Cargando…
Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil
Microbial activity is the main route for cycling mangrove nutrients. In general, microorganisms have abilities to degrade lignocellulosic compounds. Among the biotechnological potential of the microbiota from mangroves, it is noteworthy about endophytic fungi, which can be considered as effective so...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702500/ https://www.ncbi.nlm.nih.gov/pubmed/31428885 http://dx.doi.org/10.1186/s13568-019-0850-1 |
_version_ | 1783445238105243648 |
---|---|
author | Martinho, Vivian dos Santos Lima, Lidiane Maria Barros, Caroline Almeida Ferrari, Vitor Baptista Passarini, Michel Rodrigo Zambrano Santos, Leonardo André de Souza Sebastianes, Fernanda Luisa Lacava, Paulo Teixeira de Vasconcellos, Suzan Pantaroto |
author_facet | Martinho, Vivian dos Santos Lima, Lidiane Maria Barros, Caroline Almeida Ferrari, Vitor Baptista Passarini, Michel Rodrigo Zambrano Santos, Leonardo André de Souza Sebastianes, Fernanda Luisa Lacava, Paulo Teixeira de Vasconcellos, Suzan Pantaroto |
author_sort | Martinho, Vivian |
collection | PubMed |
description | Microbial activity is the main route for cycling mangrove nutrients. In general, microorganisms have abilities to degrade lignocellulosic compounds. Among the biotechnological potential of the microbiota from mangroves, it is noteworthy about endophytic fungi, which can be considered as effective sources of different bioactive compounds. In this sense, thirty (30) endophytic fungi were isolated from mangrove forest sampling Cananeia, SP, Brazil. These microorganisms were analyzed about their enzymatic activities including: lignin peroxidase EC 1.11.1.14, manganese peroxidase EC 1.11.1.13 and laccase EC 1.10.3.2, as well endo-cellulase EC 3.2.1.4 and endo-xylanase EC 3.2.1.8. Besides that, production of bioactive secondary metabolites like biosurfactant and/or bioemulsifier was also investigated. As results, nineteen (19) isolates were selected about their ligninolytic abilities, nine (9) of them about cellulase activity and thirteen (13) showed xylanase abilities. The fungal isolate named as 3(3), characterized as Fusarium sambucinum, showed a prominent lignin peroxidase (42.4 U L(−1)) and manganese peroxidase (23.6 U L(−1)) activities. The isolate 63.1, also related to Fusarium sp. genera, was selected about its laccase activity (41.5 U L(−1)). From all the investigated fungi, the isolate 47(4) Trichoderma camerunense was selected about its cellulolytic and xylanolytic activities, showing 45.23 and 26.09 U mL(−1), respectively. The same fungi also showed biosurfactant ability demonstrated by superficial tension decreasing to 38 mN/m. In addition, fifteen (15) fungi exhibited bioemulsifier activity, with E(24) values up to 62.8%. |
format | Online Article Text |
id | pubmed-6702500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-67025002019-09-02 Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil Martinho, Vivian dos Santos Lima, Lidiane Maria Barros, Caroline Almeida Ferrari, Vitor Baptista Passarini, Michel Rodrigo Zambrano Santos, Leonardo André de Souza Sebastianes, Fernanda Luisa Lacava, Paulo Teixeira de Vasconcellos, Suzan Pantaroto AMB Express Original Article Microbial activity is the main route for cycling mangrove nutrients. In general, microorganisms have abilities to degrade lignocellulosic compounds. Among the biotechnological potential of the microbiota from mangroves, it is noteworthy about endophytic fungi, which can be considered as effective sources of different bioactive compounds. In this sense, thirty (30) endophytic fungi were isolated from mangrove forest sampling Cananeia, SP, Brazil. These microorganisms were analyzed about their enzymatic activities including: lignin peroxidase EC 1.11.1.14, manganese peroxidase EC 1.11.1.13 and laccase EC 1.10.3.2, as well endo-cellulase EC 3.2.1.4 and endo-xylanase EC 3.2.1.8. Besides that, production of bioactive secondary metabolites like biosurfactant and/or bioemulsifier was also investigated. As results, nineteen (19) isolates were selected about their ligninolytic abilities, nine (9) of them about cellulase activity and thirteen (13) showed xylanase abilities. The fungal isolate named as 3(3), characterized as Fusarium sambucinum, showed a prominent lignin peroxidase (42.4 U L(−1)) and manganese peroxidase (23.6 U L(−1)) activities. The isolate 63.1, also related to Fusarium sp. genera, was selected about its laccase activity (41.5 U L(−1)). From all the investigated fungi, the isolate 47(4) Trichoderma camerunense was selected about its cellulolytic and xylanolytic activities, showing 45.23 and 26.09 U mL(−1), respectively. The same fungi also showed biosurfactant ability demonstrated by superficial tension decreasing to 38 mN/m. In addition, fifteen (15) fungi exhibited bioemulsifier activity, with E(24) values up to 62.8%. Springer Berlin Heidelberg 2019-08-19 /pmc/articles/PMC6702500/ /pubmed/31428885 http://dx.doi.org/10.1186/s13568-019-0850-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Martinho, Vivian dos Santos Lima, Lidiane Maria Barros, Caroline Almeida Ferrari, Vitor Baptista Passarini, Michel Rodrigo Zambrano Santos, Leonardo André de Souza Sebastianes, Fernanda Luisa Lacava, Paulo Teixeira de Vasconcellos, Suzan Pantaroto Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil |
title | Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil |
title_full | Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil |
title_fullStr | Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil |
title_full_unstemmed | Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil |
title_short | Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil |
title_sort | enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in southeastern brazil |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702500/ https://www.ncbi.nlm.nih.gov/pubmed/31428885 http://dx.doi.org/10.1186/s13568-019-0850-1 |
work_keys_str_mv | AT martinhovivian enzymaticpotentialandbiosurfactantproductionbyendophyticfungifrommangroveforestinsoutheasternbrazil AT dossantoslimalidianemaria enzymaticpotentialandbiosurfactantproductionbyendophyticfungifrommangroveforestinsoutheasternbrazil AT barroscarolinealmeida enzymaticpotentialandbiosurfactantproductionbyendophyticfungifrommangroveforestinsoutheasternbrazil AT ferrarivitorbaptista enzymaticpotentialandbiosurfactantproductionbyendophyticfungifrommangroveforestinsoutheasternbrazil AT passarinimichelrodrigozambrano enzymaticpotentialandbiosurfactantproductionbyendophyticfungifrommangroveforestinsoutheasternbrazil AT santosleonardoandre enzymaticpotentialandbiosurfactantproductionbyendophyticfungifrommangroveforestinsoutheasternbrazil AT desouzasebastianesfernandaluisa enzymaticpotentialandbiosurfactantproductionbyendophyticfungifrommangroveforestinsoutheasternbrazil AT lacavapauloteixeira enzymaticpotentialandbiosurfactantproductionbyendophyticfungifrommangroveforestinsoutheasternbrazil AT devasconcellossuzanpantaroto enzymaticpotentialandbiosurfactantproductionbyendophyticfungifrommangroveforestinsoutheasternbrazil |