCR) by characterizing DNA amplification at single-molecule resolution
por: Ding, Ruihua, et al.
Publicado: (2023)

Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing
por: Hou, Yong, et al.
Publicado: (2015)

Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells
por: Deleye, Lieselot, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_u4a90r1sn6bmo53o1ki1aeghjn