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Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy

Nanometre-scale observation of specimens in water is indispensable in several scientific fields, such as biology, chemistry, materials science and nanotechnology. Scanning electron microscopy (SEM) obtains high-resolution images of biological samples under high vacuum conditions but requires specifi...

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Autor principal: Ogura, Toshihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701803/
https://www.ncbi.nlm.nih.gov/pubmed/31430321
http://dx.doi.org/10.1371/journal.pone.0221296
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author Ogura, Toshihiko
author_facet Ogura, Toshihiko
author_sort Ogura, Toshihiko
collection PubMed
description Nanometre-scale observation of specimens in water is indispensable in several scientific fields, such as biology, chemistry, materials science and nanotechnology. Scanning electron microscopy (SEM) obtains high-resolution images of biological samples under high vacuum conditions but requires specific sample-preparation protocols. Observations of unstained biological samples in water require more convenient and less invasive methods. Herein, we have developed a new type of impedance microscopy, namely impedance SEM (IP-SEM), which allows the imaging and sub-micrometer scale examination of various specimens in water. By varying the frequency of the input signal, the proposed system can detect the impedance properties of the sample’s composition at sub-micrometer scale resolution. Besides examining various unstained biological specimens and material samples in water. Furthermore, the proposed system can be used for diverse liquid samples across a broad range of scientific fields, such as nanoparticles, nanotubes and organic and catalytic materials.
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spelling pubmed-67018032019-09-04 Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy Ogura, Toshihiko PLoS One Research Article Nanometre-scale observation of specimens in water is indispensable in several scientific fields, such as biology, chemistry, materials science and nanotechnology. Scanning electron microscopy (SEM) obtains high-resolution images of biological samples under high vacuum conditions but requires specific sample-preparation protocols. Observations of unstained biological samples in water require more convenient and less invasive methods. Herein, we have developed a new type of impedance microscopy, namely impedance SEM (IP-SEM), which allows the imaging and sub-micrometer scale examination of various specimens in water. By varying the frequency of the input signal, the proposed system can detect the impedance properties of the sample’s composition at sub-micrometer scale resolution. Besides examining various unstained biological specimens and material samples in water. Furthermore, the proposed system can be used for diverse liquid samples across a broad range of scientific fields, such as nanoparticles, nanotubes and organic and catalytic materials. Public Library of Science 2019-08-20 /pmc/articles/PMC6701803/ /pubmed/31430321 http://dx.doi.org/10.1371/journal.pone.0221296 Text en © 2019 Toshihiko Ogura http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ogura, Toshihiko
Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
title Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
title_full Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
title_fullStr Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
title_full_unstemmed Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
title_short Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
title_sort direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701803/
https://www.ncbi.nlm.nih.gov/pubmed/31430321
http://dx.doi.org/10.1371/journal.pone.0221296
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