Cargando…
Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal
Constantly surrounded by kin or alien organisms in nature, eukaryotes and prokaryotes developed various communication systems to coordinate adaptive multi-entity behavior. In complex and overcrowded environments, they require to discriminate relevant signals in a myriad of pheromones to execute appr...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6703854/ https://www.ncbi.nlm.nih.gov/pubmed/31433299 http://dx.doi.org/10.7554/eLife.47139 |
_version_ | 1783445407303467008 |
---|---|
author | Mignolet, Johann Cerckel, Guillaume Damoczi, Julien Ledesma-Garcia, Laura Sass, Andrea Coenye, Tom Nessler, Sylvie Hols, Pascal |
author_facet | Mignolet, Johann Cerckel, Guillaume Damoczi, Julien Ledesma-Garcia, Laura Sass, Andrea Coenye, Tom Nessler, Sylvie Hols, Pascal |
author_sort | Mignolet, Johann |
collection | PubMed |
description | Constantly surrounded by kin or alien organisms in nature, eukaryotes and prokaryotes developed various communication systems to coordinate adaptive multi-entity behavior. In complex and overcrowded environments, they require to discriminate relevant signals in a myriad of pheromones to execute appropriate responses. In the human gut commensal Streptococcus salivarius, the cytoplasmic Rgg/RNPP regulator ComR couples competence to bacteriocin-mediated predation. Here, we describe a paralogous sensor duo, ScuR and SarF, which circumvents ComR in order to disconnect these two physiological processes. We highlighted the recurring role of Rgg/RNPP in the production of antimicrobials and designed a robust genetic screen to unveil potent/optimized peptide pheromones. Further mutational and biochemical analyses dissected the modifiable selectivity toward their pheromone and operating sequences at the subtle molecular level. Additionally, our results highlight how we might mobilize antimicrobial molecules while silencing competence in endogenous populations of human microflora and temper gut disorders provoked by bacterial pathogens. |
format | Online Article Text |
id | pubmed-6703854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-67038542019-08-22 Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal Mignolet, Johann Cerckel, Guillaume Damoczi, Julien Ledesma-Garcia, Laura Sass, Andrea Coenye, Tom Nessler, Sylvie Hols, Pascal eLife Biochemistry and Chemical Biology Constantly surrounded by kin or alien organisms in nature, eukaryotes and prokaryotes developed various communication systems to coordinate adaptive multi-entity behavior. In complex and overcrowded environments, they require to discriminate relevant signals in a myriad of pheromones to execute appropriate responses. In the human gut commensal Streptococcus salivarius, the cytoplasmic Rgg/RNPP regulator ComR couples competence to bacteriocin-mediated predation. Here, we describe a paralogous sensor duo, ScuR and SarF, which circumvents ComR in order to disconnect these two physiological processes. We highlighted the recurring role of Rgg/RNPP in the production of antimicrobials and designed a robust genetic screen to unveil potent/optimized peptide pheromones. Further mutational and biochemical analyses dissected the modifiable selectivity toward their pheromone and operating sequences at the subtle molecular level. Additionally, our results highlight how we might mobilize antimicrobial molecules while silencing competence in endogenous populations of human microflora and temper gut disorders provoked by bacterial pathogens. eLife Sciences Publications, Ltd 2019-08-21 /pmc/articles/PMC6703854/ /pubmed/31433299 http://dx.doi.org/10.7554/eLife.47139 Text en © 2019, Mignolet et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Mignolet, Johann Cerckel, Guillaume Damoczi, Julien Ledesma-Garcia, Laura Sass, Andrea Coenye, Tom Nessler, Sylvie Hols, Pascal Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal |
title | Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal |
title_full | Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal |
title_fullStr | Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal |
title_full_unstemmed | Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal |
title_short | Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal |
title_sort | subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6703854/ https://www.ncbi.nlm.nih.gov/pubmed/31433299 http://dx.doi.org/10.7554/eLife.47139 |
work_keys_str_mv | AT mignoletjohann subtleselectivityinapheromonesensortriumviratedesynchronizescompetenceandpredationinahumangutcommensal AT cerckelguillaume subtleselectivityinapheromonesensortriumviratedesynchronizescompetenceandpredationinahumangutcommensal AT damoczijulien subtleselectivityinapheromonesensortriumviratedesynchronizescompetenceandpredationinahumangutcommensal AT ledesmagarcialaura subtleselectivityinapheromonesensortriumviratedesynchronizescompetenceandpredationinahumangutcommensal AT sassandrea subtleselectivityinapheromonesensortriumviratedesynchronizescompetenceandpredationinahumangutcommensal AT coenyetom subtleselectivityinapheromonesensortriumviratedesynchronizescompetenceandpredationinahumangutcommensal AT nesslersylvie subtleselectivityinapheromonesensortriumviratedesynchronizescompetenceandpredationinahumangutcommensal AT holspascal subtleselectivityinapheromonesensortriumviratedesynchronizescompetenceandpredationinahumangutcommensal |