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Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system

The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous studies have shown that shootin1 couples actin filament retrograde flow with...

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Autores principales: Urasaki, Akihiro, Morishita, Seiya, Naka, Kosuke, Uozumi, Minato, Abe, Kouki, Huang, Liguo, Watase, Emiko, Nakagawa, Osamu, Kawakami, Koichi, Matsui, Takaaki, Bessho, Yasumasa, Inagaki, Naoyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704158/
https://www.ncbi.nlm.nih.gov/pubmed/31434971
http://dx.doi.org/10.1038/s41598-019-48585-4
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author Urasaki, Akihiro
Morishita, Seiya
Naka, Kosuke
Uozumi, Minato
Abe, Kouki
Huang, Liguo
Watase, Emiko
Nakagawa, Osamu
Kawakami, Koichi
Matsui, Takaaki
Bessho, Yasumasa
Inagaki, Naoyuki
author_facet Urasaki, Akihiro
Morishita, Seiya
Naka, Kosuke
Uozumi, Minato
Abe, Kouki
Huang, Liguo
Watase, Emiko
Nakagawa, Osamu
Kawakami, Koichi
Matsui, Takaaki
Bessho, Yasumasa
Inagaki, Naoyuki
author_sort Urasaki, Akihiro
collection PubMed
description The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous studies have shown that shootin1 couples actin filament retrograde flow with extracellular adhesive substrates at the leading edge of axonal growth cones, thereby producing mechanical force for the migration and guidance of axonal growth cones. However, the functions of shootin in peripheral cells remain unknown. Here we identified two novel shootin family members, shootin2 and shootin3. In zebrafish, shootin1 and shootin3 are expressed in the posterior lateral line primordium (PLLP) and neuromasts during embryonic development. A shootin1 mutant displayed a reduced speed of PLLP migration, while shootin1;shootin3 double mutation inhibited cell proliferation in the PLLP. Furthermore, our results suggest that shootin1 and shootin3 positively regulate the number of neuromasts and the number of cells in deposited neuromasts. Our study demonstrates that shootins mediate collective cell migration of the posterior lateral line primordium and formation of neuromasts in zebrafish.
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spelling pubmed-67041582019-08-23 Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system Urasaki, Akihiro Morishita, Seiya Naka, Kosuke Uozumi, Minato Abe, Kouki Huang, Liguo Watase, Emiko Nakagawa, Osamu Kawakami, Koichi Matsui, Takaaki Bessho, Yasumasa Inagaki, Naoyuki Sci Rep Article The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous studies have shown that shootin1 couples actin filament retrograde flow with extracellular adhesive substrates at the leading edge of axonal growth cones, thereby producing mechanical force for the migration and guidance of axonal growth cones. However, the functions of shootin in peripheral cells remain unknown. Here we identified two novel shootin family members, shootin2 and shootin3. In zebrafish, shootin1 and shootin3 are expressed in the posterior lateral line primordium (PLLP) and neuromasts during embryonic development. A shootin1 mutant displayed a reduced speed of PLLP migration, while shootin1;shootin3 double mutation inhibited cell proliferation in the PLLP. Furthermore, our results suggest that shootin1 and shootin3 positively regulate the number of neuromasts and the number of cells in deposited neuromasts. Our study demonstrates that shootins mediate collective cell migration of the posterior lateral line primordium and formation of neuromasts in zebrafish. Nature Publishing Group UK 2019-08-21 /pmc/articles/PMC6704158/ /pubmed/31434971 http://dx.doi.org/10.1038/s41598-019-48585-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Urasaki, Akihiro
Morishita, Seiya
Naka, Kosuke
Uozumi, Minato
Abe, Kouki
Huang, Liguo
Watase, Emiko
Nakagawa, Osamu
Kawakami, Koichi
Matsui, Takaaki
Bessho, Yasumasa
Inagaki, Naoyuki
Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system
title Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system
title_full Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system
title_fullStr Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system
title_full_unstemmed Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system
title_short Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system
title_sort shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704158/
https://www.ncbi.nlm.nih.gov/pubmed/31434971
http://dx.doi.org/10.1038/s41598-019-48585-4
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