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Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non-small cell lung cancer
Leucine rich repeat LGI family member 3 (LGI3) is a member of the LGI protein family. Our previous studies reported that LGI3 was expressed in adipose tissues, brain and skin, where it served roles as a multifunctional cytokine and pro-inflammatory adipokine. It was hypothesized that LGI3 may be inv...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704323/ https://www.ncbi.nlm.nih.gov/pubmed/31452819 http://dx.doi.org/10.3892/ol.2019.10648 |
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author | Kim, Dong-Seok Kwon, Nyoun Soo Yun, Hye-Young |
author_facet | Kim, Dong-Seok Kwon, Nyoun Soo Yun, Hye-Young |
author_sort | Kim, Dong-Seok |
collection | PubMed |
description | Leucine rich repeat LGI family member 3 (LGI3) is a member of the LGI protein family. Our previous studies reported that LGI3 was expressed in adipose tissues, brain and skin, where it served roles as a multifunctional cytokine and pro-inflammatory adipokine. It was hypothesized that LGI3 may be involved in cytokine networks in cancer. The present study aimed to analyze differentially expressed genes in non-small cell lung cancer (NSCLC) tissues and NSCLC cohort data, to evaluate the prognostic role of LGI3. Expression microarray and NSCLC cohort data were statistically analyzed by bioinformatic methods, and protein-protein interactions, functional enrichment and pathway, gene coexpression network (GCN) and prognostic association analyses were performed. The results demonstrated that the expression levels of LGI3 and its receptor a disintegrin and metalloproteinase domain-containing protein 22 were significantly decreased in NSCLC tissues. A total of two upregulated genes and 11 downregulated genes in NSCLC tissues were identified as LGI3-regulated genes. Protein-protein interaction network analysis demonstrated that all LGI3-regulated genes that were altered in NSCLC were involved in a protein-protein interaction network cluster. Functional enrichment, Kyoto Encyclopedia of Genes and Genomes pathway and GCN analyses demonstrated the association of these genes with the immune and inflammatory responses, angiogenesis, the tumor necrosis factor pathway, and chemokine and peroxisome proliferator-activated receptor signaling pathways. Analysis of NSCLC cohorts revealed that low expression levels of LGI3 was significantly associated with poor prognosis of NSCLC. Analysis of the somatic mutations of the LGI3 gene in NSCLC revealed that the amino acid residues altered in NSCLC included two single nucleotide polymorphism sites and three phylogenetically coevolved amino acid residues. Taken together, these results suggest that LGI3 may be a potential prognostic marker of NSCLC. |
format | Online Article Text |
id | pubmed-6704323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-67043232019-08-26 Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non-small cell lung cancer Kim, Dong-Seok Kwon, Nyoun Soo Yun, Hye-Young Oncol Lett Articles Leucine rich repeat LGI family member 3 (LGI3) is a member of the LGI protein family. Our previous studies reported that LGI3 was expressed in adipose tissues, brain and skin, where it served roles as a multifunctional cytokine and pro-inflammatory adipokine. It was hypothesized that LGI3 may be involved in cytokine networks in cancer. The present study aimed to analyze differentially expressed genes in non-small cell lung cancer (NSCLC) tissues and NSCLC cohort data, to evaluate the prognostic role of LGI3. Expression microarray and NSCLC cohort data were statistically analyzed by bioinformatic methods, and protein-protein interactions, functional enrichment and pathway, gene coexpression network (GCN) and prognostic association analyses were performed. The results demonstrated that the expression levels of LGI3 and its receptor a disintegrin and metalloproteinase domain-containing protein 22 were significantly decreased in NSCLC tissues. A total of two upregulated genes and 11 downregulated genes in NSCLC tissues were identified as LGI3-regulated genes. Protein-protein interaction network analysis demonstrated that all LGI3-regulated genes that were altered in NSCLC were involved in a protein-protein interaction network cluster. Functional enrichment, Kyoto Encyclopedia of Genes and Genomes pathway and GCN analyses demonstrated the association of these genes with the immune and inflammatory responses, angiogenesis, the tumor necrosis factor pathway, and chemokine and peroxisome proliferator-activated receptor signaling pathways. Analysis of NSCLC cohorts revealed that low expression levels of LGI3 was significantly associated with poor prognosis of NSCLC. Analysis of the somatic mutations of the LGI3 gene in NSCLC revealed that the amino acid residues altered in NSCLC included two single nucleotide polymorphism sites and three phylogenetically coevolved amino acid residues. Taken together, these results suggest that LGI3 may be a potential prognostic marker of NSCLC. D.A. Spandidos 2019-09 2019-07-22 /pmc/articles/PMC6704323/ /pubmed/31452819 http://dx.doi.org/10.3892/ol.2019.10648 Text en Copyright: © Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Kim, Dong-Seok Kwon, Nyoun Soo Yun, Hye-Young Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non-small cell lung cancer |
title | Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non-small cell lung cancer |
title_full | Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non-small cell lung cancer |
title_fullStr | Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non-small cell lung cancer |
title_full_unstemmed | Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non-small cell lung cancer |
title_short | Leucine rich repeat LGI family member 3: Integrative analyses reveal its prognostic association with non-small cell lung cancer |
title_sort | leucine rich repeat lgi family member 3: integrative analyses reveal its prognostic association with non-small cell lung cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704323/ https://www.ncbi.nlm.nih.gov/pubmed/31452819 http://dx.doi.org/10.3892/ol.2019.10648 |
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