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Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal

Constantly surrounded by kin or alien organisms in nature, eukaryotes and prokaryotes developed various communication systems to coordinate adaptive multi-entity behavior. In complex and overcrowded environments, they require to discriminate relevant signals in a myriad of pheromones to execute appr...

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Autores principales: Mignolet, Johann, Cerckel, Guillaume, Damoczi, Julien, Ledesma-Garcia, Laura, Sass, Andrea, Coenye, Tom, Nessler, Sylvie, Hols, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6703854/
https://www.ncbi.nlm.nih.gov/pubmed/31433299
http://dx.doi.org/10.7554/eLife.47139
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author Mignolet, Johann
Cerckel, Guillaume
Damoczi, Julien
Ledesma-Garcia, Laura
Sass, Andrea
Coenye, Tom
Nessler, Sylvie
Hols, Pascal
author_facet Mignolet, Johann
Cerckel, Guillaume
Damoczi, Julien
Ledesma-Garcia, Laura
Sass, Andrea
Coenye, Tom
Nessler, Sylvie
Hols, Pascal
author_sort Mignolet, Johann
collection PubMed
description Constantly surrounded by kin or alien organisms in nature, eukaryotes and prokaryotes developed various communication systems to coordinate adaptive multi-entity behavior. In complex and overcrowded environments, they require to discriminate relevant signals in a myriad of pheromones to execute appropriate responses. In the human gut commensal Streptococcus salivarius, the cytoplasmic Rgg/RNPP regulator ComR couples competence to bacteriocin-mediated predation. Here, we describe a paralogous sensor duo, ScuR and SarF, which circumvents ComR in order to disconnect these two physiological processes. We highlighted the recurring role of Rgg/RNPP in the production of antimicrobials and designed a robust genetic screen to unveil potent/optimized peptide pheromones. Further mutational and biochemical analyses dissected the modifiable selectivity toward their pheromone and operating sequences at the subtle molecular level. Additionally, our results highlight how we might mobilize antimicrobial molecules while silencing competence in endogenous populations of human microflora and temper gut disorders provoked by bacterial pathogens.
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spelling pubmed-67038542019-08-22 Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal Mignolet, Johann Cerckel, Guillaume Damoczi, Julien Ledesma-Garcia, Laura Sass, Andrea Coenye, Tom Nessler, Sylvie Hols, Pascal eLife Biochemistry and Chemical Biology Constantly surrounded by kin or alien organisms in nature, eukaryotes and prokaryotes developed various communication systems to coordinate adaptive multi-entity behavior. In complex and overcrowded environments, they require to discriminate relevant signals in a myriad of pheromones to execute appropriate responses. In the human gut commensal Streptococcus salivarius, the cytoplasmic Rgg/RNPP regulator ComR couples competence to bacteriocin-mediated predation. Here, we describe a paralogous sensor duo, ScuR and SarF, which circumvents ComR in order to disconnect these two physiological processes. We highlighted the recurring role of Rgg/RNPP in the production of antimicrobials and designed a robust genetic screen to unveil potent/optimized peptide pheromones. Further mutational and biochemical analyses dissected the modifiable selectivity toward their pheromone and operating sequences at the subtle molecular level. Additionally, our results highlight how we might mobilize antimicrobial molecules while silencing competence in endogenous populations of human microflora and temper gut disorders provoked by bacterial pathogens. eLife Sciences Publications, Ltd 2019-08-21 /pmc/articles/PMC6703854/ /pubmed/31433299 http://dx.doi.org/10.7554/eLife.47139 Text en © 2019, Mignolet et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Mignolet, Johann
Cerckel, Guillaume
Damoczi, Julien
Ledesma-Garcia, Laura
Sass, Andrea
Coenye, Tom
Nessler, Sylvie
Hols, Pascal
Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal
title Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal
title_full Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal
title_fullStr Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal
title_full_unstemmed Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal
title_short Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal
title_sort subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6703854/
https://www.ncbi.nlm.nih.gov/pubmed/31433299
http://dx.doi.org/10.7554/eLife.47139
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