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Phenotypic detection of methicillin-resistant Staphylococcus aureus in village chickens from poultry markets in Maiduguri, Nigeria

OBJECTIVE: The study was carried out to detect the carriage of methicillin-resistant Staphylococcus aureus (MRSA) and their antimicrobial susceptibilities in village chickens sold at Maiduguri poultry markets using phenotypic characterization. MATERIALS AND METHODS: This was a cross-sectional study...

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Autores principales: Kwoji, Iliya Dauda, Jauro, Solomon, Musa, Jasini Athanda, Lekko, Yusuf Madaki, Salihu, Sabo Isa, Danchuwa, Hassan Abdullahi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702879/
https://www.ncbi.nlm.nih.gov/pubmed/31453186
http://dx.doi.org/10.5455/javar.2019.f327
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author Kwoji, Iliya Dauda
Jauro, Solomon
Musa, Jasini Athanda
Lekko, Yusuf Madaki
Salihu, Sabo Isa
Danchuwa, Hassan Abdullahi
author_facet Kwoji, Iliya Dauda
Jauro, Solomon
Musa, Jasini Athanda
Lekko, Yusuf Madaki
Salihu, Sabo Isa
Danchuwa, Hassan Abdullahi
author_sort Kwoji, Iliya Dauda
collection PubMed
description OBJECTIVE: The study was carried out to detect the carriage of methicillin-resistant Staphylococcus aureus (MRSA) and their antimicrobial susceptibilities in village chickens sold at Maiduguri poultry markets using phenotypic characterization. MATERIALS AND METHODS: This was a cross-sectional study where 120 samples comprised 50% each of Nasal and cloacal swabs, were, respectively, collected from live village chickens sold at Maiduguri poultry markets and examined for the presence MRSA based on bacterial culture, biochemical characteristics, growth on oxacillin resistant screening agar base, and antimicrobial susceptibility tests. RESULTS: The study revealed an overall occurrence of 38.33% S. aureus and 32.60% MRSA, respectively. Antimicrobial susceptibility test was carried out on MRSA positive isolates against seven antimicrobials. All the isolates (100%) exhibited resistance against cefoxitin, whereas the least antimicrobial resistance was recorded against erythromycin and streptomycin each with 26.6%, respectively. In the same way, the highest antibiotic susceptibility in this study was observed against erythromycin (60%) and least susceptibility was against vancomycin and streptomycin with 20% each. A varying intermediate antibiotic susceptibility ranging from 13.33% to 53.33% was observed. Multiple-drug resistance patterns were exhibited by MRSA isolates from this study with 73.3% of the isolates exhibiting resistance to two or more antibiotics. CONCLUSION: This study has shown the carriage of MRSA by village chickens which calls for serious public health concern and concludes that these birds might have acquired these pathogens from the environment or infected humans since they normally receive no less medical attention.
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spelling pubmed-67028792019-08-26 Phenotypic detection of methicillin-resistant Staphylococcus aureus in village chickens from poultry markets in Maiduguri, Nigeria Kwoji, Iliya Dauda Jauro, Solomon Musa, Jasini Athanda Lekko, Yusuf Madaki Salihu, Sabo Isa Danchuwa, Hassan Abdullahi J Adv Vet Anim Res Short Communication OBJECTIVE: The study was carried out to detect the carriage of methicillin-resistant Staphylococcus aureus (MRSA) and their antimicrobial susceptibilities in village chickens sold at Maiduguri poultry markets using phenotypic characterization. MATERIALS AND METHODS: This was a cross-sectional study where 120 samples comprised 50% each of Nasal and cloacal swabs, were, respectively, collected from live village chickens sold at Maiduguri poultry markets and examined for the presence MRSA based on bacterial culture, biochemical characteristics, growth on oxacillin resistant screening agar base, and antimicrobial susceptibility tests. RESULTS: The study revealed an overall occurrence of 38.33% S. aureus and 32.60% MRSA, respectively. Antimicrobial susceptibility test was carried out on MRSA positive isolates against seven antimicrobials. All the isolates (100%) exhibited resistance against cefoxitin, whereas the least antimicrobial resistance was recorded against erythromycin and streptomycin each with 26.6%, respectively. In the same way, the highest antibiotic susceptibility in this study was observed against erythromycin (60%) and least susceptibility was against vancomycin and streptomycin with 20% each. A varying intermediate antibiotic susceptibility ranging from 13.33% to 53.33% was observed. Multiple-drug resistance patterns were exhibited by MRSA isolates from this study with 73.3% of the isolates exhibiting resistance to two or more antibiotics. CONCLUSION: This study has shown the carriage of MRSA by village chickens which calls for serious public health concern and concludes that these birds might have acquired these pathogens from the environment or infected humans since they normally receive no less medical attention. A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET) 2019-03-23 /pmc/articles/PMC6702879/ /pubmed/31453186 http://dx.doi.org/10.5455/javar.2019.f327 Text en Copyright: © Journal of Advanced Veterinary and Animal Research http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Kwoji, Iliya Dauda
Jauro, Solomon
Musa, Jasini Athanda
Lekko, Yusuf Madaki
Salihu, Sabo Isa
Danchuwa, Hassan Abdullahi
Phenotypic detection of methicillin-resistant Staphylococcus aureus in village chickens from poultry markets in Maiduguri, Nigeria
title Phenotypic detection of methicillin-resistant Staphylococcus aureus in village chickens from poultry markets in Maiduguri, Nigeria
title_full Phenotypic detection of methicillin-resistant Staphylococcus aureus in village chickens from poultry markets in Maiduguri, Nigeria
title_fullStr Phenotypic detection of methicillin-resistant Staphylococcus aureus in village chickens from poultry markets in Maiduguri, Nigeria
title_full_unstemmed Phenotypic detection of methicillin-resistant Staphylococcus aureus in village chickens from poultry markets in Maiduguri, Nigeria
title_short Phenotypic detection of methicillin-resistant Staphylococcus aureus in village chickens from poultry markets in Maiduguri, Nigeria
title_sort phenotypic detection of methicillin-resistant staphylococcus aureus in village chickens from poultry markets in maiduguri, nigeria
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702879/
https://www.ncbi.nlm.nih.gov/pubmed/31453186
http://dx.doi.org/10.5455/javar.2019.f327
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