Cargando…
Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
Nanometre-scale observation of specimens in water is indispensable in several scientific fields, such as biology, chemistry, materials science and nanotechnology. Scanning electron microscopy (SEM) obtains high-resolution images of biological samples under high vacuum conditions but requires specifi...
Autor principal: | |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701803/ https://www.ncbi.nlm.nih.gov/pubmed/31430321 http://dx.doi.org/10.1371/journal.pone.0221296 |
_version_ | 1783445115333771264 |
---|---|
author | Ogura, Toshihiko |
author_facet | Ogura, Toshihiko |
author_sort | Ogura, Toshihiko |
collection | PubMed |
description | Nanometre-scale observation of specimens in water is indispensable in several scientific fields, such as biology, chemistry, materials science and nanotechnology. Scanning electron microscopy (SEM) obtains high-resolution images of biological samples under high vacuum conditions but requires specific sample-preparation protocols. Observations of unstained biological samples in water require more convenient and less invasive methods. Herein, we have developed a new type of impedance microscopy, namely impedance SEM (IP-SEM), which allows the imaging and sub-micrometer scale examination of various specimens in water. By varying the frequency of the input signal, the proposed system can detect the impedance properties of the sample’s composition at sub-micrometer scale resolution. Besides examining various unstained biological specimens and material samples in water. Furthermore, the proposed system can be used for diverse liquid samples across a broad range of scientific fields, such as nanoparticles, nanotubes and organic and catalytic materials. |
format | Online Article Text |
id | pubmed-6701803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67018032019-09-04 Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy Ogura, Toshihiko PLoS One Research Article Nanometre-scale observation of specimens in water is indispensable in several scientific fields, such as biology, chemistry, materials science and nanotechnology. Scanning electron microscopy (SEM) obtains high-resolution images of biological samples under high vacuum conditions but requires specific sample-preparation protocols. Observations of unstained biological samples in water require more convenient and less invasive methods. Herein, we have developed a new type of impedance microscopy, namely impedance SEM (IP-SEM), which allows the imaging and sub-micrometer scale examination of various specimens in water. By varying the frequency of the input signal, the proposed system can detect the impedance properties of the sample’s composition at sub-micrometer scale resolution. Besides examining various unstained biological specimens and material samples in water. Furthermore, the proposed system can be used for diverse liquid samples across a broad range of scientific fields, such as nanoparticles, nanotubes and organic and catalytic materials. Public Library of Science 2019-08-20 /pmc/articles/PMC6701803/ /pubmed/31430321 http://dx.doi.org/10.1371/journal.pone.0221296 Text en © 2019 Toshihiko Ogura http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ogura, Toshihiko Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy |
title | Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy |
title_full | Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy |
title_fullStr | Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy |
title_full_unstemmed | Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy |
title_short | Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy |
title_sort | direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701803/ https://www.ncbi.nlm.nih.gov/pubmed/31430321 http://dx.doi.org/10.1371/journal.pone.0221296 |
work_keys_str_mv | AT oguratoshihiko directobservationofunstainedbiologicalsamplesinwaterusingnewlydevelopedimpedancescanningelectronmicroscopy |