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Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system
The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous studies have shown that shootin1 couples actin filament retrograde flow with...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704158/ https://www.ncbi.nlm.nih.gov/pubmed/31434971 http://dx.doi.org/10.1038/s41598-019-48585-4 |
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author | Urasaki, Akihiro Morishita, Seiya Naka, Kosuke Uozumi, Minato Abe, Kouki Huang, Liguo Watase, Emiko Nakagawa, Osamu Kawakami, Koichi Matsui, Takaaki Bessho, Yasumasa Inagaki, Naoyuki |
author_facet | Urasaki, Akihiro Morishita, Seiya Naka, Kosuke Uozumi, Minato Abe, Kouki Huang, Liguo Watase, Emiko Nakagawa, Osamu Kawakami, Koichi Matsui, Takaaki Bessho, Yasumasa Inagaki, Naoyuki |
author_sort | Urasaki, Akihiro |
collection | PubMed |
description | The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous studies have shown that shootin1 couples actin filament retrograde flow with extracellular adhesive substrates at the leading edge of axonal growth cones, thereby producing mechanical force for the migration and guidance of axonal growth cones. However, the functions of shootin in peripheral cells remain unknown. Here we identified two novel shootin family members, shootin2 and shootin3. In zebrafish, shootin1 and shootin3 are expressed in the posterior lateral line primordium (PLLP) and neuromasts during embryonic development. A shootin1 mutant displayed a reduced speed of PLLP migration, while shootin1;shootin3 double mutation inhibited cell proliferation in the PLLP. Furthermore, our results suggest that shootin1 and shootin3 positively regulate the number of neuromasts and the number of cells in deposited neuromasts. Our study demonstrates that shootins mediate collective cell migration of the posterior lateral line primordium and formation of neuromasts in zebrafish. |
format | Online Article Text |
id | pubmed-6704158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67041582019-08-23 Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system Urasaki, Akihiro Morishita, Seiya Naka, Kosuke Uozumi, Minato Abe, Kouki Huang, Liguo Watase, Emiko Nakagawa, Osamu Kawakami, Koichi Matsui, Takaaki Bessho, Yasumasa Inagaki, Naoyuki Sci Rep Article The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous studies have shown that shootin1 couples actin filament retrograde flow with extracellular adhesive substrates at the leading edge of axonal growth cones, thereby producing mechanical force for the migration and guidance of axonal growth cones. However, the functions of shootin in peripheral cells remain unknown. Here we identified two novel shootin family members, shootin2 and shootin3. In zebrafish, shootin1 and shootin3 are expressed in the posterior lateral line primordium (PLLP) and neuromasts during embryonic development. A shootin1 mutant displayed a reduced speed of PLLP migration, while shootin1;shootin3 double mutation inhibited cell proliferation in the PLLP. Furthermore, our results suggest that shootin1 and shootin3 positively regulate the number of neuromasts and the number of cells in deposited neuromasts. Our study demonstrates that shootins mediate collective cell migration of the posterior lateral line primordium and formation of neuromasts in zebrafish. Nature Publishing Group UK 2019-08-21 /pmc/articles/PMC6704158/ /pubmed/31434971 http://dx.doi.org/10.1038/s41598-019-48585-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Urasaki, Akihiro Morishita, Seiya Naka, Kosuke Uozumi, Minato Abe, Kouki Huang, Liguo Watase, Emiko Nakagawa, Osamu Kawakami, Koichi Matsui, Takaaki Bessho, Yasumasa Inagaki, Naoyuki Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system |
title | Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system |
title_full | Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system |
title_fullStr | Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system |
title_full_unstemmed | Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system |
title_short | Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system |
title_sort | shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704158/ https://www.ncbi.nlm.nih.gov/pubmed/31434971 http://dx.doi.org/10.1038/s41598-019-48585-4 |
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